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Preparation for inhibiting coronavirus infections

A coronavirus and preparation technology, applied in the direction of antiviral agents, medical preparations containing active ingredients, organic active ingredients, etc. The effect of suppressing infection

Inactive Publication Date: 2016-06-22
INST OF PLA FOR DISEASE CONTROL & PREVENTION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The reality and potential threats of viral infectious diseases are becoming more and more serious for human beings, and the pressure on the prevention and control of sudden infectious diseases is increasing. When the epidemic broke out, the original specific prevention and treatment drugs were difficult to exert their due prevention and control effects, or even had no curative effect, and the limitations of human cognition and scientific research cycle made it difficult for a new generation of specific prevention and treatment drugs with significant curative effects to be introduced Launched in a short period of time, it will easily lead to the rapid spread of the epidemic and catastrophic outbreaks in some areas, which will not only cause great damage to local public health services, but also have a huge impact on society's politics and economy

Method used

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  • Preparation for inhibiting coronavirus infections
  • Preparation for inhibiting coronavirus infections
  • Preparation for inhibiting coronavirus infections

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1, the preparation of plant extract compound formula virus infection inhibitor

[0039] Epigallocatechin gallate (EGCG), tannic acid and astragalus polysaccharide are uniformly mixed according to a mass ratio of 1:1:1.5 to obtain a plant extract compound formulation.

[0040] Before the experiment, it was dissolved in PBS buffer, made into 2560 μg / ml (the total concentration of EGCG, tannic acid and astragalus polysaccharide in the solution), filtered, and after sterilization, it was stored at -20°C for later use.

Embodiment 2

[0041] Embodiment 2, cytotoxicity test

[0042] In this example, the neutral red phagocytosis method was used to measure the cytotoxicity of the compound formulation prepared in Example 1 to mammalian cells. The specific operation is as follows:

[0043] The compound formulation preparation solution (2560 μg / ml) prepared in Example 1 was diluted step by step to obtain a total of 6 concentration. Then the dilutions of different dilutions were added to the 96-well cell culture plate in which 17Cl-1 cells were cultured and the cell density was about 80%, 100 μl in each well, and 4 duplicate holes were made for each dilution, and normal cells ( That is, no compound formula preparation) was used as a control. After 2 hours of action, the test solution was discarded, and the cell maintenance culture medium was added, and 200 μl was added to each well, and placed in a cell incubator for cultivation. After 48 hours, 0.1% (0.1g / 100 mL) 25 μl of neutral red, after reacting at 37° C....

Embodiment 3

[0048] Embodiment 3, coronavirus infection inhibition test

[0049] In this embodiment, the CPE method is used to measure the inhibitory effect of the compound formulation prepared in Example 1 on coronavirus infection. The tested coronavirus is murine hepatitis coronavirus A59 strain.

[0050] Take a 96-well plate cultured with 17Cl-1 cells that have grown into a monolayer with a growth density of about 80%, pour off the culture medium, rinse the cells with PBS for 3 times, and use the three conditions of A, B, and C respectively. Add the compound formula preparation that embodiment 1 prepares:

[0051] A. Simultaneously with virus adsorption: put an equal volume of 2×100TCID 50 After mixing the virus liquid of the coronavirus with 2 times the concentration of the test substance solution, add 100 μl / well of the mixture to the cell culture plate, place it in the cell culture incubator, and discard it after the virus is adsorbed for 1 hour. After washing the cell surface 3 t...

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Abstract

The invention discloses a preparation for inhibiting coronavirus infections. The preparation provided by the invention is applied to (1) or (2) as follows: (1) preparing of products for inhibiting the coronavirus infections; (2) inhibiting of coronavirus infections. The preparation is prepared mainly through mixing epigallocatechin gallate, tannin and astragalus polysaccharides, wherein the mass proportioning ratio of the epigallocatechin gallate to the tannin to the astragalus polysaccharides is (0.5 to 1.0): (0.5 to 1.0): (0.5 to 1.5). The cytotoxicity of the compounded preparation provided by the invention is not higher than that of a control sample, i.e., ribavirin which has already obtained security permission, so that the preparation is safer. The coronavirus infections can be effectively inhibited through using the preparation provided by the invention in a preventive manner in case of safe working concentration, so that the preparation has a commercial value in being further researched and developed into a coronavirus infection inhibitor.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to a preparation for inhibiting coronavirus infection. Background technique [0002] Coronaviruses can not only cause outbreaks of many animal epidemics, but also one of the main pathogens of common colds in humans. Children have a high infection rate, mainly upper respiratory tract infections, and generally rarely spread to the lower respiratory tract. The incubation period is 2 to 5 days. All groups have the disease, more common in children, characterized by upper respiratory tract infection, a small number can cause diarrhea, bronchitis, pneumonia and pleural effusion. In recent years, it is particularly worth noting that coronaviruses often cause severe respiratory infections in humans, leading to outbreaks of severe infectious diseases such as severe acute respiratory syndrome (SARS) and Middle East respiratory syndrome (MERS), which have caused great harm to the global public health ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715A61K31/7024A61K31/353A61P31/12
CPCA61K31/353A61K31/7024A61K31/715A61K2300/00
Inventor 常国辉刘京梅杨益黄留玉罗丽晓李瑾惠罗彦军孙走南唐玥苏文莉张洁刘璇
Owner INST OF PLA FOR DISEASE CONTROL & PREVENTION
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