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oral composition

A composition and oral cavity technology, which can be used in oral care, drug combination, cosmetic preparations, etc., can solve the problem of no bactericidal effect, etc., and achieve the effect of excellent cell damage inhibition effect.

Inactive Publication Date: 2017-09-01
LION CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, it is difficult to say that human defensins have a sufficient bactericidal effect on periodontal pathogenic bacteria, so improving the bactericidal power has become a problem
[0006] On the other hand, with regard to the inhibition of cell damage in the oral cavity, it is known that ascorbic acid esters such as ascorbyl phosphate or salts thereof can suppress inflammation while protecting biological tissues from oxidative stress damage by eliminating excess active oxygen generated in the body, and have Damage-inhibiting effect on cells in the oral cavity, but there is still room for improvement in the cell-injury inhibiting effect
In addition, ascorbic acid ester or its salt has no bactericidal effect on periodontal pathogenic bacteria

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0051] Hereinafter, the present invention will be described in more detail by way of Examples, Comparative Examples, and Prescription Examples, but the present invention is not limited to the following Examples. In addition, in the following examples, % represents mass % unless otherwise specified.

[0052] [Example, comparative example]

[0053] A sample solution having the composition shown in Table 1 was prepared by a normal method, and the cell damage inhibitory effect and the oral bacteria sterilizing effect were evaluated by the method shown in the following experimental example. The results are recorded together in the table.

experiment example 1

[0054] Cell damage inhibitory effect test

[0055] Commercially available gingival fibroblast Gin-1 (manufactured by DS Pharmaceutical Biomedical Co., Ltd.) was incubated in Dullbecco's Modified Eagle's Medium (D-MEM) containing 10% fetal bovine serum (FBS) at 37°C, 5% CO 2 pre-cultured under conditions. Prepare 400 μL to make 5 × 10 4 Gin-1 cells / ml were seeded in 48-well plates and further cultured for 24 hours. After removing the culture medium, 400 μL of the sample solutions shown in the table (diluted 20 times with D-MEM containing 10% FBS) were added, respectively, and treated with drugs for 48 hours.

[0056] After the treatment was completed, 1 mM hydrogen peroxide aqueous solution (assumed to be active oxygen) was added for 60 minutes. After the treatment, the sample solution was removed, and 400 μL of a solution containing 1 mM hydrogen peroxide (D-MEM containing 10% FBS) was added, and treated for 60 minutes. After the solution was removed, 200 μL of a cell vi...

experiment example 2

[0064] Bactericidal effect test on bacteria in the oral cavity

[0065] Todd-Hewitt broth (Becton Dickinson, Japan) supplemented with hemin (manufactured by Wako Pure Chemical Industries, Ltd., 500 μg / mL) and menadione (manufactured by Wako Pure Chemical Industries, Ltd., 100 μg / mL) was added with physiological saline. Porphyromonas gingivalis strain ATCC33277 pre-cultured in the 9 cells / mL of bacterial suspension. Mix the bacterial solution with the sample solution shown in the table at a ratio of 1:1 (volume ratio), and let stand for 3 minutes. Then, perform four dilutions with physiological saline, each dilution is 10 times, and smear on the blood agar plate medium with a spiral inoculator. As a control, a mixture of physiological saline and bacterial solution mixed at 1:1 (volume ratio) was used instead of the drug.

[0066] The blood plate medium was cultured at 37° C. under anaerobic conditions (95 vol % nitrogen, 5 vol % carbon dioxide) for 5 days, and the number of...

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Abstract

Provided is an oral composition comprising (A) human defensin and (B) ascorbic acid ester or a salt thereof, which has a bactericidal effect on bacteria in the oral cavity, particularly Porphyromonas gingivalis which is a periodontal pathogenic bacterium and oral cavity It has an excellent inhibitory effect on cell damage and can effectively prevent or treat periodontal disease.

Description

technical field [0001] The present invention relates to an oral composition containing a human defensin, which is excellent in the bactericidal effect against bacteria in the oral cavity, particularly Porphyromonas gingivalis, which is a periodontal pathogen, and the effect of inhibiting cell damage in the oral cavity, and can Effective prevention and treatment of periodontal disease. Background technique [0002] Periodontal disease is an infection caused by bacteria such as Porphyromonas gingivalis (P.gingivalis) and other anaerobic Gram-negative bacteria. Exotoxins (leukotoxins, etc.) etc.) Induce inflammation and damage tissue. On the other hand, in the living body, neutrophils or lymphocytes infiltrate into the periodontal pocket or gingival tissue, and at the same time phagocytize bacteria, and generate an immune response to eliminate these foreign substances by producing specific antibodies. It is pointed out that the reactive oxygen species generated excessively du...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/64A61K8/67A61K31/375A61K38/00A61P1/02A61P31/04A61Q11/00
CPCA61K8/64A61K8/676A61K38/17A61P1/02A61P31/04A61Q11/00A61Q17/005
Inventor 阿部真里近泽贵士铃木苗穗
Owner LION CORP
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