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Method for preparing biological zinc from oysters

A bio-zinc and oyster technology, applied in the direction of food science, etc., can solve the problems of low bio-absorption utilization rate, slow absorption, and different molecular weights of free bio-zinc, and achieve the effect of ensuring high bio-absorption rate

Inactive Publication Date: 2016-04-13
GUANGXI PANDA BIOTECH DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, Chinese patent CN201010234452.8 discloses a method for synchronously extracting taurine, biological zinc and polypeptide from oysters, obtaining biological zinc through ultrasonic crushing, boiling, resin purification, concentration, etc. and the academic paper "Food Development and Research" " Research on the Extraction and Preparation of Biological Zinc from Near River Oysters" (July 2015, Volume 36, No. 14) discloses the method of preparing biological zinc through ultrasonic crushing. The disadvantage of this method is that the ultrasonic crushing method uses high frequency When the mechanical wave acts on the solution, bubbles are generated, and the bubbles grow and break to form cavitation, and the shock wave and shear force caused by the cavitation cause the cells to rupture
The shock wave and shear force caused by the ultrasonic itself have no directionality and molecular cracking ability, so only free biological zinc can be released, and most of the biological zinc with zinc protein structure cannot be released, and the molecular weight of free biological zinc is different, resulting in biological Disadvantages such as low absorption utilization rate and slow absorption

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] An example of the method of utilizing oysters to prepare biological zinc according to the present invention comprises the following steps:

[0027] Wash and shell 35kg of live oysters, add 2 times of purified water at 3°C, add 0.05w / w% lysozyme with an activity of 15,000 / g from egg white, and grind it into a fine powder in a colloid mill with a rotation speed of 1500r / min. The density is 0.15mm, placed in the bioreactor, adding 0.3w / w% activity of 200,000 U / g cellulase, 1.5w / w% activity of 30,000 U / g fungal proteolytic enzyme and 0.5w / w The % activity is 80,000 U / g lipase. After reacting for 10 hours at a temperature of 35°C and a rotating speed of 150r / min, the centrifugal radius is 800mm and the rotating speed is 2500r / min for 15 minutes. The sediment is used to prepare oyster nutrition powder. The liquid is filtered with a precision filter. The filtrate passes through a diameter-to-height ratio of 1:20 and a linear flow velocity of 10L / min. The molecular sieve fillin...

Embodiment 2

[0029] Another example of the method for preparing biological zinc from oysters of the present invention comprises the following steps:

[0030] Wash and shell 40kg live oysters, add 1 times the purified water at 5°C, add 0.15w / w% lysozyme with an activity of 18,000 U / g from egg white, and grind it in a colloid mill with a rotation speed of 2500r / min. The fineness is 0.20mm, placed in a bioreactor, adding 0.5w / w% cellulase with an activity of 250,000 U / g, 2.5w / w% activity with a fungal proteolytic enzyme of 40,000 U / g and 0.8w / w% lipase with an activity of 100,000 U / g, reacted at a temperature of 42°C and a speed of 150r / min for 20 hours, then centrifuged at a radius of 800mm and a speed of 3500r / min for 15 minutes, and the sediment was used to prepare oyster nutrition powder , the centrifugate is filtered with a precision filter, the filtrate passes through a diameter-to-height ratio of 1:40, and a linear flow velocity of 25L / min. The molecular sieve filling material is resi...

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PUM

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Abstract

The invention discloses a method for preparing biological zinc from oysters. The method comprises the following steps: removing shells of living oysters, grinding fresh oyster meat by a colloid mill to obtain meat pulp, adding cellulase, fungal proteinase and lipase for enzymolysis, performing fine filtering, separating to obtain filter liquor rich in biological zinc, separating the filter liquor with a molecular sieve, performing vacuum concentration, and performing spray drying to obtain 100-150-mesh biological zinc dry powder. The biological zinc drying powder has the characteristics of being high in water solubility, uniform in molecular weight, high in biological activity, free from activation, direct and fast in absorption, free of fishy smell of the oysters, and the like, and is a good functional factor in healthy products having high additional values.

Description

technical field [0001] The invention relates to a method for preparing biological zinc by using oysters, in particular to a method for preparing biological zinc by using fresh and live oysters through enzymolysis, molecular sieve separation, concentration and spray drying. Background technique [0002] Oyster, commonly known as oyster, sea oyster, oyster yellow, is the largest cultured shellfish in the world and an important marine shellfish in my country. It belongs to the Oyster family and is a bivalve mollusc. waters. Oyster meat is rich in nutrients and is known as "undersea milk". The west calls it "the magic food given by the gods", and Japan calls it "sea の brown rice, the source of the root". According to scientific research and analysis, the protein content in dried oyster meat can reach 45-47w / w%. According to the evaluation of the World Food and Agriculture Organization, the mass ratio and completeness of essential amino acids in oyster meat are better than human ...

Claims

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Application Information

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IPC IPC(8): A23L33/00
Inventor 潘国仕米运宏
Owner GUANGXI PANDA BIOTECH DEV CO LTD
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