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Chlamydia trachomatis and ureaplasma urealyticum nucleic acid detection kit

A technology for Ureaplasma urealyticum and Chlamydia trachomatis, which is applied in the field of Chlamydia trachomatis/Ureaplasma urealyticum nucleic acid detection kits, which can solve the problems of low detection efficiency, low sensitivity, and poor specificity of kits

Inactive Publication Date: 2016-02-24
SANSURE BIOTECH INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0011] The invention provides a Chlamydia trachomatis / Ureaplasma urealyticum nucleic acid detection kit to solve the problems of low detection efficiency, poor specificity and low sensitivity of the kit in the prior art

Method used

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  • Chlamydia trachomatis and ureaplasma urealyticum nucleic acid detection kit
  • Chlamydia trachomatis and ureaplasma urealyticum nucleic acid detection kit

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Embodiment 1

[0037] This embodiment provides a Chlamydia trachomatis / Ureaplasma urealyticum nucleic acid detection kit, the Chlamydia trachomatis / Ureaplasma urealyticum nucleic acid detection kit includes a Chlamydia trachomatis / Ureaplasma urealyticum PCR reaction solution, and the Chlamydia trachomatis The Ureaplasma urealyticum PCR reaction liquid further comprises the following components:

[0038] (1) 0.2 μmol / L-0.4 μmol / L probe for target polynucleotide detection, the probe sequence for target polynucleotide detection is: Chlamydia trachomatis probe: 5'-CTTAAAAGACAATGGATTACCTAT-3 '; Ureaplasma urealyticum probe: 5'-TGGAAGGGGCAAGAGATGGTAAGT-3'.

[0039] (2) 0.2mmol / L deoxyribonucleoside triphosphate.

[0040] (3) Upstream and downstream primers for target polynucleotide amplification of 0.2 μmol / L-0.4 μmol / L, described upstream and downstream primers for target polynucleotide amplification and probes for target polynucleotide detection The needles are primers and probes derived from ...

Embodiment 2

[0052] This embodiment provides the operation steps of the Chlamydia trachomatis / Ureaplasma urealyticum nucleic acid detection kit described in the above-mentioned Example 1 for detecting Chlamydia trachomatis / Ureaplasma urealyticum DNA in unknown samples such as genital secretions:

[0053] 1. Reagent preparation

[0054] According to the number of samples to be tested, negative control, positive control, and positive reference products A-D, take the corresponding amount of PCR reaction solution and enzyme mixture in proportion (reaction solution 40 μl / person + enzyme mixture 2 μl / person), and mix thoroughly. Homogenize PCR-mix, centrifuge briefly and set aside.

[0055] 2. Sample processing

[0056] 1. Method A: Nucleic acid extraction by magnetic bead method

[0057](1) Cracking virus: add 200 μl-1ml DNA extraction solution I to each tube, then add 100 μl-1ml sample to be tested, cover the tube cap, shake and mix for 10 seconds, and centrifuge instantaneously;

[0058] (...

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Abstract

The invention provides a chlamydia trachomatis and ureaplasma urealyticum nucleic acid detection kit which comprises a chlamydia trachomatis and ureaplasma urealyticum PCR reaction solution. The PCR reaction solution comprises a reaction buffer solution, deoxyribonucleoside triphosphote, upstream and downstream primers used for target polynucleotide amplification, a probe used for target polynucleotide detection and the like. The probe used for target polynucleotide detection is high in specificity. By the adoption of the kit, chlamydia trachomatis and ureaplasma urealyticum DNA in an unknown sample like genital secretion can be rapidly detected at the same time, the detection sensitivity can reach 200 copies / ml, the detection range is 200 copies / ml-4.00E+10 copies / ml, the reliable experiment basis is provided for diagnosing chlamydia trachomatis and ureaplasma urealyticum infection, and the problems that an existing kit is low in detection efficiency, poor in specificity and low in sensitivity can be solved.

Description

technical field [0001] The invention relates to a chlamydia trachomatis / ureaplasma urealyticum nucleic acid detection kit, in particular to a fluorescence quantitative PCR chlamydia trachomatis / ureaplasma urealyticum nucleic acid detection kit. Background technique [0002] Chlamydia trachomatis (CT) is a prokaryotic microorganism with a unique developmental cycle and strict intracellular parasitism. It is a special microorganism between viruses, bacteria and Rickettsia. It belongs to the bacterial phylum, Rickettsia, Chlamydiales and Chlamydia. It is one of the most common venereal pathogens at home and abroad, and the genital chlamydia infection ranks third among STDs in my country, and its incidence rate has a tendency to increase year by year. After the body is infected with CT, it can induce type-specific cellular immunity and humoral immunity, but usually the immunity is not strong and lasts for a short time, thus often causing persistent infection, recessive infectio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/04C12R1/01
CPCC12Q1/6851C12Q1/689C12Q2531/113C12Q2537/143C12Q2545/101
Inventor 戴立忠杨帮林李勃邓中平
Owner SANSURE BIOTECH INC
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