Solid-state fermentation medium of tobacco endophytic fungus strain ycef005 and its cultivation method

Abstract

The invention belongs to the field of microbial fermentation and provides a medium for fermentation cultivation, especially solid fermentation cultivation, of endophytic fungi, and a cultivation method of the endophytic fungi. The solid fermentation medium comprises a substrate and additional nutrient compositions as well as water, wherein based on mass percentage, the substrate comprises 60% of bran, 10% of soybean cake powder, 10% of rice bran and 10% of corn broken grains; the content of water is 50% of total mass of the substrate; based on 1kg of total mass of the substrate, the additional nutrient compositions comprise 12.96g of sucrose, 4.00g of ammonium nitrate, 12.70g of peptone, 1.50g of monopotassium phosphate, 10.00g of calcium sulfate and 0.48g of magnesium sulfate; and the weight of distilled water is 500g. According to the invention, solid fermentation of tobacco endophytic fungi is carried out and initiated for the first time, an artificial neural network of YCEF005 type tobacco endophytic fungi is obtained for the first time, and the fermented product of the tobacco endophytic fungi is fast and effectively obtained.

Description

technical field [0001] The invention belongs to the field of microbial fermentation and provides a culture medium for endophytic fungus fermentation culture, especially solid fermentation culture and a culture method thereof. Background technique [0002] Solid-state fermentation (SSF) refers to a biological reaction process with one or more microorganisms in a solid substrate with a certain humidity in the presence of no or almost no free water (Singhania et al., 2009) . From the perspective of the biological reaction process, solid-state fermentation is a biological reaction process in which the gas phase is the continuous phase. More specifically, we can consider solid-state fermentation to be a fermentation method that is used when the medium is solid particles. (Mitchell et al., 2000b). Compared with liquid fermentation, solid fermentation has many significant advantages: cheaper medium, lower energy consumption, lower input costs, easier control of fermentation volum...

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Problems solved by technology

Taking the influence of fermentation time (fermentation process) on solid fermentation as an example, Biazus and others found that when the corn maltose was used for research, the amount of product was very low at the beginning of fermentation, and then the amount of product rose rapidly until it reached the maximum value, after reaching the maximum product amount, the formation of the product is inhibited, and the activity of the product will also decrease (Biazus et al., 2006)

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