Synthesis of phosphorescent iridium complex and application of phosphorescent iridium complex for fluorescence labeling of schistosome cercaria

Abstract

The invention belongs to the field of photobiology labeling for preventing and treating parasitic diseases, and relates to synthesis of a phosphorescent iridium complex and the purpose of the phosphorescent iridium complex for the fluorescence labeling of schistosome cercaria. The invention aims to realize the synthesis of the phosphorescent complex and perform fluorescence labeling on cercaria. The iridium complex is in the structure that two azotic aromatic compounds as bidentate ligands are matched with an aromatic compound containing dinitrogen in a coordination manner so as to form the iridium complex. The fluorescence labeling method of the cercaria comprises the following steps of: (1) obtaining the fresh cercaria; (2) dissolving the phosphorescent complex with dimethyl sulfoxide so as to accurately compound 1 mmol of a mother solution, and then diluting the mother solution with a PBS buffer solution so as to obtain 5muM of a diluent; (3) performing fluorescence staining at room temperature; and (4) collecting phosphorescence signals for fluorescent imaging. Through design and synthesis, the phosphorescent iridium complex is used as a fluorescent probe to be used for fluorescence labeling of the schistosome cercaria, and the problems of autofluorescence interference and photobleaching in the fluorescent imaging process of the cercaria are successfully solved.

Description

technical field [0001] The invention belongs to the field of bio-optical labeling for the prevention and treatment of parasitic diseases. More specifically, the invention relates to the synthesis of a phosphorescent iridium complex and its use in fluorescent labeling of schistosome cercariae. The invention provides a novel fluorescent labeling method for schistosome cercariae , the phosphorescent iridium complex has the beneficial effect of fluorescently labeling the cercariae of the schistosome. Background technique [0002] Among the global human parasitic diseases, schistosomiasis is the second largest tropical parasitic disease after malaria. Schistosomiasis is endemic in 74 countries and regions. It is estimated that 652 million people are currently at risk, with 193 million infected persons and about 120 million symptomatic cases, of which 20 million are severe cases. my country is the main endemic area of ​​schistosomiasis. Schistosomiasis is a typical water-borne zo...

AI Technical Summary

Problems solved by technology

However, the use of organic small-molecule fluorescent dyes as fluorescent probes has the following disadvantages: (1) The excitation and emission of organic fluorescent dyes are located in the ultraviolet or visible light region, which is prone to tissue photodamage and autofluorescence of biological tissues during fluorescence imaging, and interferes The quality of fluorescence imaging; (2) The photobleaching resistance of organic fluorescent dyes is poor. When they are irradiated by strong excitation light and the irradiation time increases, photobleaching is prone to occur, and the fluorescence intensity decreases significantly, resulting in too weak signals for fluorescence imaging. check out

How to achieve effective fluorescent labeling of cercariae with fluorescent dyes requires a systematic study of the structure-activity relationship between the fluorescent labeling of cercariae and the structure of the phosphorescent complex. At the same time, the phosphorescent complex needs to meet the characteristics of high luminous efficiency, good photostability and good biocompatibility , such a labeling method is different from the traditional fluorescent labeling of cells, and the phosphorescent complex labeling of cercariae has more influencing factors and technical difficulties

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