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Method for producing low-polarity ginsenoside

A ginsenoside, low-polarity technology is applied in the field of producing low-polarity ginsenosides, which can solve the problems of large batch differences, inability to guarantee product stability, and inability to maintain constant ginseng pyrolysis products and quantities.

Inactive Publication Date: 2016-06-01
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The patent only controls the temperature, the quality and quantity of ginseng pyrolysis products cannot be kept constant, the product stability cannot be guaranteed, the content of rare ginsenosides in the product is low, and the batch difference is large

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] 4-year-old cultivated ginseng roots (dried ginseng with purple stem and red fruit, raw sun-dried ginseng 5000g, water content 5-12%) were added to 50L of probiotics (Lactobacillus bulgaricus, Streptococcus thermophilus, Paecilomyces spp. , and the number of yeasts are 10 2 cfu, 10 2 cfu, 10 2 cfu, 10 2 cfu) in the catalytic decomposition liquid, fermented at 37°C for 24 hours, and heated at 100°C for 30 minutes. HPLC analysis of the converted product showed that the conversion rate was 90.3%. The main products are Rg3, Rh2, protopanaxadiol, protopanaxatriol, panaxadiol, panaxatriol, 25-OH-dammarane, 25-OH-dammarene and other rare ginsenosides and aglycones and natural ginseng saponins. Transformed ginseng was crushed, refluxed and extracted with methanol three times, methanol was removed under reduced pressure, and evaporated to dryness. Add 30% ethanol to the evaporated matter to wash repeatedly, remove ginseng polysaccharide, and dry the precipitate. Dissolved ...

Embodiment 2

[0087] Diol-type ginsenoside (50g) (commercially purchased from Jilin Zixin Chuyuan Pharmaceutical Co., Ltd.) was added to 50L of 4 strains of lactic acid bacteria (Lactobacillus bulgaricus, Lactobacillus acidophilus, Streptococcus thermophilus, Bifidobacterium according to the quality of 1g than mix 10 2 cfu, 10 2 cfu, 10 2 cfu, 10 2 cfu) were mixed, catalytically decomposed for 24 hours, and heated at 100°C for 30 minutes. HPLC analysis of the conversion product showed that the conversion rate was 94%. The main products are Rg3, Rh2, protopanaxadiol, panaxadiol, 25-OH-dammarane, 25-OH-dammarene rare ginsenosides and aglycones. The catalytic decomposition liquid was collected by centrifugation, dissolved in methanol, centrifuged, and the supernatant was evaporated to dryness, and 30% ethanol was added to the evaporated matter for repeated washing to remove ginseng polysaccharides, and the precipitate was dried. Dissolved with methanol, reversed-phase preparation column t...

Embodiment 3

[0105] Diol type ginsenoside (commercially purchased from Jilin Zixin Chuyuan Pharmaceutical Co., Ltd.) (50g) and glucosidase (Shanghai

[0106] Jiahe Biotechnology Co., Ltd., the enzyme activity is 300U / g) 5g was added to 500ml water, and reacted at 40°C for 16 hours

[0107] , heat at 100°C for 30 minutes to inactivate. The HPLC analysis of conversion product shows, transformation efficiency 72%, product mainly

[0108] The main products are Rg3, Rh2, protopanaxadiol, 25-OH-dammarane and 25-OH-dammarene rare ginseng

[0109] Saponins and aglycones. Add 5000mL of water to form a suspension, centrifuge, dissolve the precipitate with methanol, and evaporate the supernatant to dryness.

[0110] The evaporated matter was rinsed with 30% ethanol to remove impurities, and then evaporated to dryness. dissolved in methanol and separated by a reversed-phase preparative column (flow

[0111] Phase is 65% ethanol) to obtain Rg3, Rh2, protopanaxadiol, 25-OH-dammarane and 25-OH-damma...

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Abstract

The invention discloses a method for producing low-polarity ginsenoside. The method comprises the following steps: performing catalytic decomposition of the raw materials: a ginsenoside-containing plant, a tissue culture of the plant, an extract of the plant and / or natural ginsenoside; and heating and separating to obtain the low-polarity ginsenoside. By adopting the ginseng processing method disclosed by the invention, 25-hydroxyl dammarane and 25-dammarene can be produced; and by carefully controlling the catalytic decomposition process, the output and composition of the extracted ginsenoside are controllable, and the yield of the target product extracted from the raw materials such as treated ginseng is high.

Description

technical field [0001] The invention relates to a method for producing low-polarity ginsenosides, belonging to the field of natural product processing. Background technique [0002] Ginseng is the dry root of the Araliaceae plant ginseng, which mostly grows in mixed coniferous and broadleaf forests or miscellaneous forests on gentle slopes or slopes of mountains with a small temperature difference between day and night at an altitude of 500-1100 meters. Because the root is hypertrophy, shaped like a spindle, often has bifurcations, and its overall appearance resembles a human head, hands, feet and limbs, it is called ginseng. Ginseng is known as the "King of Herbs". For a long time, due to over-excavation and resource depletion, the forest ecological environment on which ginseng depends has been severely damaged. At present, Northeast ginseng is also on the verge of extinction. The wild ginseng in the Northeast is also extremely rare, therefore, the protection of the natur...

Claims

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Application Information

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IPC IPC(8): C07J17/00
CPCC07J17/005
Inventor 孙妙囡孙德军
Owner JILIN UNIV
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