Near infrared analysis method of geniposide content

Abstract

Provided is a near infrared analysis method of the geniposide content. A near infrared spectrum is utilized for rapidly, accurately and efficiently analyzing the geniposide content in cape jasmine. The method includes the following steps that the geniposide content in sample cape jasmine is detected through a liquid chromatography method so that a sample model set can be formed; near infrared spectrum scanning is performed on the cape jasmine to be detected; data modeling is performed on the scanned cape jasmine near infrared spectrum through a partial least squares method, and then a modeling set and a validation set are formed; data denoising processing is performed on the modeling set and the validation set through a Savitzky-Golay method; the sample model set, the modeling set and the validation set are input into infrared spectrum analysis equipment, and the geniposide content in the cape jasmine to be detected is calculated based on the sample model set; cross validation is performed on the modeling set and the validation set. The method is easy and convenient to implement, determination is rapid, results are accurate, and direct and nondestructive quantitative discrimination and rapid detection of Chinese medicinal herbs can be achieved.

Description

technical field [0001] The invention relates to a method for analyzing the content of geniposide in gardenia jasminoides by using near-infrared spectroscopy. Background technique [0002] In recent years, the quality of traditional Chinese medicine has been controversial, and there are still quality problems in the Chinese medicine market. Using an analytical technique to identify fake and inferior Chinese medicinal materials is an effective way to improve the current situation of the Chinese medicine market and ensure the safety of people's medicines. [0003] The identification of Chinese herbal medicines is actually a kind of chemical pattern recognition. The chemical composition of Chinese medicines is very complex, which is easy to cause adulteration, mixed origin, etc., and is affected by factors such as geography. Taking gardenia as an example, the content of geniposide in gardenia in different production areas is not the same, and these differences are directly refle...

AI Technical Summary

Problems solved by technology

Taking gardenia as an example, the content of geniposide in gardenia in different production areas is not the same, and these differences are directly reflected in the spectrum of gardenia. Chromatography is the longest and most widely used method in the research of Chinese herbal extracts. , the most mature quantitative analysis technology, but there are some things to be improved in the determination of the index component content in chromatography. For example, HPLC quantitative analysis needs to consume a large amount of organic solvents such as methanol and acetonitrile, and the pretreatment time of samples is long, which is not conducive to rapid detection

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