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Gastrodia elata polypeptide, preparation method and medical application of gastrodia elata polypeptide in antibiosis and antiviral

A technology of Gastrodia elata and protein, applied in the direction of antiviral agents, medical preparations containing active ingredients, antibacterial drugs, etc., can solve the problems of unseen Gastrodia elata polypeptides, etc., to promote skin wound healing, non-irritant, skin permeability Good results

Active Publication Date: 2015-11-25
通化仁生源生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

After searching, there is no relevant literature report on Gastrodia elata polypeptide

Method used

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  • Gastrodia elata polypeptide, preparation method and medical application of gastrodia elata polypeptide in antibiosis and antiviral
  • Gastrodia elata polypeptide, preparation method and medical application of gastrodia elata polypeptide in antibiosis and antiviral

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Embodiment 1

[0021] 2) After filtration under reduced pressure, concentrate the obtained gastrodia protein extract to a concentration of the original drug of 1 (g / ml);

[0022] 3) Add 0.4% compound enzyme hydrolyzate to the concentrated solution by volume ratio. The compound enzyme hydrolyzate contains 1.5% papain, 0.8% trypsin, and 0.6% flavor protease. The enzymolysis temperature is 55°C and the enzymolysis time is After 40 minutes, pass the enzymatic solution through a 10KDa membrane for ultrafiltration, centrifuge at 8000 r.p.m for 20 minutes, and obtain the filtrate under the membrane as the Gastrodia elata polypeptide extract, which is stored at 4°C for future use.

[0023] 4) SDS-PAGE electrophoresis method was used to measure the molecular weight of Gastrodia elata polypeptide, and the ultra-low molecular weight protein marker of Suleibao Company was used as the standard. The standard protein was composed of 3 kinds of polypeptides and 2 kinds of low-molecular-weight proteins, and t...

Embodiment 2

[0026] 2) After filtration under reduced pressure, the obtained Gastrodia elata protein extract was concentrated to a concentration of the original drug of 0.8 g / mL;

[0027] 3) Add 0.2% compound enzyme hydrolyzate to the concentrated solution by volume ratio. The compound enzyme hydrolyzate contains 2% papain, 0.6% trypsin, and 0.5% flavor protease. The enzymolysis temperature is 50°C and the enzymolysis time is 30min, then pass the enzymolysis solution through a 10KDa membrane for ultrafiltration, centrifuge at 8000r.p.m for 20min, the obtained filtrate is the Gastrodia elata polypeptide extract, and store it at 4°C for later use;

[0028] 4) SDS-PAGE electrophoresis method was used to measure the molecular weight of Gastrodia elata polypeptide, and the ultra-low molecular weight protein marker of Suleibao Company was used as the standard. The standard protein was composed of 3 kinds of polypeptides and 2 kinds of low-molecular-weight proteins, and the molecular weight range ...

Embodiment 3

[0031] 2) After filtration under reduced pressure, the obtained Gastrodia elata protein extract is concentrated to a concentration of the original drug of 0.5 g / mL;

[0032] 3) Add 0.8% compound enzyme hydrolyzate to the concentrated solution by volume ratio. The compound enzyme hydrolyzate contains 3% papain, 1.5% trypsin, and 1% flavor protease. The enzymolysis temperature is 60°C and the enzymolysis time is 1.5h, then pass the enzymatic solution through a 10KDa membrane for ultrafiltration, centrifuge at 8000r.p.m for 20min, the obtained filtrate is the Gastrodia elata polypeptide extract, and store it at 4°C for later use;

[0033] 4) SDS-PAGE electrophoresis method was used to measure the molecular weight of Gastrodia elata polypeptide, and the ultra-low molecular weight protein marker of Suleibao Company was used as the standard. The standard protein was composed of 3 kinds of polypeptides and 2 kinds of low-molecular-weight proteins, and the molecular weight range was 3....

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Abstract

The invention provides gastrodia elata polypeptide, a preparation method and medical application of the gastrodia elata polypeptide in antibiosis and antiviral. Gastrodia elata protein is extracted, filtered and concentrated, is subjected to enzymolysis, ultrafiltration and separation, is mixed with suitable auxiliary materials according to use and application fields in proportion, and then is prepared into corresponding preparations. The gastrodia elata polypeptide is a product obtained from natural resources, and even a small amount of gastrodia elata polypeptide (10-7mol / L) has obvious biological activity. An experiment proves that the gastrodia elata polypeptide has excellent antibiosis and antiviral capabilities, has quite high killability on common pathogenic bacteria and viruses, also has the advantages of zero hemolytic activity and zero plasma coagulation activity, and can be used for preparing medicines which have an antibiosis function and can resist viral influenza, viral hepatitis, influenzavirus A sous-type H1N1, coronavirus and human acquired immunodeficiency viruses.

Description

technical field [0001] The invention provides a gastrodia elata polypeptide and a preparation method thereof. The invention further discloses the medical use of the gastrodia elata polypeptide in antibacterial and antiviral, which belongs to the technical field of medical biopharmaceuticals. Background technique [0002] Gastrodia elata, as a kind of traditional Chinese medicine, has the functions of relieving wind and convulsions, sedative hypnosis, analgesia, anti-platelet aggregation, anti-oxidation, anti-aging, anti-hypertensive, anti-convulsions, anti-epilepsy, improving learning and memory, enhancing non-specific immunity and cellular immunity, etc. Efficacy, however, at present domestic and foreign studies on Gastrodia elata are mostly focused on the extraction, separation, purification and pharmacological activity of Gastrodia elata polysaccharides, gastrodin, alkaloids and other active ingredients. After searching, there is no relevant literature report on Gastrodia...

Claims

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Application Information

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IPC IPC(8): C12P21/06A61K38/01A61P31/04A61P31/10A61P31/12A61K36/8988
CPCY02A50/30
Inventor 逯家辉孟庆繁滕利荣程瑛琨王贞佐王迪谢晶滕乐生刘艳王雪李臣亮陈彦羽李剑光
Owner 通化仁生源生物科技股份有限公司
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