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Industrial preparation method of quercetagetin

A technology for quercetin marigold and marigold slag, which is applied in the field of industrialized preparation of quercetin marigold, can solve the problems of difficult industrialized production, unmentioned product yield, etc. effect of continuity

Inactive Publication Date: 2016-05-04
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are few reports on quercetin at home and abroad. ZL201110075820 discloses a preparation method of high-purity quercetin. Countercurrent chromatography is used for liquid-liquid extraction to separate and purify quercetin. Although its purity reaches 95%, but did not mention the yield of the product; a large number of organic solvents (chloroform, methanol) were used in the patented process, and the standard and removal method of solvent residues in the final product were not mentioned; the patented process technology is more difficult Realize industrial production

Method used

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  • Industrial preparation method of quercetagetin
  • Industrial preparation method of quercetagetin
  • Industrial preparation method of quercetagetin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1) Extraction: Weigh 15kg of fermented marigold flower residue, add 100kg of alcohol (70%, v / v) to mix, and stir at room temperature for 30min to form an extraction system; raise the temperature of the extraction system to 60°C, and use ultrasonic waves to assist The extraction method is to extract, the ultrasonic frequency is 20kHz, the ultrasonic power is 400W, the ultrasonic emission frequency is 20s / 2s / 20s, and the extraction time is 2.5h. After the extraction is completed, the quercetin-tagerein crude extract is obtained;

[0031] 2) Centrifugal filtration: centrifuge the obtained quercetin-tagalin crude extract for 400 s in a three-legged centrifuge (300 mesh filter cloth) at a speed greater than 6200 rpm; then perform plate-and-frame filter press on the supernatant, and wash with a small amount of alcohol Plate and frame 3 times to obtain filter cake and quercetin clarified solution;

[0032] 3) Concentration: Concentrate the obtained quercetin clarified liquid a...

Embodiment 2

[0040] 1) Extraction: Weigh 15kg of marigold flower residue after extracting lutein, add 100kg of alcohol (60%, v / v) to mix, stir at room temperature for 30min and mix evenly to form an extraction system; heat the extraction system to 50°C , using ultrasonic-assisted extraction method for extraction, the ultrasonic frequency is 20kHz, the ultrasonic power is 400W, the ultrasonic emission frequency is 20s / 2s / 20s, the extraction time is 1h, after the extraction is completed, the quercetin-tagetin crude extract is obtained;

[0041] 2) Centrifugal filtration: centrifuge the obtained quercetin-tagetine crude extract for 500 s in a three-legged centrifuge (300 mesh filter cloth) at a rotation speed greater than 6200 rpm; perform plate-and-frame filter on the supernatant, and use a small amount of alcohol plate-and-frame Washing 3 times to obtain filter cake and quercetin clarified solution;

[0042] 3) Secondary extraction: Add 90kg of alcohol (60%, v / v) to the filter cake and mix ...

Embodiment 3

[0046] Example 3: Activity Analysis of Quercetin Tagetes Products

[0047] The quercetin tagetin obtained in Example 1 was subjected to in vitro scavenging of DPPH and ABTS free radicals and inhibition of α-glucosidase and α-amylase, and the results are shown in Table 2. The results showed that, compared with the reference substance, the quercetin tagetin obtained by this method had good antioxidant and inhibitory activities of glucosidase and amylase.

[0048] Table 2: IC of Quercetin Tagetes in Scavenging Free Radicals and Inhibiting Enzyme Activity 50 Value (mg / L)

[0049]

[0050] Note: ND: not determined, there are significant differences in the data with different letters superscripted in the same column (P<0.05).

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Abstract

The invention discloses an industrial preparation method of quercetagetin and belongs to the technical field of extraction and preparation of flavonoid compounds. Marigold residues are uniformly mixed with alcohol, the mixture is heated to the extraction temperature and extracted in an ultrasonic-assisted extraction manner, then, centrifugal filtration, concentration, centrifugal filtration and purification are performed, and finally, a quercetagetin product is obtained. According to the preparation method, waste marigold residues are taken as a raw material, and resource waste and environment pollution are reduced; the extraction is performed in the ultrasonic-assisted extraction manner, and the production continuity is conveniently and effectively guaranteed on the basis of high efficiency and energy conservation; the production energy consumption is reduced by means of a normal-temperature water washing and purification processing technology, and the production cost is reduced; the purity of the quercetagetin product extracted with the method is higher than 85%, and the yield is 2.5%-3.8%.

Description

technical field [0001] The invention belongs to the technical field of extraction of flavonoids, and in particular relates to an industrial preparation method of quercetin tagetes. Background technique [0002] The complete processing of agricultural products has always been the goal that food processing companies expect to achieve. At present, the plant residues produced by food additive processing enterprises to produce natural pigments and other products from plant raw materials, except a small part for feed processing, most of the rest are wasted and cause environmental pollution. The use of plant residues to produce high value-added products such as dietary fiber, flavonoids or polyphenols, and pectin polysaccharides can extend the industrial chain of agricultural product processing, increase the added value of plant deep-processing products, and realize the comprehensive utilization of resources. [0003] Flavonoids are a class of food nutrients with health functions....

Claims

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Application Information

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IPC IPC(8): C07D311/30
CPCC07D311/30
Inventor 高彦祥许洪高王维有
Owner CHINA AGRI UNIV
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