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Chitosan nanoparticle suspension preparation method and application thereof

A chitosan nanoparticle and chitosan nanotechnology are applied in the fields of blood diseases, liquid transportation, and pharmaceutical formulations. It can solve the problems of poor stability of nanoparticles, complicated operation steps, and large particle size of nanoparticles to achieve stability. Good, optimized preparation parameters, good biocompatibility

Active Publication Date: 2015-10-28
NANJING XINTONG RUIYI MEDICINE SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The above-mentioned preparation methods have the following disadvantages: the prepared nanoparticles have a large particle size, which is prone to sedimentation and aggregation after being placed for a long time, and the stability of the nanoparticles is poor; the operation steps are relatively complicated, and the reproducibility from small laboratory tests to mass production is poor. The organic solvents, cross-linking agents, and surfactants used in the process will remain in the prepared chitosan particles, etc.

Method used

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  • Chitosan nanoparticle suspension preparation method and application thereof
  • Chitosan nanoparticle suspension preparation method and application thereof
  • Chitosan nanoparticle suspension preparation method and application thereof

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preparation example Construction

[0043] The invention provides a kind of preparation method of chitosan nano particle suspension, comprises the steps:

[0044] (1) dissolving chitosan in an aqueous solution of 0.1% to 2.0% by weight of glacial acetic acid to prepare chitosan acetic acid solution; %; The chitosan is one or a combination of carboxymethyl chitosan or modified chitosan;

[0045] (2) Under the condition of stirring, add dropwise sodium tripolyphosphate aqueous solution with a weight percentage of 0.1-1%, the weight ratio of sodium tripolyphosphate and chitosan is 1:2-8, and the dropping rate is 1-2ml / min, continue stirring for 40-100 min to obtain a coarse suspension of chitosan nanometers;

[0046] (3) Filtrating the chitosan nano coarse suspension, performing high-pressure homogenization treatment, the pressure is 500-800 Mpa, and the number of circulation homogenization is 5-15 times, to obtain the chitosan nano particle suspension.

[0047] Add polymer excipients, the polymer excipients are...

Embodiment 1

[0059] Weigh an appropriate amount of chitosan powder and dissolve it in 1% by weight glacial acetic acid solution to prepare chitosan acetic acid solution. Under stirring, the sodium tripolyphosphate solution that is 0.2% by weight percentage is slowly added dropwise in the chitosan acetic acid solution, and the rate of addition is 1~2ml / min, and the weight ratio of sodium tripolyphosphate and chitosan is 1:5. Stirring was continued for 60 min to obtain a coarse suspension of chitosan nanometers. Take the chitosan nano coarse suspension, put it into a high-pressure homogenizer, the pressure is 500Mpa, repeat the homogenization 10 times, collect and obtain the chitosan nano particle suspension with a weight percent content of 0.5%.

[0060] The electron microscopic picture of the chitosan nanoparticle relevant to the present invention is shown in accompanying drawing. Chitosan nanoparticles prepared by ion gel method IGT method (×2.0k) as figure 2 As shown, the appearance ...

Embodiment 2

[0062] Weigh an appropriate amount of chitosan powder and dissolve it in 0.1% by weight glacial acetic acid solution to prepare chitosan acetic acid solution. Under stirring, the weight percentage is that 1% sodium tripolyphosphate solution is slowly added dropwise in the chitosan acetic acid solution, the dropping speed is 1~2ml / min, the weight ratio of sodium tripolyphosphate and chitosan is 1 :8. Continue to stir for 40min to obtain the chitosan nano coarse suspension. Get chitosan nano coarse suspension, insert in high-pressure homogenizer, pressure is 500Mpa homogenization 5 times, and pressure is 800Mpa homogenization 5 times, the collection obtains the chitosan nano particle suspension that weight percentage content is 1% .

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Abstract

The present invention discloses a chitosan nanoparticle suspension preparation method and application thereof. The chitosan nanoparticle suspension preparation method comprises the following steps of: (1) dissolving a chitosan into a glacial acetic acid aqueous solution to prepare a chitosan aqueous acetic acid; (2) under stirring, adding a tripolyphosphate sodium aqueous solution dropwise to obtain a coarse chitosan nano suspension; and (3) after filtering the coarse chitosan nano suspension, processing homogeneously the filtered suspension with high pressure to obtain a nano chitosan suspension with a uniform particle size distribution. The invention has the advantages of simple operation, good scale-up preparation reproducibility, and low production cost, and is applicable to industrial production. The prepared nanoparticles are of a uniform morphology, small in particle diameter and applicable to preparing products with functions of antibiosis, hemostasis and promoting tissue repair.

Description

technical field [0001] The invention relates to the field of polymer polysaccharides, in particular to a preparation method and application of chitosan nanoparticle suspension. Background technique [0002] As a natural positively charged polysaccharide, chitosan has the characteristics of good biocompatibility, good mucous membrane adsorption and in vivo degradability. For a long time, the main application of chitosan in the field of medicine is as a drug carrier material. Chitosan drug-loaded microspheres can control the release of drugs, and realize the functions of sustained release, controlled release and targeted release of drugs. Improve drug stability and reduce drug toxicity. [0003] In recent years, a large number of studies have proved that chitosan has biological activities such as hemostasis, antibacterial, promotion of tissue repair, anti-tumor, blood fat reduction, immune regulation, anti-aging, anti-oxidation, moisturizing, moisturizing, skin care, hair ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/05A61K9/70A61K9/00A61K9/06A61K9/12A61K9/08A61K9/10A61K9/107A61K9/02A61L27/20A61L15/28A61P31/04A61P7/04A61P35/00A61P3/06A61P37/02A61P39/06A61P17/16A61Q5/12
Inventor 杨星昊孙健杨天倚
Owner NANJING XINTONG RUIYI MEDICINE SCI & TECH
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