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DPB strain and application of DPB strain to domestic sewage treatment

A technology for denitrifying phosphorus accumulating bacteria and domestic sewage is applied in the field of environmental biology, which can solve the problem of low denitrification and phosphorus removal efficiency, and achieve the effects of improving treatment efficiency and stability, strong phosphorus accumulation ability, and good decontamination ability.

Inactive Publication Date: 2015-12-09
CHINA UNIV OF GEOSCIENCES (WUHAN)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Pei Haiyan et al. screened a strain of Pseudomonas pseudoalcaligenes CL-1 in the patent of "a denitrifying phosphorus-accumulating bacteria with dual ability of nitrogen and phosphorus removal and its application". The nitrogen and phosphorus removal rate of the bacteria in the first 24 hours Only 56.93% and 43.56% respectively, the efficiency of nitrogen and phosphorus removal is not high

Method used

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  • DPB strain and application of DPB strain to domestic sewage treatment
  • DPB strain and application of DPB strain to domestic sewage treatment
  • DPB strain and application of DPB strain to domestic sewage treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: Isolation, screening and identification of bacterial strains

[0021] (1) Material preparation:

[0022] 1. From a well-run A 2 Activated sludge is collected from the aeration tank of the / O process as a source for screening denitrifying phosphorus-accumulating bacteria, and inoculated on-site into a sterilized high-concentration nitrogen-phosphorus medium.

[0023] 2. Medium:

[0024] High concentration nitrogen and phosphorus medium: glucose 10g / L, CaCl 2 0.2g / L, MgSO 4 0.5g / L, (NH4) 2 SO 4 2g / L, KH 2 PO 4 0.25g / L, KNO 3 1g / L, adjust the pH to 7.0.

[0025] LB liquid medium is: peptone 10.0g, yeast extract 5.0g, NaCl 10.0g, distilled water 1000mL, pH 7.0.

[0026] LB solid medium is: peptone 10.0g, yeast extract 5.0g, NaCl 10.0g, agar powder 15g, distilled water 1000mL, pH7.0.

[0027] 3. Experimental instruments and equipment:

[0028] Jerell SHZ-82A air bath constant temperature oscillator,

[0029] Heated Incubators,

[0030] Sanyo autom...

Embodiment 2

[0044] Application of Example 2 Pseudomonas aeruginosa LLHD-1 in domestic sewage treatment

[0045] The ability of Pseudomonas aeruginosa LLHD-1 to treat simulated domestic sewage was investigated, and the degradation ability of Pseudomonas aeruginosa LLHD-1 to COD, total nitrogen and total phosphorus in simulated wastewater was studied. The components of simulated domestic sewage are: KNO 3 0.6g / L; KH 2 PO 4 1g / L; MgSO 4 ·7H 2 O1g / L; sodium succinate 2.4g / L; pH 7.2; high temperature and high pressure sterilization at 121.5°C for 20 minutes. Take 600mL of simulated domestic sewage and add it to a 1L Erlenmeyer flask, add 6mL of Pseudomonas aeruginosa LLHD-1 bacterial suspension, culture on a shaker at 30°C and 120 rpm for 2 days, take samples every 12 hours, and measure the CODcr in the solution, Total nitrogen, total phosphorus content, calculation of the removal rate of each index, the removal rate of the three indicators like Figure 4 shown. The results showed tha...

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Abstract

The invention belongs to the technical field of environmental biology, and particularly relates to a DPB (denitrifying phosphorus removing bacterium) strain and application of the DPB strain to domestic sewage treatment. DPB provided by the invention is pseudomonas aeruginosa LLHD-1, which is preserved in CCTCC (China Center for Type Culture Collection) on May 27, 2015, and the preservation number is CCTCC NO:M2015331. The pseudomonas aeruginosa LLHD-1 has good decontamination capability on sewage; the removal rate on CODcr can reach 90.7 percent; the removal rate on total nitrogen can reach 93.7 percent; and the removal rate of phosphorus reaches 51 percent.

Description

technical field [0001] The invention belongs to the technical field of environmental biology, and in particular relates to a denitrifying phosphorus-accumulating bacterium and its application in domestic sewage treatment. Background technique [0002] With the continuous improvement of human living standards and the rapid development of industrial production, the problem of water pollution is becoming more and more serious. Nitrogen and phosphorus are biological nutrient elements. Exceeding the standard of nitrogen and phosphorus will cause eutrophication of the water body and destroy the habitat of the water body. The main lakes in my country are heavily polluted with nitrogen and phosphorus, and the eutrophication problems caused by this are prominent. Although physical and chemical methods can achieve good phosphorus removal effects, the construction cost is high, the technology is complicated, and the operation cost is high; in addition, chemical phosphorus removal is a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/385
Inventor 李平王艳红姜舟戴欣月张睿洪文涛
Owner CHINA UNIV OF GEOSCIENCES (WUHAN)
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