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Food-based solid culture method of Cordyceps sinensis mycelium and solid medium used

A technology of solid medium and Cordyceps sinensis, applied in the direction of microorganism-based methods, fungi, biochemical equipment and methods, etc., can solve the problems of long solid culture time, affecting product palatability, chemical residues, etc., and reduce the cost of culture Time-consuming, good loose air permeability, good for absorption effect

Active Publication Date: 2018-12-14
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these auxiliary materials are not normal ingredients. The silkworm chrysalis has a special smell that will affect the palatability of the product, and some will cause chemical residues
In addition, because Cordyceps sinensis is an aerobic bacterium, its mycelium grows on the surface of grain particles in the gaps of the solid medium, and the general solid culture time is relatively long, such as the solid culture time disclosed in the invention patent with application number 200910197957.9. up to 8 to 12 weeks

Method used

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  • Food-based solid culture method of Cordyceps sinensis mycelium and solid medium used
  • Food-based solid culture method of Cordyceps sinensis mycelium and solid medium used
  • Food-based solid culture method of Cordyceps sinensis mycelium and solid medium used

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0064] The preparation method of Cordyceps sinensis secondary seed liquid (secondary bacterial species) is as follows:

[0065] Under aseptic conditions, pick the Cordyceps sinensis slant moss-----Chinese tortiella (Cordycepssinensis) slant strains and inoculate it on a PDA plate, culture at 15~25℃ for 5 days to get the Cordyceps sinensis activated moss; pick Cordyceps sinensis The activated bacteria lawn was inoculated into PDA liquid medium, and cultured at 18-22°C with shaking (100-200r / min) for 5 days to obtain the first-level seed liquid of Cordyceps sinensis (first-level seed bacteria liquid); press 10% (v / v) ) Inoculum amount: Inoculate the first-level seed bacterial solution in the PDA liquid culture solution, and incubate at 18-22°C with shaking (100-200r / min) for 5 days to obtain the second-level bacterial species (secondary seed solution of Cordyceps sinensis), and set aside ( Put it at 4°C for later use).

[0066] In this secondary species, the content of Cordyceps sin...

Embodiment 1

[0068] 1. Cordyceps mycelium solid culture medium and preparation method, the formula of the culture medium is composed of the following ingredients by weight percentage:

[0069]

[0070] The preparation method of the medium is implemented as follows:

[0071] ① Rinse non-glutinous yellow millet with tap water as the main ingredient of the culture medium;

[0072] ②. The washed peeled potatoes and rabbit meat are crushed and homogenized to obtain slurry;

[0073] ③. Mix the slurry prepared from the main ingredients and auxiliary materials after washing with sucrose and water, and cook the mature rice in an automatic rice cooker;

[0074] ④. Dispense the cooked rice into 15 cm petri dishes, sterilize at 121°C for 20 minutes, and cool down to obtain a solid medium for use.

[0075] 2. Cordyceps sinensis mycelium solid culture method, followed by the following steps:

[0076] ① Spray 75% (vol%) alcohol on the inner wall of the incubator first, and then start the ozone generator for 30 minut...

Embodiment 2

[0084] The formula of the cordyceps mycelium solid medium is composed of the following ingredients by weight:

[0085]

[0086] The rest of the content is the same as in Example 1 (that is, the preparation method of solid medium and the solid culture method of cordyceps mycelium are equivalent to Example 1.)

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Abstract

The invention discloses a cordyceps militaris mycelium solid culture medium. The solid culture medium comprises the following components in percentage by weight: 30%-50% of main materials with the particle sizes less than or equal to 2mm, 0.1%-15% of livestock, 0.1%-15% of vegetables, 0.1%-15% of saccharides and the balance of water, wherein the main materials are non-glutinous cereals or beans, the livestock and the vegetables are auxiliary materials, and the saccharides are monosaccharides or disaccharides. The invention further simultaneously discloses a preparation method of the cordyceps militaris mycelium solid culture medium and a food material-based cordyceps militaris mycelium solid culture method utilizing the solid culture medium. By taking the food materials as the main materials and the auxiliary materials, the solid culture medium has relatively large specific surface area and good loosening breathability, and good nutritional conditions and physical space can be provided for the growth of cordyceps militaris mycelia.

Description

Technical field [0001] The invention belongs to the field of biological material cultivation methods, and particularly relates to a solid culture medium and cultivation method for cordyceps mycelium. Background technique [0002] Cordyceps sinensis (Ophiocordyceps sinensis) is an insect-bacterial complex formed by the parasitism of the larvae of the Hepialidae Hepteridae of the Lepidoptera Hepidiae family by Hirsutella sinensis. It has been circulated and used in my country for more than two thousand years. Records can be traced back to thousands of years ago. According to the historical version of "The Pharmacopoeia of the People's Republic of China", its main effect is to invigorate the kidneys and lungs, stop bleeding and resolve phlegm; it is used to treat kidney deficiency, impotence and nocturnal emission, waist and knee pain, chronic cough, wheezing, and hemoptysis. Modern medicine and pharmacological studies have proved that Cordyceps has a variety of therapeutic and nour...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 方萍谢媛
Owner ZHEJIANG UNIV
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