Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Glucooligosaccharide and preparation method and application thereof

A technology of oligoglucosaccharide and glucose, which is applied in the field of agricultural application, can solve the problems of long enzymatic hydrolysis cycle, low efficiency and high production cost, and achieves the effects of low cost, simple preparation process, and easy separation and purification

Active Publication Date: 2016-05-11
NANJING SOUTHERN ELEMENT BIOTECHNOLOGY CO LTD
View PDF7 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing enzymatic hydrolysis method is limited by the source and type of enzyme, and generally has the disadvantages of long enzymatic hydrolysis cycle, low efficiency and high production cost.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Glucooligosaccharide and preparation method and application thereof
  • Glucooligosaccharide and preparation method and application thereof
  • Glucooligosaccharide and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] This example illustrates the method of obtaining glucooligosaccharides through mixed culture and fermentation of Agrobacterium ZX09 and Paenibacillus S09.

[0033] a. Preparation of seed liquid: Inoculate a single colony of ZX09 bacteria into an inorganic salt-sucrose liquid medium, and culture it with shaking at 30°C for 24 hours. The medium composition is: 20g sucrose, 0.5g sodium nitrate, 0.5g potassium dihydrogen phosphate, Anhydrous calcium chloride 0.1g, magnesium sulfate heptahydrate 0.3g, ferrous sulfate heptahydrate 0.02g, zinc chloride 0.003g, manganese sulfate 0.001g, water 1000mL, pH7.0. Autoclave at 121℃ for 20min. Inoculate a single colony of S09 bacteria into LBO liquid medium, shake and culture at 35°C for 24h, the medium composition is 0.5g of sodium chloride, 0.5g of yeast extract, 1.0g of peptone, 0.5g of oat flour, and sterilize by high pressure steam at 121°C 20min.

[0034] b. Mixed bacteria culture: Inoculate the seed liquid of the cultivated ZX0...

Embodiment 2

[0038] Preparation of oligoglucose derivatives.

[0039] Prepare the purified oligosaccharides into 10mg / mL aqueous solution, add NaOH with a final concentration of 0.2M to react for 2-5h, neutralize with HCl, desalt through SephadexG25, collect the eluted peak samples of oligosaccharides, and obtain the removal Oligodextrose derivative II with succinyl modification group. The purified oligoglucose was prepared into an aqueous solution of 10 mg / mL, added HCl with a final concentration of 0.5M to react for 5 hours, neutralized with NaOH, desalted through SephadexG25, collected eluted peak samples of oligoglucose, and obtained amber removal at the same time Acyl- and pyruvate-modified oligodextrose derivatives III.

Embodiment 3

[0041] Control of Botrytis cinerea infection in Arabidopsis by gluco-oligosaccharides.

[0042] Prepare a 0.2-1.0 g / L gluco-oligosaccharide aqueous solution, and spray evenly on the leaves of Arabidopsis plants that have grown to a suitable size, spray once a day, and spray with clear water as a control. Two days after the pretreatment of the gluco-oligosaccharide, the cultured Botrytis cinerea was punched on the solid medium, and the mold agar block was pasted on the leaves of Arabidopsis thaliana for infection culture. After 1-3 days, observe the degree of infection of the leaves of the plants in the control group and the gluco-oligosaccharide group.

[0043] The results showed that spraying the gluco-oligosaccharide solution in advance could reduce the infection rate of Arabidopsis plant leaves, and the plaques of the bacteria were significantly smaller. image 3 Shown are the leaves of Arabidopsis plants after spraying clear water and spraying 0.5g / L gluco-oligosaccharide...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
Login to View More

Abstract

The invention discloses glucooligosaccharide with succinic acid and pyruvoyl modified groups and a de-modified derivative of the glucooligosaccharide. A main chain of the glucooligosaccharide is composed of eight glycosyls and is free of branched chain connection, and the glycosyls of the main chain are connected by one alpha-1, 3-glycosidic bond and six beta-1, 3 glycosidic bonds. The glucooligosaccharide is obtained by directly separating from fermentation supernate after passing liquid mixed fermentation Agrobacterium ZX09 and Paenibacillus S09, and a glucooligosaccharide dry powder product is obtained after subjecting the fermentation supernate to alcohol precipitation, centrifuging and drying. The preparation method is quick, effective, simple in process, high in yield, low in cost, free of pollution and suitable for industrialized production. The glucooligosaccharide prepared by the method is high in water solubility, uniform in molecular weight and structure, easy to prepare and purify, high in bioactivity and capable of effectively activating resistant, to pathogenic fungi, of plants, can serve as a plant induced resistance agent and a plant protective agent, has resistance inducing effect on crops remarkably better than that of kelp oligosaccharide and has wide application value in the aspects of prevention and control of plant diseases and pests.

Description

technical field [0001] The invention belongs to the technical field of agricultural application, and in particular relates to a glucooligosaccharide and its derivatives prepared by microbial mixed fermentation, a preparation method of the glucooligosaccharide and its application in plant induction and plant protection. Background technique [0002] Oligosaccharides, also known as oligosaccharides or oligosaccharides, are generally 3 to 10 monosaccharides connected by glycosidic bonds with straight or branched sugars. According to whether it can be metabolized and absorbed by the human body and whether it has biological functions, oligosaccharides can be divided into ordinary oligosaccharides and functional oligosaccharides. Ordinary oligosaccharides such as sucrose, maltose, lactose and trehalose can be digested and absorbed by the human body. They are energy substances and have no special physiological functions. Functional oligosaccharides generally have physical and chem...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/02C12P39/00C12P19/08A01N43/90A01N43/16A01P21/00
CPCA01N43/16A01N43/90C08B37/0024C12P19/08C12P39/00
Inventor 张建法程瑞李晶
Owner NANJING SOUTHERN ELEMENT BIOTECHNOLOGY CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com