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Method for preparing biological composite artificial trachea and application thereof

An artificial trachea and biological technology, applied in the field of medical devices, to achieve the effect of avoiding rejection, avoiding dependence and reducing complications

Active Publication Date: 2015-11-18
SHANGHAI PULMONARY HOSPITAL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the limitations of current equipment development and the particularity of the structure and function of the trachea, there is currently no mature and practical trachea bioprinting solution.

Method used

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  • Method for preparing biological composite artificial trachea and application thereof
  • Method for preparing biological composite artificial trachea and application thereof
  • Method for preparing biological composite artificial trachea and application thereof

Examples

Experimental program
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preparation example Construction

[0040] The invention provides a method for preparing a biological composite artificial trachea, comprising the following steps:

[0041] Step 1, using a bronchoscope, aseptically brush the bronchial epithelial cells, culture and expand in vitro; extract auricle chondrocytes, digest, disperse, and culture and expand in vitro;

[0042] Step 2, mix collagen and hyaluronic acid with deionized water, filter and sterilize through a bacteria filter to obtain soft materials; after fumigation of chitosan with ethanol, irradiate with ultraviolet light for complete sterilization, and evenly disperse in the sterile in culture medium;

[0043] Step 3, mixing the soft material obtained in step 2 with the culture medium at a volume ratio of 1:1 to make a soft material mixture for resuspending cells;

[0044] Step 4, dissolving the polylactic acid-glycolic acid copolymer and polyethylene glycol in dichloromethane, stirring ultrasonically to make the dispersion uniform, and placing it in a fu...

Embodiment 1

[0051] Using a bronchoscope, the bronchial epithelial cells were collected from sterile transplant recipients by brushing, cultured and expanded in vitro; auricular cartilage was extracted by surgery, digested and dispersed, and cultured and expanded in vitro.

[0052] Mix 10% collagen and 5% hyaluronic acid with deionized water according to mass-volume ratio, filter and sterilize through a bacteria filter to obtain a soft material. After the chitosan is fumigated with ethanol, it is irradiated under an ultraviolet lamp for 24 hours to completely sterilize it, and is dispersed in the culture solution at a mass volume ratio of 8%.

[0053] Mix the above two solutions 1:1 to make a soft material mixture for resuspending cells.

[0054] The two kinds of cells amplified in vitro were respectively digested, resuspended, counted and centrifuged, the supernatant was discarded, and resuspended in the aforementioned soft material mixture at a cell concentration of 1 million / ml to make ...

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Abstract

The invention provides a biological artificial trachea and a preparation method thereof in combination with a 3D printing technology. Materials adopted for the biological artificial trachea simultaneously have flexibility and a certain mechanical strength, so that physical and mechanical properties of the trachea serving as a hollow organ in the chest are met. The artificial trachea is porous and loosened in structure and favorable for nutrient diffusion and ingrowth of autologous vessels. In addition, seed cells obtained by expansion of airway epithelial cells and cartilage cells of a receptor are printed on the trachea wall, and patients' rejection reaction to cells is avoided. Besides, the seed cells are composed of mature cells in terminal differentiation, the induced differentiation problem and the tumor formation risk caused by the use of stem cells can be avoided. The spatial distribution of the cells is accurately controllable in printing, and the restorative process is accelerated according to the histology regular distribution. Finally, a trachea support can be degraded in vivo, complications caused by the fact that foreign matter remains in vivo are reduced, and the risk of taking out the foreign matter through a second operation is avoided.

Description

technical field [0001] The invention relates to the field of medical devices, in particular to a preparation method and application of a biological composite artificial trachea. Background technique [0002] For malignant tumors of the trachea, benign space-occupying lesions, or tracheal stenosis, or trauma, after long-segment tracheectomy (more than 50% of the total length in adults, and more than 30% in children), the resection margins cannot be directly anastomosed, and a tracheal substitute is required. The anastomosis can be performed only if it is inserted, and the breathing and ventilation function of the trachea can be restored. According to Neville et al., an ideal trachea replacement material needs to have the following characteristics: (1) sealed lumen structure; (2) flexible texture; (3) good biocompatibility; (4) low inflammatory response ; (5) low immunity; (6) contribute to the growth of tracheal epithelial tube lining. With the deepening of researchers' res...

Claims

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Application Information

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IPC IPC(8): A61L27/38A61L27/26A61L27/56A61L27/58A61F2/04B33Y10/00B33Y80/00
Inventor 李牧章文俊张洪波陈昶殷瑞雪郑卉邢天龙王龙
Owner SHANGHAI PULMONARY HOSPITAL
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