Method for measuring millet seed vigor based on ultraviolet spectrophotometer technology

Abstract

The invention discloses a method for measuring millet seed vigor based on an ultraviolet spectrophotometer technology. The light absorption value of a seed lixivium is determined by adopting an ultraviolet spectrophotometer detection technology according to the principle of amino acid exosmose because the integrity of a cell membrane is damaged during a storage process of seeds; the storage years are more, and the exosmosis organic electrolytes are more, so that the light absorption value is higher. According to the method, the millet seed vigor can be detected quickly; the sample soaking time are found, and the sample determination wavelengths are screened to determine a seed vigor standard; the detection accuracy is high; the time consumption is short; the millet seeds with strong vigor have a low light absorption value; the millet seeds with weak vigor have a strong amino acid outflow effect and have a high light absorption value; the evaluation method is simple and reliable.

Description

technical field [0001] The invention relates to the field of methods for measuring seed vigor, in particular to a method for measuring the vigor of millet seeds based on ultraviolet spectrophotometer technology. Background technique [0002] Peeled millet is called millet. Its grain is rich in vitamins, minerals, fat and dietary fiber, which can prevent and treat hyperlipidemia, hyperglycemia, soften blood vessels and other diseases. It is a healthy food with the same source of medicine and food. The carbohydrate content of millet is far lower than that of bulk crops, making it the first choice for diabetic patients. Millet is rich in mineral elements, among which calcium and iron are high in content, making it an ideal food for women's postpartum recovery, as well as a nutritious food for children and patients . [0003] Seed vigor is the sum of seed germination rate, seedling growth potential, plant stress resistance and production potential. It can directly reflect the ...

AI Technical Summary

Problems solved by technology

The above method of measuring seed vigor mainly relies on manual operation, the steps are complicated, the workload is heavy, the cycle is long, and the seeds are damaged, so that the tested seeds can no longer be used for production.

New methods such as near-infrared spectroscopy, laser speckle technology, infrared thermal imaging technology, and PCR detection technology developed in recent years have the advantages of simplicity, no pretreatment, no pollution, no damage, and low cost. However, due to the single detection index , can not reflect the seed vigor well

[0005] Millet seeds belong to the category of small seeds, with an average grain size of 0.3cm in length, 0.2cm in width, and 0.1cm in height. It is difficult to cut the embryo completely, and the TTC quantitative method is limited. It usually takes 5-7 days to get the results, which takes a long time; the conductivity method is greatly affected by factors such as room temperature, water temperature, seed quality, and water quality, and the error is large

At present, there are few reports on identification methods for the vigor of millet seeds

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