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A human colon cancer cell line dxh-1 and its application

A technology of colon cancer cells, DXH-1, applied in the field of human colon cancer cell line DXH-1 and its cultivation

Active Publication Date: 2018-08-17
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing human intestinal cancer cell lines and their characteristics are still not fully satisfied with the mechanism of the occurrence and development of colorectal cancer, and more primary intestinal cancer cell lines with different characteristics are still needed to study the occurrence and development mechanism of colorectal cancer. So far, no primary intestinal cancer cell lines cultured directly from intestinal cancer lesions have been reported.

Method used

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  • A human colon cancer cell line dxh-1 and its application
  • A human colon cancer cell line dxh-1 and its application
  • A human colon cancer cell line dxh-1 and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0045] 1. Materials:

[0046] 1. The configuration method of cell culture medium

[0047]1.1 Culture medium, DMEM / F12 complete medium composition is as follows: 10% (v / v) fetal bovine serum (Australian Gibico company), 100U / ml pyruvate, 100U / ml non-essential amino acid, 100U / ml glutamine, 100U / ml penicillin, 100U / ml streptomycin (Hangzhou Jinuo Company), solvent is DMEM / F12 (1:1) culture medium (U.S. Gibico Company);

[0048] 1.2 Digestion solution: RPMI1640 medium containing 1 mg / ml collagenase and 1 mg / ml hyaluronidase IV (Sigma, USA).

[0049] 2. Other cell culture materials

[0050] Trypsin: 0.025% (w / v) trypsin solution; cell cryopreservation solution (fetal calf serum: DMSO=9:1, v / v); PBS buffer.

[0051] 3. Specimen source

[0052] Li Moumou, an 83-year-old male patient from the Oncology Surgery Department of the Second Affiliated Hospital of Zhejiang University School of Medicine, obtained the patient's consent before the operation and signed an informed consent f...

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Abstract

The invention provides a human colon carcinoma cell line DXH-1, which is derived from primary lesion of human sigmoid colon carcinoma, and application of the human colon carcinoma cell line as a human colon carcinoma occurrence, human colon carcinoma development or human colon carcinoma transfer cell model, application of the human colon carcinoma cell line in establishing a human colon carcinoma animal model and application of the human colon carcinoma cell line in researching a human colon carcinoma occurrence mechanism and / or medicines for treating the human colon carcinoma. The human colon carcinoma cell line DXH-1 disclosed by the invention can be stably transferred for more than 50 generations, so that an appropriate material is provided for colorectal carcinoma mechanism and drug screening. In an in-vivo nude mice experiment, the human colon carcinoma cell line shows a relatively strong tumorigenesis cavity, and the cell line, transplanted subcutaneously in nude mice by 1*106 cells, is capable of promoting 100% (5 / 5) tumorigenesis after 35 days; the DXH-1 cell has certain drug resistance to colon carcinoma chemotherapeutics (5FU, such as oxaliplatin, irinotecan and the like), so that a material is provided to the researches on a colon carcinoma chemotherapeutic resistance mechanism; and the human colon carcinoma cell line DXH-1 is preserved in China Center for Type Culture Collection in Wuhan University, Wuhan, China with number of CCTCC No: C201543.

Description

(1) Technical field [0001] The invention relates to a human colon cancer cell line DXH-1 derived from a sigmoid colon cancer lesion, a culture method and application thereof. (2) Background technology [0002] Colorectal cancer is one of the most common malignant tumors in my country, and its incidence rate has continued to increase in recent years. At present, it is recognized that the evolution history of normal intestinal mucosa-adenoma-carcinoma is the evolution process of intestinal cancer, and many genes such as APC, K-Ras, TP53, and C-MYC are involved in the occurrence and development mechanism of intestinal cancer, but the specific mechanism of the occurrence and development of intestinal cancer is currently The mechanism needs further study. [0003] At present, traditional treatment options such as surgery, radiotherapy and chemotherapy are still the main ways to treat colorectal cancer. With the deepening of human research on the occurrence and development of col...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/09A61K35/407A61P35/00C12Q1/02
Inventor 丁克峰胡烨婷肖乾何金杰郑树
Owner ZHEJIANG UNIV
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