Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Fungus solid fermentation medium

A solid fermentation, culture medium technology, applied in fermentation, microorganism-based methods, microorganisms, etc., can solve problems such as product activity decline

Active Publication Date: 2015-03-25
ZHEJIANG UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Taking the influence of fermentation time (fermentation process) on solid fermentation as an example, Biazus et al. found that when the fermentation started with corn maltose, the amount of product was very low, and then the amount of product rose rapidly until it reached the maximum value, after reaching the maximum product amount, the formation of the product is inhibited, and the activity of the product will also decrease (Biazus et al., 2006)

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fungus solid fermentation medium
  • Fungus solid fermentation medium
  • Fungus solid fermentation medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1: the determination of solid fermentation medium water content

[0042] In solid fermentation, the water content of the medium will affect the growth of microorganisms. If the water content is too high, agglomerates will be formed during the cultivation process, thereby affecting the progress of fermentation. In this embodiment, the water content of the culture medium is changed by changing the ratio of material to water between raw millet and potato juice and the amount of potato juice in the nutrient solution in the preparation of cooked millet.

[0043] According to the above design theory, on the basis of a large number of preliminary selection of medium, the following three medium formulations were selected for experiments to determine the optimal water content in the medium.

[0044] The solid fermentation medium of formula 1 consists of the following ingredients: 100g of cooked millet, 20g of perlite, and 25ml of nutrient solution.

[0045] The prepa...

Embodiment 2

[0071] Embodiment 2: the optimization of solid fermentation medium formula

[0072] The medium used is a solid fermentation medium with a determined water content, and its formula is as in formula three in Example 1.

[0073] The optimization of the medium adopts an orthogonal design. As shown in Table 1, the amount of glucose is factor one, the amount of peptone is factor two, the amount of low-temperature defatted soybean powder is factor three, and the amount of potassium dihydrogen phosphate is factor four. An orthogonal design with 4 factors and 3 levels was carried out. The factors and levels, and the orthogonal design table are shown in Table 2:

[0074] Table 1: Orthogonal design table with 4 factors and 3 levels (unit: gram):

[0075] level\factor

glucose

Peptone

Low temperature defatted soybean flour

Potassium dihydrogen phosphate

level 1

0.50

1.00

1.00

0.05

level 2

3.00

4.00

4.00

0.30

l...

Embodiment 3

[0093] Embodiment 3, optimum pH of nutrient solution in solid fermentation medium

[0094] In the solid fermentation medium, glucose, low-temperature defatted soybean powder, peptone, potassium dihydrogen phosphate and magnesium sulfate are dissolved in potato juice to prepare the nutrient solution part of the medium, and the nutrient solution will be mixed into cooked millet and perlite In the granular system composed of it, it becomes an important part of the culture medium. A single factor test was performed on the initial pH of the nutrient solution.

[0095] In this example, NaOH solution (5 mol / L) and hydrochloric acid solution (analytical grade hydrochloric acid and pure water in a volume ratio of 1:1) were used to adjust the pH of the nutrient solution. Set 7 groups of different pH treatments, namely 4, 5, 5.5, 6, 6.5, 7, and 8. Three parallel experiments were carried out for each treatment group, and each group was controlled by the blank medium not inoculated with ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

The invention belongs to the field of microbial fermentation, and provides a medium for fungus fermentation culture and particularly for solid fermentation culture. Specifically, the invention discloses a fungus solid fermentation medium which consists of the following components: 100g of cooked millet, 19-21g of perlite and 24-26ml of nutrient solution, wherein a preparation method of the cooked millet comprises the following steps of: adding 55-65ml of tomato juice into 100g of raw millet, and heating in a sterilization pot at 120-122 DEG C for 14-16 minutes to obtain the cooked millet; and a preparation method of the nutrient solution comprises the following steps of: dissolving 0.5-3g of glucose, 5-7g of defatted soybean powder, 1-5g of peptone, 0.3-0.55g of monopotassium phosphate and 0.15g of magnesium sulfate in 25-50ml of tomato juice, and adjusting the pH to 6 to obtain the nutrient solution. By adopting the medium, a product of aerogenesis fungus solid fermentation can be quickly and effectively obtained.

Description

technical field [0001] The invention belongs to the field of microbial fermentation and provides a medium for fungal fermentation culture, especially solid fermentation culture. Background technique [0002] Solid-state fermentation (SSF) refers to a biological reaction process with one or more microorganisms in a solid substrate with a certain humidity in the presence of no or almost no free water (Singhania et al., 2009 ). From the perspective of the biological reaction process, solid-state fermentation is a biological reaction process in which the gas phase is the continuous phase. More specifically, we can consider solid-state fermentation to be a fermentation method that is used when the medium is solid particles. (Mitchell et al., 2000b). Compared with liquid fermentation, solid fermentation has many significant advantages: cheaper medium, lower energy consumption, lower input costs, easier control of fermentation volume, less waste water (Pandey A et al., 2000; Pand...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12P1/02C12R1/645
Inventor 冯晓晓刘诚章初龙林福呈
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com