A method for culturing and screening paclitaxel-producing bacteria from Taxus chinensis

Abstract

The invention provides a method of culturing and screening taxol-producing fungus from taxus chinensis, wherein the method includes the following steps: 1) culturing tissue of the taxus chinensis, and performing separation and purification to obtain endophytic fungus; 2) culturing the endophytic fungus in the step 1), preliminary screening a fermentation metabolite through thin layer chromatography to screen the strains being capable of producing taxol or analogues thereof; 3) with cytotoxicity test, determining the inhibition rate of the metabolite to CHO cells; and 4) performing further analysis through high performance liquid chromatography to determine a taxol-producing strain. The method of separating the taxol-producing fungus from taxus chinensis is simple in process and low in cost. The taxol-producing fungus extracted from through the method is greatly increased in content of produced taxol when being used for microorganism cultivation. The method provides a wider approach for microorganism engineering.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for cultivating and screening taxol-producing bacteria from yew. Background technique [0002] Paclitaxel was first isolated from the bark of Taxus breviifolia and named paclitaxel. Paclitaxel was later confirmed to have a unique anti-cancer mechanism. Due to its unique anti-cancer mechanism, it has good curative effect on some advanced tumors that ordinary anti-cancer drugs are helpless, and has outstanding curative effect on a variety of clinical malignant tumors, which is completely different from the previous marketed drugs. any anticancer drug. The U.S. Food and Drug Administration (FDA) officially approved paclitaxel for clinical use in December 1992. [0003] Since Taxus has been listed as a national first-class protected plant as an endangered species, it is obvious that the extraction of paclitaxel from the plant tissue of Taxus has been greatly restricted. Meet ...

AI Technical Summary

Problems solved by technology

[0003] Since Taxus has been listed as a national first-class protected plant as an endangered species, it is obvious that the extraction of paclitaxel from the plant tissue of Taxus has been greatly restricted. Meet the needs of clinical medicine

Taxus cell tissue culture requires strict conditions, low yield and expensive chemical synthesis, so neither of these two ways is suitable for obtaining paclitaxel in large quantities

The production of paclitaxel by microbial fermentation is a method that does not need to rely on yew and has great potential for industrial production. However, the paclitaxel produced by the paclitaxel bacteria screened by the current methods has a low content and cannot meet industrial requirements.

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