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Phellinus identification method

An identification method and a technology of a measurement method, which are applied in the identification field of Phellinus, can solve problems such as misuse of Phellinus, damage to food research and development, etc., and achieve the effect of enhancing transparency and facilitating observation

Inactive Publication Date: 2015-10-28
JIANGSU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the process of using Phellinus Phellinus, if different kinds of Phellinus Phellinus are misused or abused, this will damage the next research and development of food

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Phellinus Phellinus Appearance Appraisal

[0033] Remove impurities from the harvested Phellinus, remove the bad wood, soil or culture substrate attached to the base of the fruiting body, clean it or cut Phellinus into small pieces, let it dry naturally or put it on the Dry in an oven at 40-50°C. Take the original drug, remove impurities, wash it, cut it into small pieces, and dry it in the shade or at 40-50°C. Or take small pieces and remove impurities. For example, the ventral surface of the cap has a pore-like structure, and the surface is golden yellow or brownish yellow; the pore is oval or polygonal, and the flesh of the cap is yellow, brown or glossy wood color, and the color near the cap surface becomes darker. Bacterial pores can be seen on the cross-section of the fruiting body, the powder is yellow or brownish yellow, the hyphae are visible, the spores are oval, dispersed in the mycelial mass, and have a slightly sour smell and slightly bitter. I...

Embodiment 2

[0034] Example 2 Phellinus microscopic identification

[0035] Microscopic identification of Phellinus mycelium: take out the Phellinus mycelium, observe it under an optical microscope, take pictures, and record the experimental results

[0036] Microscopic observation of Phellinus powder: Grind Phellinus into powder, pass through a 200-mesh sieve, take an appropriate amount of powder on the center of the glass slide, drop 1-2 drops of chloral hydrate, and stir well; place the slide on the outer flame of an alcohol lamp, and heat it Stir while stirring, remove from heat when boiling, do not burn dry, burn, repeat 2-5 times depending on the condition of the glass slide, add 1-2 drops of glycerin after a little cooling, stir well, cover with a cover slip, absorb excess glycerin , observed under an optical microscope, and then photographed and recorded data. The function of chloral hydrate is to expand the cells, facilitate the observation of tissues, cells and their contents, a...

Embodiment 3

[0038] Component Identification of Phellinus Phellinus of Embodiment 3

[0039] Moisture determination: Take 2-5g of Phellinus japonica samples, lay flat in a flat weighing bottle, and the height of the flat laying should be less than or equal to 5cm. Loose samples should be less than or equal to 1cm for accurate measurement. Unscrew the bottle cap and dry it at 100-105°C. After 5 hours, re-tighten the bottle cap and place it in a desiccator for 30 minutes. Weigh it accurately, then dry it at 100-105°C for 1 hour, let it cool down, and then weigh it. Know that the difference in the weight range of the last two weighings is within 5mg, and calculate it from the lost weight. How much water is the test product. See Table 1 for results as determined by moisture limits.

[0040] Determination of ash content: Grind the Phellinus sample so that it can pass through the No. 2 sieve, mix it evenly, weigh 2-3g of the Phellinus sample, place it in a crucible and burn it until the final w...

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Abstract

The invention discloses a phellinus identification method. The method comprises the steps of firstly, grinding phellinus into powder, conducting sieving with a 200-mesh screen, taking and placing a defined amount of powder in the center of a glass slide, dripping 1-2 drops of chloral hydrate, and conducting the stirring till the mixture becomes uniform; placing the glass slide over the outer flame of an alcolhol burner, conducting stirring while the heating is conducted, moving the glass slide away when the mixture gets boiling, preventing the mixture from being dried out or burnt, and repeating the process based on the glass slide conditions; after the glass slide gets cooler, adding in 1-2 drops of glycerin, conducting stirring till the mixture becomes uniform, covering the mixture with cover glass, absorbing away excessive glycerin, and conducting observation under a microscope, wherein if hypha is visible and in yellow or claybank, the hypha is provided with multiple branches, the branch diameters range from 1.9 micrometers to 4.2 micrometers, hypha transverse septa are visible, no clamp connection exists, each phellinus spore is in an oval shape and in faint yellow, the spore diameters range from 2.2 micrometers to 4.0 micrometers, the phellinus spores are dispersed in hypha clusters, no structures such as starch granules or microcrystal exist within the view field, and it can be judged that the hypha is phellinus.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for identifying Phellinus phellidis. Background technique [0002] Sang Huang (Inonotus Sang Huang), also known as Sang Er, Sang Chen, is named after parasitic on mulberry trees. As a precious medicinal fungus, Phellinus has the functions of improving the body's immunity, anti-tumor, resisting cell mutation, anti-bacterial, inhibiting liver fibrosis, stopping bleeding, promoting blood circulation and lowering blood sugar, inhibiting bacteria and reducing inflammation, and treating cancer and hepatitis. A large number of scientific studies have found that Phellinus has the effect of inhibiting the growth of cancer cells, and the anti-cancer rate is as high as 96.7%. It is currently internationally recognized as the most effective medicinal fungus in the field of biological anti-cancer. Phellinus is known as "forest gold" because of its extremely high health benefits. [000...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/84G01N5/04G01N21/79
Inventor 闻燕徐安捷张攀杰
Owner JIANGSU UNIV OF SCI & TECH
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