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Lactobacillus plantarum having anti-diabetic function and application thereof

A technology of Lactobacillus plantarum and anti-diabetes, which is applied in the biological field and can solve problems such as large differences in probiotic activity

Inactive Publication Date: 2016-05-11
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the strain-specific probiotic activity of lactic acid bacteria, the probiotic activity of different strains varies greatly

Method used

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  • Lactobacillus plantarum having anti-diabetic function and application thereof
  • Lactobacillus plantarum having anti-diabetic function and application thereof
  • Lactobacillus plantarum having anti-diabetic function and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Screening and preservation of bacterial strains.

[0029] The Lactobacillus plantarum NCU116 of the present invention is obtained by separation and screening from traditional Chinese kimchi old liquid, and the specific separation and identification steps are as follows:

[0030] (1) Isolation and cultivation of strains.

[0031] The traditional Chinese kimchi old solution from different sources was inoculated into the vegetable juice enrichment medium according to the inoculum amount of 3%, and anaerobically cultured at 37°C for 24h. Pipette 1mL of the vegetable juice enrichment culture solution, adopt the 10-fold gradient dilution method, select an appropriate dilution, spread it on the primary screening plate medium, and culture it anaerobically at 37°C for 48 hours. , large colonies, and large yellow bacterial circles. The strains selected by the primary screening were transferred to the secondary screening plate medium and cultured anaerobically at 37°C...

Embodiment 2

[0050] Example 2: Gastrointestinal tolerance test of Lactobacillus plantarum NCU116.

[0051] By simulating the human digestive tract environment, the survival ability of Lactobacillus plantarum NCU116 in the gastrointestinal tract was explored, so as to provide a reference for whether this strain of lactic acid bacteria can play a health-promoting role in the human digestive tract environment.

[0052] (1) Artificial gastric juice tolerance.

[0053] Take the freeze-dried bacterial powder of Lactobacillus plantarum NCU116, inoculate 1% of the inoculum into artificial gastric juice with pH values ​​of 1.5, 2.5, 3.5 and 4.5, mix well, and shake at 100r / min at 37°C Cultivate, take samples after 0, 1, 2 and 3 hours respectively, count live lactic acid bacteria, and measure the light transmittance at a wavelength of 600nm.

[0054]

[0055] (2) Tolerance of artificial intestinal juice.

[0056] Take the freeze-dried bacterial powder of Lactobacillus plantarum NCU116, inoculat...

Embodiment 3

[0063] Example 3: Adhesion performance test of Lactobacillus plantarum NCU116.

[0064] Simulating the human intestinal environment, the in vitro cell model human colon cancer cell line HT-29 was used to investigate the adhesion of Lactobacillus plantarum NCU116, and the effects of different factors on the adhesion of NCU116 to HT-29 were studied. The human intestinal environment is neutral, so the sterile PBS buffer solution with pH 7.30 is used to simulate the intestinal environment, and the bacterial concentration is adjusted to 1.0×10 8 CFU / mL, co-incubated with HT-29 cells for 2.0 hours in an anaerobic incubator at 37°C, the number of adhered bacteria was 4.78±0.21CFU / cell (number of adherent bacteria / HT-29), the adhesion ability of Lactobacillus plantarum NCU116 strong.

[0065] (1) Use sterile PBS buffer (pH7.30) to adjust the concentration of bacteria to 1×10 5 , 1×10 6 , 1×10 7 , 1×10 8 , 1×10 9 CFU / mL, co-incubated with the cells for 2.0h, calculated the number...

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Abstract

Lactobacillus plantarum having an anti-diabetic function and application thereof are disclosed, the strain is Lactobacillus plantarum NCU116, and the accession number is CGMCC 11885. The strain has a function to alleviate symptoms of diabetes, and the composition is in a form of food or a pharmaceutical composition. The present invention also discloses a method for screening and identification of the lactic acid bacteria strain as well as a method to achieve the anti-diabetic effect by regulating blood sugar, blood lipids and hormone levels and regulating metabolic pathways in the body. The daily-intake lactic acid bacteria or a composition thereof is used to replace a medicine to achieve the anti-diabetic effect; and the healthy daily-intake lactic acid bacteria or the composition thereof is free of toxic and side effects to human body is used as a new choice to alleviate the symptoms of diabetes.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to plant lactobacillus and application thereof. Background technique [0002] Probiotics refer to a class of active microorganisms that have beneficial effects on the host. By colonizing the host's intestinal tract and reproductive system, they can produce definite health effects on the host, thereby improving the micro-ecological balance in the host and exerting beneficial effects. general term. As the most representative genus of probiotics, lactic acid bacteria can help digestion, promote the absorption of nutrients, relieve lactose intolerance, prevent and treat diarrhea, regulate intestinal flora and relieve constipation, regulate the body's immune function, reduce blood Total cholesterol, improvement of allergic diseases caused by food, anti-aging and anti-tumor and other physiological activities. [0003] Type II diabetes is a kind of metabolic disorder disease caused by insulin ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L33/135A61K35/747A61P3/10C12R1/25
CPCC12N1/20A23V2002/00A61K2035/11C12R2001/25C12N1/205A23V2400/169A23V2200/328
Inventor 谢明勇熊涛宋苏华帅高平关倩倩
Owner NANCHANG UNIV
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