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Establishing method for dendrobe characteristic fingerprint spectrum

A technology of characteristic fingerprints and establishment methods, which is applied in measuring devices, instruments, scientific instruments, etc., can solve problems such as one-sided evaluation methods, and achieve good stability, simple pre-processing methods, and good reproducibility

Active Publication Date: 2016-01-20
北京蓝标一成科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the quality control of Dendrobium in my country is mainly limited to the quality control of individual or certain types of ingredients. The evaluation method is relatively one-sided, and it is not enough to systematically and completely indicate the overall quality of Dendrobium medicinal materials. It is urgent to explore more advanced and effective quality control. means

Method used

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  • Establishing method for dendrobe characteristic fingerprint spectrum
  • Establishing method for dendrobe characteristic fingerprint spectrum
  • Establishing method for dendrobe characteristic fingerprint spectrum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1: the establishment of characteristic fingerprint of Dendrobium officinale

[0045] (1) Preparation of the test solution (i.e. standard solution): take the dried sample of Dendrobium candidum, pulverize it with a pulverizer, pass through a pharmacopoeia sieve (aperture 0.335mm), accurately weigh 1.000g of Dendrobium candidum powder, and place it in a 100ml conical In the bottle, add 50mL of 75% methanol (V water: V methanol = 25:75), take it out after ultrasonication for 30min at room temperature, filter, and concentrate the filtrate to dryness with 75% methanol solvent (V water: V methanol = 25: 75) Dissolve, and finally transfer to a 5ml volumetric flask to constant volume, shake well, and filter with a 0.45 μm microporous membrane to obtain a standard solution.

[0046] (2) Preparation of the reference substance solution: Accurately weigh 0.164 mg of the reference substance Schaftoside, dissolve and dilute it with 75% methanol (V water: V methanol = 25:75...

Embodiment 2

[0054] Embodiment 2: the establishment of characteristic fingerprint of Dendrobium nobile

[0055] (1) Preparation of the test solution (i.e. standard solution): take the dried sample of Dendrobium nobile, pulverize it with a pulverizer, pass through a pharmacopoeia sieve (aperture 0.335mm), accurately weigh 1.000g of Dendrobium nobile powder, and place it in 100ml In the Erlenmeyer flask, add 50mL of 75% methanol (V water: V methanol = 25:75), take it out after ultrasonication for 30 min at room temperature, filter, and concentrate the filtrate to dryness with 75% methanol solvent (V water: V methanol = 25:75), and finally transferred to a 5ml volumetric flask to constant volume, shaken up, and filtered through a 0.45μm microporous membrane to obtain a standard solution.

[0056] Steps (2)~(4) are the same as shown in Example 1, just obtain the fingerprint collection of Dendrobium nobile, Figure 4 It is the HPLC fingerprint of Dendrobium officinale.

[0057] (5) According ...

Embodiment 3

[0060] Example 3: The establishment of the characteristic fingerprint of Dendrobium dendrobii

[0061] (1) Preparation of the test solution (i.e. standard solution): get the dried sample of Dendrobium dendrobii, pulverize it with a pulverizer, pass through a pharmacopoeia sieve (aperture 0.335mm), accurately weigh 1.000g of Dendrobium dendrobium powder, and place it in 100ml In the Erlenmeyer flask, add 50mL of 75% methanol (V water: V methanol = 25:75), take it out after ultrasonication for 30 min at room temperature, filter, and concentrate the filtrate to dryness with 75% methanol solvent (V water: V methanol = 25:75), and finally transferred to a 5ml volumetric flask to constant volume, shaken up, and filtered through a 0.45μm microporous membrane to obtain a standard solution.

[0062] Steps (2)~(4) are as shown in embodiment 1, just obtain the fingerprint collection of dendrobium dendrobium, Figure 6 It is the HPLC fingerprint of Dendrobium dendrobium.

[0063] (5) Ac...

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Abstract

The invention relates to an establishing method for a dendrobe characteristic fingerprint spectrum. The establishing method comprises the following steps: (1) preparing a standard sample liquid: taking dendrobe, smashing, sieving, adding methyl alcohol, ultrasonically filtering, rotating and steaming the filtrate, concentrating till being dry, dissolving with a methyl alcohol solvent, finally keeping constant volume and shaking up to prepare the standard sample liquid; (2) preparing a reference substance liquid: precisely weighting a certain amount of isoschaftoside and diluting with a methyl alcohol solution to prepare the reference substance liquid; (3) measuring a standard fingerprint spectrum: respectively injecting the standard sample solution and the reference substance solution into a high performance liquid chromatograph, and calculating the relative retention time and the retention peak area of each characteristic peak of a standard sample by taking isoschaftoside chromatographic peak as a reference peak of the fingerprint spectrum to prepare the standard fingerprint spectrum. According to the method for establishing the fingerprint spectrum, provided by the invention, the nature of the fingerprint chromatographic peak with the reference peak area being more than 5% is determined, excessive fuzziness of the existing fingerprint spectrum is broken through, the main ingredients of the fingerprint spectrum are further determined and the integrality and ingredient specificity of the dendrobe characteristic fingerprint spectrum are unified.

Description

technical field [0001] The invention relates to a method for establishing a fingerprint in the field of traditional Chinese medicine, in particular to a method for establishing a dendrobium characteristic fingerprint. Background technique [0002] Dendrobium is a commonly used tonic Chinese medicine, mainly for the Dendrobium genus. Dendrobium is the largest genus of Orchidaceae, including Dendrobium candidum, Dendrobium nobile, Dendrobium dendrobii, Dendrobium drumstick and other varieties. There are more than 1,100 species of Dendrobium in the world, of which 76 are found in my country. Dendrobium has a long history of medicinal use. As early as in ancient times, Dendrobium was listed as a nourishing top grade in "Shen Nong's Materia Medica". For a long time, with the development of the times, Dendrobium has been regarded as a precious Chinese herbal medicine with very important nourishing effects. Dendrobium is clinically used to treat a variety of diseases, and has ph...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 梁琼麟邵自星梁晓萍赵田
Owner 北京蓝标一成科技有限公司
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