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Food material-based cordyceps militaris mycelium solid culture method and solid culture medium utilized in solid culture method

A solid medium, Cordyceps sinensis technology, applied in the directions of microorganism-based methods, fungi, biochemical equipment and methods, etc., can solve the problems of long solid culture time, affecting product palatability, chemical residues, etc. It takes time, good loose air permeability, good absorption effect

Active Publication Date: 2016-05-04
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these auxiliary materials are not normal ingredients. The silkworm chrysalis has a special smell that will affect the palatability of the product, and some will cause chemical residues
In addition, because Cordyceps sinensis is an aerobic bacterium, its mycelium grows on the surface of grain particles in the gaps of the solid medium, and the general solid culture time is relatively long, such as the solid culture time disclosed in the invention patent with application number 200910197957.9. up to 8 to 12 weeks

Method used

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  • Food material-based cordyceps militaris mycelium solid culture method and solid culture medium utilized in solid culture method
  • Food material-based cordyceps militaris mycelium solid culture method and solid culture medium utilized in solid culture method
  • Food material-based cordyceps militaris mycelium solid culture method and solid culture medium utilized in solid culture method

Examples

Experimental program
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preparation example Construction

[0064] The preparation method of Cordyceps sinensis secondary seed liquid (secondary strain) carries out the following steps successively:

[0065] Under aseptic operation conditions, pick the slant lawn of Cordyceps sinensis——Cordyceps sinensis slant bacteria species and inoculate it on a PDA plate, and cultivate it at 15-25°C for 5 days to obtain an activated lawn of Cordyceps sinensis; pick Cordyceps sinensis The activated bacterial lawn was inoculated in PDA liquid medium, and cultured with shaking (100-200r / min) at 18-22°C for 5 days to obtain the first-class seed liquid of Cordyceps sinensis (first-class seed bacterial liquid); press 10% (v / v ) Inoculation amount Inoculate the first-level seed bacterial liquid into the PDA liquid culture liquid, and cultivate it for 5 days with shaking (100-200r / min) at 18-22°C to obtain the second-level strain (the second-level seed liquid of Cordyceps sinensis) for use ( placed at 4°C for later use).

[0066] Among the secondary strai...

Embodiment 1

[0068] 1. Cordyceps sinensis mycelium solid medium and preparation method, the formula of the medium is composed of the following ingredients by weight percentage:

[0069]

[0070] The preparation method of this culture medium, implement as follows:

[0071] ①. The non-glutinous yellow millet is washed with tap water and used as the main ingredient of the medium;

[0072] ②, the washed peeled potatoes and rabbit meat are crushed and homogenized to obtain a slurry;

[0073] ③. Mix the slurry prepared from main and auxiliary materials after washing with sucrose and water, and cook mature rice with an automatic electric cooker;

[0074] ④. Divide the cooked rice into 15cm Petri dishes, sterilize at 121°C for 20 minutes, and cool to obtain a solid medium for use.

[0075] Two, Cordyceps sinensis mycelium solid culture method, carry out following steps successively:

[0076] ①. First spray 75% (volume %) alcohol on the inner wall of the incubator, and then start the ozone ge...

Embodiment 2

[0084] The formula of the Cordyceps sinensis mycelium solid medium consists of the following ingredients by weight:

[0085]

[0086] All the other contents are equal to embodiment 1 (that is, the preparation method of solid medium, the solid culture method of Cordyceps sinensis mycelia are equal to embodiment 1.)

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Abstract

The invention discloses a cordyceps militaris mycelium solid culture medium. The solid culture medium comprises the following components in percentage by weight: 30%-50% of main materials with the particle sizes less than or equal to 2mm, 0.1%-15% of livestock, 0.1%-15% of vegetables, 0.1%-15% of saccharides and the balance of water, wherein the main materials are non-glutinous cereals or beans, the livestock and the vegetables are auxiliary materials, and the saccharides are monosaccharides or disaccharides. The invention further simultaneously discloses a preparation method of the cordyceps militaris mycelium solid culture medium and a food material-based cordyceps militaris mycelium solid culture method utilizing the solid culture medium. By taking the food materials as the main materials and the auxiliary materials, the solid culture medium has relatively large specific surface area and good loosening breathability, and good nutritional conditions and physical space can be provided for the growth of cordyceps militaris mycelia.

Description

technical field [0001] The invention belongs to the field of biological material cultivation methods, and in particular relates to a solid culture medium and a cultivation method for Cordyceps sinensis mycelia. Background technique [0002] Cordyceps sinensis (Ophiocordyceps sinensis) is a complex of insects and fungi formed by the larvae of the Lepidoptera bat moth family Batmoth genus parasitized by the Chinese spore (Hirsutellasinensis). Dating back thousands of years. According to previous editions of the "Pharmacopoeia of the People's Republic of China", its main effects are to nourish the kidney and lung, stop bleeding and resolve phlegm; it is used to treat kidney deficiency and essence deficiency, impotence and nocturnal emission, soreness of the waist and knees, chronic cough and dyspnea due to deficiency, and hemoptysis due to fatigue. Modern medical and pharmacological studies have proved that Cordyceps sinensis has a variety of therapeutic and nourishing health ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 方萍谢媛
Owner ZHEJIANG UNIV
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