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China human pancreatic carcinoma in-situ cell line

A technique for pancreatic cancer and cancer cells, applied in the field of cell lines, to achieve the effects of strong tumorigenicity, enriched cell bank, and stable traits

Active Publication Date: 2018-12-11
郭世伟
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But about a kind of Chinese pancreatic cancer in situ cancer cell line of the present invention, there is no report yet

Method used

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  • China human pancreatic carcinoma in-situ cell line
  • China human pancreatic carcinoma in-situ cell line
  • China human pancreatic carcinoma in-situ cell line

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The establishment of embodiment 1HPPS-1 cell line

[0033] Nude mice: 5, female, weighing 16.3±1.2g, aged 5 weeks, raised in SPF environment.

[0034] A fresh resection specimen of pancreatic cancer in situ (female, 63 years old, pancreatic cancer in situ) was obtained from Shanghai Changhai Hospital, and immediately immersed in pre-cooled sterile HBSS buffer (containing 500 U / ml penicillin G, 500 μg / ml streptomycin sulfate and 1.50 μg / ml amphotericin B). In the biosafety cabinet, wash the specimen with fresh sterile HBSS buffer solution, cut into small pieces of 20-50 mg, and puncture and inoculate subcutaneously in the axillary back of nude mice. After inoculation, the animals were in good health and behaved normally. 40 days after inoculation, small tumor nodules were found under the skin of the inoculation site by touch, and the small nodules began to grow significantly 50 days after inoculation, and the tumor volume exceeded 300mm by 70 days after inoculation 3 ...

Embodiment 2

[0037] The biological characteristic of embodiment 2HPPS-1 cell line

[0038] The invention adopts RPMI 1640 culture medium containing fetal bovine serum and insulin for culture, so that it can grow in vitro for a long time and pass stably. When the cells are passed to more than 20 generations, the cell properties are gradually stabilized, and the relevant biology, genetics and tissue source identification are carried out until the 50th generation has the same stable properties. According to the experimental observation and verification, the cells grown in vitro are epithelial-like. Under the inverted microscope, the cells adhere to the wall and grow evenly, with different sizes and paving stone-like shapes. grow. Genetic studies have confirmed that the cells are aneuploid, with severe chromosome number and structural aberrations, consistent with the genetic characteristics of malignant tumors.

[0039] (1) Morphological observation

[0040] The culture flask of cultured HP...

Embodiment 3

[0047] Embodiment 3HPPS-1 cell line is applied to establish animal model

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Abstract

The invention relates to a China human pancreatic carcinoma in-situ cell line. The China human pancreatic carcinoma in-situ cell line was collected into China Center for Type Culture Collection, and the collection number is CCTCC NO:C201623. The invention also provides application and an establishing method of the China human pancreatic carcinoma in-situ cell line. The China human pancreatic carcinoma in-situ cell line has the advantages that the China human pancreatic carcinoma in-situ cell line is directly established on China human pancreatic carcinoma in-situ, and a cell library of human pancreatic carcinoma is enriched; the tumorigenicity is realized, and is strong after the cell line is inoculated to a bare mouse, and the tissue slices represent that the histology characteristic of transplantation tumor is similar to the histology characteristic of primary tumor; the material is provided for further studying on the generation mechanism of the pancreatic carcinoma, and a platformis provided for screening of medicines for treating the pancreatic carcinoma; the China human pancreatic carcinoma in-situ cell line has stable character, and the stable passage for multiple times isrealized.

Description

technical field [0001] The invention relates to the technical field of cell lines, in particular to a Chinese pancreatic cancer in situ cancer cell line. Background technique [0002] Pancreatic cancer has a very high mortality rate and is the leading cause of death among malignancies. In developed countries, it is the disease with the highest tumor-related mortality rate; in my country, the incidence rate is also increasing year by year. In recent years, with the development of the economy, the improvement of living conditions and the aging of the population, the incidence of pancreatic cancer has continued to rise, and it has become a major disease that affects people's health. [0003] The clinical symptoms of pancreatic cancer often appear late, and early diagnosis is difficult. Due to the anatomical and biological characteristics of the pancreas, it is easy to invade surrounding organs and metastasize. Organs such as spine, kidney, or adrenal gland can only be operate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09C12Q1/02
CPCG01N33/5011C12N5/0693C12N2500/02C12N2501/734Y02A50/30
Inventor 郭世伟
Owner 郭世伟
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