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Enzyme method degumming method

A degumming method and enzymatic technology, applied in the direction of fat production, fat oil/fat refining, etc., can solve the problems of low product inhibition reaction efficiency, difficulty in continuous production, long reaction time, etc., to reduce enzyme loss and purification costs, Effects of cost reduction and short separation time

Inactive Publication Date: 2016-02-24
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a high-efficiency method for the problems of high cost, difficulty in continuous production, low reaction efficiency and long reaction time caused by product inhibition in the current process of enzymatic removal and synchronous recovery of phospholipids in oil. Fast enzymatic degumming method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Take an appropriate amount of Ultra phospholipase in a conical flask, add 100 times the volume of water, mix well, take 2g of crude enzyme solution (enzyme concentration 50U / mL), 1.5g of sodium citrate, mix well and add 1.5g poly Ethylene glycol 400 and 2.5g crude soybean oil were put into a stoppered Erlenmeyer flask and mixed evenly. The pH value of the mixed solution was 7.7, and placed on a constant temperature shaker with a rotation speed of 200rpm, controlled at 50°C for 3h. After the reaction is over, set the speed of centrifugation at 4600rpm for 5min and divide it into four layers, which are the upper liquid layer, the steroid layer, the middle liquid layer and the lower liquid layer. phase, and the middle liquid layer is the enzyme-rich phase. In addition, the middle and lower liquid layer was taken to measure its enzyme activity and the phosphorus content of the upper liquid layer. The phosphorus content in the oil phase was only 13.96mg / kg, and the removal r...

Embodiment 2

[0026] Take an appropriate amount of Ultra phospholipase in a conical flask, add 100 times the volume of water, mix well, take 5.2g of crude enzyme solution (enzyme concentration 50U / mL), add 1.6g of sodium citrate, mix well and add 3.2 g[BMIM]BF 4 and 5g of crude soybean oil were mixed evenly in a stoppered Erlenmeyer flask, the pH value of the mixed solution was 7.7, and placed on a constant temperature shaker with a rotation speed of 200rpm, controlled at 50°C for 10 minutes. Take another crude enzyme solution not less than the reaction volume, add 2.5g soybean crude oil, add water to the same volume as the experimental group, and react under the same conditions as a control. After the reaction finishes, set 4600rpm rotating speed centrifugation 5min, and experiment group is divided into four layers, is successively upper liquid layer, steroid layer, middle liquid layer and lower liquid layer, and upper liquid layer product is lipid product phase, and steroid layer is The ...

Embodiment 3

[0028] Take an appropriate amount of Ultra phospholipase in a conical flask, add 100 times the volume of water, mix well, take 2g of crude enzyme solution (enzyme concentration 50U / mL), add 1.5g of sodium citrate, mix well and add 1.5g Polyethylene glycol 400 and 2.5 g of crude soybean oil were put into a stoppered Erlenmeyer flask and mixed evenly. The pH value of the mixed solution was 7.7, and placed on a constant temperature shaker with a rotation speed of 200 rpm, controlled at 50° C. for 1 h. Take another crude enzyme solution not less than the reaction volume, add 2.5g soybean crude oil, add water to the same volume as the experimental group, and react under the same conditions as a control. After the reaction is over, set the speed of centrifugation at 4600rpm for 5min and divide it into four layers, which are the upper liquid layer, the steroid layer, the middle liquid layer and the lower liquid layer. phase, and the middle liquid layer is the enzyme-rich phase. In a...

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Abstract

Provided is an enzyme method degumming method. The method comprises the following steps: firstly, an enzyme solution with a phospholipase concentration of 1-30000U / mL is prepared, soluble salts, a hydrophilic solvent and lipids rich in phosphatide are added in the enzyme solution, a three-liquid-phase system is prepared, and the mass ratios of the soluble salts, the hydrophilic solvent and the lipids to the enzyme solution are 0.1-0.9, 0.1-5 and 0.1-10; secondly, the three-liquid-phase system is placed in a reactor, a shaking-table reaction is carried out at a constant temperature, after the reaction is finished, the mixture is allowed to stand or centrifuged until the mixture is divided into four layers which are an upper liquid layer, a solid-like layer, a middle liquid layer and a lower liquid layer in order from top to bottom, phosphatide is mainly enriched in the solid-like layer, the upper liquid layer product is collected and a lipid product without phosphatide is obtained. The method has advantages of low energy consumption, high raw material utilization rate, mild reaction conditions and the like, and the technical problems are solved that synchronous removal and recovery of phosphatide in oil through present enzyme methods are difficult for industrialization.

Description

technical field [0001] The invention belongs to the field of bioengineering and food technology, relates to the separation and application technology of enzymes, and particularly relates to a method for degumming by enzymatic method. Background technique [0002] In recent years, selective hydrolysis to remove phospholipids from oil has received extensive attention. Since the phospholipids in the oil can cause rancidity and anti-coloring of the oil, more than 45 million tons of soybean oil are processed globally every year through complex processes such as washing, pickling, and enzymatic hydrolysis to reduce the phosphorus content to less than 10 ppm. The phospholipids with various functions after hydrolysis, especially modified phospholipids, are widely used in food, medicine, cosmetics, feed and other fields (more than 200,000 tons). [0003] In many methods of removing and modifying phospholipids, phospholipids in oil are selectively hydrolyzed by phospholipase, so that...

Claims

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Application Information

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IPC IPC(8): C11B3/00
CPCC11B3/003
Inventor 王永华陈华李志刚杨博王卫飞陈华勇莫宗超杨良华
Owner SOUTH CHINA UNIV OF TECH
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