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Human hepatoma carcinoma cell line and application thereof

A technology for liver cancer cells and cells, applied in the field of cell lines, can solve the problems of low biodiversity and large differences in biological properties of liver cancer, and achieve the effect of stable properties

Active Publication Date: 2015-04-08
ZHONGSHAN HOSPITAL FUDAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is to provide a new human liver cancer cell line and its preparation aiming at the existing human liver cancer cell line which has low biodiversity and is quite different from the clinical biological characteristics of liver cancer. method and use

Method used

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  • Human hepatoma carcinoma cell line and application thereof
  • Human hepatoma carcinoma cell line and application thereof
  • Human hepatoma carcinoma cell line and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Preparation of LIXC-002 cells

[0041]Nude mice: 5 nude mice, male, weighing 16.0±1.0 g, aged 5 weeks, raised in SPF environment. Nude mice were provided by Shanghai Slack Experimental Animal Technology Co., Ltd.

[0042] A fresh resection specimen of clinical liver cancer was obtained from Shanghai Zhongshan Hospital (male, 71 years old, primary liver cancer, the pathological diagnosis result was: (right lobe of liver) hepatocellular carcinoma, grade II differentiation, nodular cirrhosis of surrounding liver tissue, pulse Tumor thrombi were seen in the tube; hepatitis B two-and-a-half tests showed: HBs antigen positive, HBe antibody positive, HBc antibody positive), immediately immersed in pre-cooled sterile HBSS buffer (containing 500U / ml penicillin G, 500μg / ml streptomycin sulfate and 1.25 μg / ml amphotericin B). In the biosafety cabinet, wash the specimen with fresh sterile HBSS buffer solution, cut into small pieces of 20-50 mg, and puncture and inoculat...

Embodiment 2

[0046] Example 2 Biological characteristics and application of LIXC-002 cells

[0047] The invention adopts RPMI 1640 culture solution containing fetal bovine serum and insulin to cultivate LIXC-002 cells, so as to enable long-term growth and stable passage in vitro. When the cells are passed to more than 20 generations, the cell properties are gradually stabilized, and the relevant biology, genetics and tissue source identification are carried out until the 50th generation has the same stable properties. Through experimental observation and verification, LIXC-002 cells grown in vitro have a typical epithelial-like morphology, lose contact growth inhibition, and show malignant growth. Genetic studies have confirmed that the cells are aneuploid, with severe chromosome number and structural aberrations, consistent with the genetic characteristics of malignant tumors. The cell genome has HBV DNA integration. The LIXC-002 cells can form tumors in nude mice and have tumorigenicit...

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Abstract

The invention provides a human hepatoma carcinoma cell and application thereof. The human hepatoma carcinoma cell is preserved in China Center of Typical Culture and Preservation, and the preservation number of the human hepatoma carcinoma cell is CCTCC No. C201072. The human hepatoma carcinoma cell can be used for producing human liver cancer in mammal to prepare the human liver cancer model which can be used for further selecting the candidate medicine for treating the human liver cancer. The human hepatoma carcinoma cell line has stable properties and can be transferred stably for multiple times. The properties can be maintained stable from 20th generation to 50th generation of the passages. The human hepatoma carcinoma cell line has the biological properties of the human liver cancer on the clinic, hepatitis B DNA is integrated in the cancer cell genetic group, so novel experimental materials which are more close to the biological properties of the clinic liver cancer can be provided for researching the liver cancer.

Description

technical field [0001] The invention belongs to the field of cell lines, in particular to a human liver cancer cell line and application thereof. Background technique [0002] Primary liver cancer is one of the common malignant tumors of the digestive tract. There are about 1 million new cases of liver cancer every year in the world. It is the sixth most common cancer in the world and the third leading cause of cancer death in the world. The incidence of primary liver cancer has obvious regional characteristics. my country is a country with a high incidence of the disease. Its incidence rate accounts for 55% of the world, and its mortality rate accounts for 45% of the world. At present, about 320,000 cases of liver cancer patients die every year. In my country, primary liver cancer ranks second in the incidence of malignant tumors. In recent years, it has gradually increased. It has the characteristics of high morbidity, high mortality and low cure rate. There is a lack of e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/09C12N5/10C12Q1/02
Inventor 樊嘉秦宵然周俭胡刚代智闻丹忆张亚华
Owner ZHONGSHAN HOSPITAL FUDAN UNIV
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