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Myocardial tissue sensor and myocardial tissue chip preparation method

A tissue chip and sensor technology, applied in instruments, scientific instruments, material inspection products, etc., can solve problems such as affecting the detection accuracy, difficult to ensure the coupling between cells and devices, and improve the acquisition accuracy, meet the accuracy requirements, and the size is accurate and controllable. Effect

Inactive Publication Date: 2015-09-23
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

On the other hand, when designing a cell sensor system, biocompatibility is an aspect that must be considered. To test the physiological activity of cells, the cells and the surface of the device must be well coupled, that is, the cells can easily move on the surface of the device. Adhesive growth, while the traditional cell planting technology is difficult to ensure a good coupling between cells and devices, which greatly affects the detection accuracy

Method used

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  • Myocardial tissue sensor and myocardial tissue chip preparation method
  • Myocardial tissue sensor and myocardial tissue chip preparation method
  • Myocardial tissue sensor and myocardial tissue chip preparation method

Examples

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preparation example Construction

[0028] Figure 4 It is a partial schematic diagram of the manufacturing process of myocardial tissue chip. The preparation method of myocardial tissue chip includes the following steps:

[0029] 1) Paste a layer of tape 402 on the surface of a sterilized glass sheet 401;

[0030] 2) Use a laser forming machine to cut at least 5 square contours on the tape along the first cutting track 403. Each square contour is 6-8mm in length and 0.6-1mm in width, separated by 0.1-0.3mm, and moved Except for the cut square tape 404, such as Figure 4 As shown in (a);

[0031] 3) Prepare a poly-N-isopropylacrylamide solution with a mass volume concentration of 10% to 50%, and apply it evenly on the side of the glass sheet with adhesive by spin coating. After curing, remove the adhesive, leaving a square Poly-N-isopropylacrylamide layer 405, such as Figure 4 As shown in (b);

[0032] 4) After heating and melting the polydimethylsiloxane, apply it to the glass sheet by spin coating. After curing, use...

Embodiment 1

[0036] Example 1: Test the effect of cardiac glycoside drugs on myocardium:

[0037] 1) Select or make a square glass sheet with a cross-sectional size of 20×12mm and a thickness of 1mm, and paste a layer of tape on the surface after sterilizing in 75% alcohol for one hour;

[0038] 2) Use a laser forming machine to cut out 13 square contours with a length of 7mm, a width of 1mm, and an interval of 0.1mm on the tape as the first cutting track, and remove the cut square tape;

[0039] 3) Mix the poly-N-isopropylacrylamide solution with a mass volume concentration of 20% with 99% butanol in a volume ratio of 1:1 to make a 10% poly-N-isopropylacrylamide solution. Coating the poly-N-isopropylacrylamide solution on the side glass sheet containing the tape, and remove the remaining tape after curing. At this time, 13 pieces of 7×1mm square poly-N-isopropylacrylamide are left on the glass sheet Floor;

[0040] 4) Mix the polydimethylsiloxane solution and the curing agent at a mass ratio of ...

Embodiment 2

[0045] Example 2: Testing the therapeutic effect of compound Danshen tablets on diseased myocardium:

[0046] 1) Select or make a square glass sheet with a cross-sectional size of 20×12mm and a thickness of 1mm, and paste a layer of tape on the surface after sterilizing in 75% alcohol for one hour;

[0047] 2) Use a laser forming machine to cut 15 square contours with a length of 6mm, a width of 0.6mm, and a mutual interval of 0.2mm on the tape as the first cutting track, and remove the cut square tape;

[0048] 3) Mix the poly-N-isopropylacrylamide solution with a mass volume concentration of 40% with 99% butanol in a volume ratio of 1:1 to make a 20% poly-N-isopropylacrylamide solution, and spin-coat Coating the poly-N-isopropylacrylamide solution on the side glass sheet containing the tape, and remove the remaining tape after curing. At this time, 15 pieces of 6×0.6mm square poly-N-isopropyl propylene are left on the glass sheet Amide layer

[0049] 4) Mix the polydimethylsiloxane...

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Abstract

The invention discloses a preparation method of myocardial tissue sensor and a myocardial tissue chip, and belongs to the technical fields of tissue engineering, biomedicine engineering and sensor. The myocardial tissue sensor comprises a myocardial tissue chip and auxiliary parts, wherein the auxiliary parts include an upper die, a lower die, an upper electrode, a lower electrode, a sensitive plate, a parallel light source and a data acquisition system. According to the invention, myocardial cells are sequentially printed on a glass sheet coated with N-isopropylacrylamide and polydimethylsiloxane by virtue of a cell printing technology so as to prepare the myocardial tissue chip; the myocardial tissue chip is kept in the auxiliary parts and is subjected to pulsatile cultivation to form myocardial tissues consistent in beating rhythm, and then the myocardial tissues are kept in a culture solution containing a medicine and undergo electrical stimulation; and a contractile change signal is collected to evaluate the effect of the medicine on cardiac muscle, so as to achieve the purposes of drug screening, testing and the like. The myocardial tissue sensor disclosed by the invention has the advantages of simple device, wide scope of testing medicine, easy detection and processing of signal, and the like.

Description

Technical field [0001] The invention belongs to the cross field of tissue engineering, biomedical engineering and sensing technology, and particularly relates to a myocardial tissue sensor and a preparation method of a myocardial tissue chip. Background technique [0002] Cardiomyocytes are the main constituent cells of myocardial tissue, which are mainly distributed on the heart wall and large blood vessels near the heart. Cardiomyocytes are short cylindrical fibers with obvious directionality. Compared with cells such as kidney cells and liver cells, which are mainly metabolic functions, the main function of cardiomyocytes is to contract and relax, so as to realize the pumping function of the heart to promote blood circulation. Injury and pathological changes of myocardial tissue can lead to various heart diseases and myocardial diseases, which have high morbidity and mortality, and seriously threaten human survival and quality of life. According to statistics, in the United S...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50
CPCG01N33/5008
Inventor 王小红周新伟
Owner TSINGHUA UNIV
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