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Water-insoluble natural melanin extraction method

A natural melanin and extraction method technology, applied in natural dyes, chemical instruments and methods, azo dyes, etc., can solve the problem of undefined melanin, and achieve the effect of large-scale extraction, high process repeatability and high yield

Active Publication Date: 2015-11-04
YANCHENG TEACHERS UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since current techniques such as biochemistry and biophysics cannot provide a chemical structural formula for such biomacromolecules, there is no strict definition of melanin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1) Select dry, mildew-free sunflower seed shells with black seed coats, peel off the sunflower seed shells, and crush them into 60 meshes;

[0024] 2) Mix it with 0.5mol / L NaOH at a weight-to-volume ratio of 1:15 (g / ml), bathe in 70°C for 2 hours, cool to room temperature, and centrifuge at 20,000rpm for 20 minutes. The supernatant is the crude melanin extract;

[0025] 3) Use 1mo / L HCl to adjust the pH value of the crude melanin extract in step 2) to 2.0, precipitate the melanin, leave it at room temperature for 2 hours, and centrifuge to separate the obtained precipitate into a crude melanin extract;

[0026] 4) Suspend the crude melanin extract obtained in step 3) in 6 mol / L HCl with a weight-to-volume ratio of 1:10 (g / ml), put it in a boiling water bath for 2 hours, cool to room temperature, centrifuge at 10,000 rpm for 30 minutes, and remove the supernatant, Collect the precipitated melanin solid particles;

[0027] 5) Wash the solid particles obtained in step 4) ...

Embodiment 2

[0032] 1) Select dry, mildew-free sunflower seed shells with black seed coats, peel off the sunflower seed shells, and crush them into 60 meshes;

[0033] 2) Mix it with 0.1mol / L NaOH at a weight-to-volume ratio of 1:15 (g / ml), bathe in water at 60°C for 3 hours, cool to room temperature, and centrifuge at 10,000rpm for 30 minutes. The supernatant is the crude melanin extract;

[0034] 3) Use 1mo / L HCl to adjust the pH value of the crude melanin extract in step 2) to 2.0, precipitate the melanin, leave it at room temperature for 2 hours, and centrifuge to separate the obtained precipitate into a crude melanin extract;

[0035] 4) Suspend the crude melanin extract obtained in step 3) in 6mol / L HCl with a weight-to-volume ratio of 1:15 (g / ml), put it in a boiling water bath for 2 hours, cool to room temperature, centrifuge at 10,000 rpm for 30 minutes, and remove the supernatant, Collect the precipitated melanin solid particles;

[0036] 5) Wash the solid particles obtained in ...

Embodiment 3

[0041] 1) Select dry, mildew-free sunflower seed shells with black seed coats, peel off the sunflower seed shells, and crush them into 40 meshes;

[0042] 2) Mix it with 0.3mol / L NaOH solution at a weight-to-volume ratio of 1:20 (g / ml), put it in a water bath at 60°C for 2 hours, cool to room temperature, and centrifuge at 10,000rpm for 20 minutes, and the supernatant is the crude melanin extract;

[0043] 3) Use 1mo / L HCl to adjust the pH value of the crude melanin extract in step 2) to 2.5, precipitate the melanin, leave it at room temperature for 6 hours, and centrifuge to separate the obtained precipitate into a crude melanin extract;

[0044] 4) Suspend the crude melanin extract obtained in step 3) in 6 mol / L HCl with a weight-to-volume ratio of 1:10 (g / ml), place in a boiling water bath for 2 hours, cool to room temperature, centrifuge at 10,000 rpm for 10 minutes, and remove the supernatant, Collect the precipitated melanin solid particles;

[0045] 5) Wash the solid p...

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Abstract

The invention relates to the field of water-insoluble melanin development and utilization, in particular to a method for extracting water-insoluble melanin from animals, plants and microorganisms. The method includes the steps that firstly, dried, mildew-free and homogenized sunflower seed shells are selected and smashed, the smashed sunflower seed shells are mixed with 0.1-2.0 mol / L of NaOH according to the weight-to-volume ratio of 1:10-1:20, water bath is conducted on the mixture for 2.0-10.0 hours at the temperature of 40 DEG C to 80 DEG C, cooling and centrifuging are conducted, and a liquid melanin crude extract is obtained; the PH value is adjusted to range from 1.5 to 3.0, standing and sedimentation are conducted, and a melanin crude extract is obtained; impurities are removed through the steps of conducting boiling water bath hydrolysis for 2.0 hours to 6.0 hours in 5.0-7.0 mol / L of HCl, conducting repeated washing through an organic solvent, conducting re-dissolving and the like, then vacuum free-drying is conducted, and dried pure melanin powder is obtained. The water-insoluble melanin extracted through the method has the advantages of being high in purity, high in yield and the like.

Description

technical field [0001] The invention belongs to the technical field of melanin extraction, separation and processing, and is suitable for the production and processing of water-insoluble melanin from plant sources, microorganism sources and most animal sources, and is particularly suitable for the preparation method of water-insoluble melanin. Background technique [0002] Melanin is a general term for a family of phenolic or indole biomacromolecular pigments with complex and diverse structures. In nature, it widely exists in the biosphere, lithosphere, hydrosphere, atmosphere and interstellar space. In the biosphere, melanin is distributed in animals, higher plants and microorganisms, but it is not a black pigment, but also red, orange, yellow, green, blue and other colors of melanin. Since current techniques such as biochemistry and biophysics cannot provide a chemical structural formula for such biomacromolecules, there is no strict definition of melanin. Melanins are d...

Claims

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Application Information

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IPC IPC(8): C09B61/00C09B67/54
CPCC09B61/00C09B67/0096
Inventor 李超峰唐伯平周春霖葛宝明
Owner YANCHENG TEACHERS UNIV
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