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Method for increasing yield of aureobasidium pullulans synthesized polymalic acid

A technology of Aureobasidium pullulans and polymalic acid is applied in the field of microbial fermentation to achieve the effects of increasing yield, improving permeability and increasing yield

Inactive Publication Date: 2016-06-01
天津北洋百川生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention patent with the application number 201310746941.5 uses the method of adding vitamins to increase the synthesis of polymalic acid, so that the output reaches 45g / L. In the prior art, there are relatively few studies on adding surfactants to improve the synthesis of products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] (1) Seed cultivation

[0046] Activate the Aureobasidium pullulans strain for 2 hours, wash the spores of the slant strain with sterile physiological saline to prepare a spore suspension, transfer the spore suspension to a 500ml baffle bottle containing 100ml seed medium according to the inoculation amount of 10% Cultivate in a medium temperature of 25°C, the rotation speed of the shaker is 200r / min, and the incubation time is 40h;

[0047] (2) Fermentation culture

[0048] The inoculation port was under flame protection, and the resulting seed solution was placed in a 5L fermenter with 3L fermentation broth in an amount of 10% (v / v) for fermentation. The initial speed is 300r / min, the ventilation ratio is 1:2, the tank pressure is 0.1Mpa, the culture temperature is set at 25°C for 0-40h, and the temperature is adjusted to 20°C after 40h, and 0.1% of the fermentation volume is added when the fermentation progresses to 48h For CTTE, 0.06% DMSO of the fermentation volum...

Embodiment 2

[0052] (1) Seed cultivation

[0053] Activate the Aureobasidium pullulans strain for 3 hours, wash the spores of the slant strain with sterile physiological saline to prepare a spore suspension, transfer the spore suspension to a 500ml baffle bottle containing 100ml seed medium according to the inoculum size of 10% Cultivate in a medium temperature of 25°C, the rotation speed of the shaker is 200r / min, and the incubation time is 40h;

[0054] (2) Fermentation culture

[0055] The inoculation port was under flame protection, and the resulting seed solution was placed in a 5L fermenter with 3L fermentation broth in an amount of 10% (v / v) for fermentation. The initial rotation speed is 300r / min, the ventilation ratio is 1:3, the tank pressure is 0.1Mpa, the culture temperature is set at 30°C for 0-40h, and the temperature is adjusted to 25°C after 40h, and 0.12% of the fermentation volume is added when the fermentation progresses to 48h For CTTE, 0.08% DMSO of the fermentation ...

Embodiment 3

[0059] (1) Seed cultivation

[0060] Activate the Aureobasidium pullulans strain for 2.5 hours, wash the spores of the slant strain with sterile physiological saline to prepare a spore suspension, transfer the spore suspension to a 500ml baffle containing 100ml seed medium according to the inoculum size of 10% Cultivate in a bottle, the culture temperature is 25°C, the shaker speed is 200r / min, and the culture time is 40h;

[0061] (2) Fermentation culture

[0062] The inoculation port was under flame protection, and the resulting seed solution was placed in a 5L fermenter with 3L fermentation broth in an amount of 10% (v / v) for fermentation. The initial rotation speed is 300r / min, the ventilation ratio is 1:1, the tank pressure is 0.1Mpa, the culture temperature is set at 35°C for 0-40h, and the temperature is adjusted to 30°C after 40h, and 0.14% of the fermentation volume is added when the fermentation progresses to 48h For CTTE, 0.1% DMSO of the fermentation volume was a...

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PUM

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Abstract

The invention discloses a method for increasing the yield of aureobasidium pullulans synthesized polymalic acid. The method comprises the following steps: culturing seeds, and performing fermental cultivation, namely, adding an active agent consisting of CTTE and DMSO in the fermentation process in different stages, controlling the temperature of the fermentation process by using a stage temperature control method, setting the cultivation temperature to 25-35 DEG C for 0-40 hours, adjusting the temperature to 20-30 DEG C after 40 hours, flowing 0.1-0.14% of CTTE at a logarithmic growth stage in the fermentation process, and flowing 0.06-0.1% of DMSO at the middle fermentation stage. By the adoption of the method, the yield of polymalic acid is increased by improving permeability of cell membranes, the fermentation period is also shortened, the yield of polymalic acid is generally increased by 40%, the production cost is reduced, and the production efficiency is improved.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a method for increasing the yield of polymalic acid synthesized by Aureobasidium pullulans. Background technique [0002] Aureobasidium pullulans is a half-known fungus belonging to the genus Aureobasidium, also known as "Puldella", "Puculus lateralis", "Aureobasidium pullulans" and "Aureobasidium", which is a species closely related to yeast. , Polymorphic fungi with yeast-type and hyphae-type morphology. Polymalic acid, antibiotics, enzymes, extracellular polysaccharides and single-cell proteins can be obtained by fermentation with Aureobasidium pullulans. [0003] Polymalic acid is a homopolymer polymer synthesized from malic acid as the only monomer. Its special three-dimensional structure makes it have excellent properties such as biocompatibility, biodegradability and bioabsorbability. Second, as a water-soluble aliphatic polyester, it is highly water-solub...

Claims

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Application Information

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IPC IPC(8): C12P7/46C12R1/645
Inventor 乔长晟边艳慧李雪范栩嘉
Owner 天津北洋百川生物技术有限公司
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