A kind of Aspergillus niger and its application in the production of isomaltooligosaccharide

A technology of isomaltooligosaccharide and Aspergillus niger, which is applied in the field of fermentation engineering, can solve the problems of complicated production process and increased production cost, and achieve the effects of simple and easy cultivation method, increased content and shortened conversion reaction time

Active Publication Date: 2018-05-15
GUANGXI NANNING ZHITIAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to obtain a higher purity isomaltooligosaccharide product, it is necessary to use chromatographic separation and other methods for secondary separation on this basis to remove glucose and maltose from IMO-500 syrup to form IMO-900 type products, and its total oligomeric The isomaltose content is about 90%, and the effective trisaccharide content is still only about 45%, which still cannot meet the needs of the high-end market. In addition, the chromatographic separation operation also complicates the production process and greatly increases the production cost

Method used

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  • A kind of Aspergillus niger and its application in the production of isomaltooligosaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: Aspergillus niger is cultivated and its application 1

[0032] (1) Strain culture:

[0033] Seed medium: cornstarch 50g / L, corn steep liquor dry powder 20g / L, pH 4.5.

[0034] Fermentation medium: corn starch 60g / L, corn steep liquor dry powder 30g / L, pH 4.5.

[0035] Aspergillus niger (Aspergillus niger) M1 was used as the fermentation strain, and seed culture was carried out under the condition of 32°C, the liquid volume of the medium was 10%, the rotation speed of the shaker was 200rpm, and the seed age was 12h; The seeds are inoculated in the fermentation medium, the liquid content of the medium is 20%, the rotating speed of the shaker is 200 rpm, and after fermentation at 32° C. for 36 hours, the fermentation broth is vacuum filtered to collect mycelia.

[0036] (2) Strain application

[0037] Apply free mycelium to transform and produce isomaltooligosaccharides, put commercially available maltose syrup with a solid content of 30% in the reactor, a...

Embodiment 2

[0038] Embodiment 2: Aspergillus niger is cultivated and its application 2

[0039] (1) Strain culture:

[0040] Same as the bacterial classification cultivation of embodiment 1.

[0041] (2) Strain application

[0042] Apply free mycelium to transform and produce isomaltooligosaccharides, put commercially available maltose syrup with a solid content of 30% in the reactor, and add free mycelium according to the wet weight of the thallus at 10% (w / v) , under the condition of 60°C, after conversion for 6 hours, the isomaltooligosaccharide syrup was obtained; the effective trisaccharide (IG+P+IG3) content was 68.35% as determined by high performance liquid chromatography.

Embodiment 3

[0043] Embodiment 3: Aspergillus niger is cultivated and its application 3

[0044] (1) Strain culture:

[0045] Same as the bacterial classification cultivation of embodiment 1.

[0046] (2) Strain application

[0047] Immobilized cells are used for transformation to produce isomaltooligosaccharide, and free mycelium of Aspergillus niger (Aspergillus niger) M1 is immobilized with calcium alginate with a mass concentration of 3%. Put the cassava starch saccharification solution with a solid mass percentage of 20% in the reactor, add immobilized cells at 20% (w / v), and convert it for 8 hours at 50°C to obtain isomaltooligosaccharide syrup Measured by high performance liquid chromatography, the effective trisaccharide (IG+P+IG3) content is 45.72%.

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Abstract

The invention discloses an Aspergillus niger, which is classified as Aspergillus niger (Aspergillus niger) M1, the preservation number CCTCC NO: M2014421, and the preservation date is September 23, 2014. The preservation unit: China Center for Type Culture Collection, the Aspergillus niger The α-glucosidase produced by the (Aspergillus niger) M1 strain is an intracellular enzyme, which can produce isomaltooligosaccharides with an effective trisaccharide (IG+P+IG3) content of more than 65% at a high temperature of 55-65 °C, while The reaction time required to prepare IMO‑500 type isomaltooligosaccharide with an effective trisaccharide content of 35% is only half of the reaction time for preparing isomaltooligosaccharide with an effective trisaccharide content of more than 65%. The α-glucosidase produced by the bacterial strain of the present invention has the advantages of excellent temperature characteristics, short glucoside conversion time, high effective trisaccharide content of the product, and the like.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering, and in particular relates to an Aspergillus niger with high alpha-glucosidase production and a method for using it to convert starch saccharification liquid or maltose syrup to prepare isomaltooligosaccharide. Background technique [0002] Isomaltooligosaccharides (IMO) is currently the most widely used and most demanded oligosaccharide functional sweetener. It has special physiological functions such as low calorie, anti-caries, and promoting the proliferation of intestinal bifidobacteria. It has been widely used in food, medicine and health products, feed and other industries. It is a mixture of a series of glucans with a degree of polymerization of 2-10, all of which have at least one α-(1,6) glycosidic bond in their molecules, and the most abundant component is isomaltose (Isomaltose) , IG), panose (Panose, P) and isomaltotriose (Isomaltotriose, IG3), these three components ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P19/14C12R1/685
Inventor 梁智群陈桂光李玮曾伟
Owner GUANGXI NANNING ZHITIAN BIOTECH
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