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Freeze-drying protective agent for hog cholera live vaccine (spleen and lymph tissue origin)

A technology of freeze-dried protective agent and swine fever spleen leaching source, which is applied in the direction of antiviral agents, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., can solve the problem that the effect of live vaccines is not improved, and the immune effect is not good. Ideal, uneven quality and other problems, to achieve the effect of strengthening the ability of B cells to produce and secrete antibodies, improving the body's immunity, and improving the effect of use

Inactive Publication Date: 2015-09-02
浙江美保龙生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the market believes that the immunization effect of the live vaccine of classical swine fever spleen and lymph is not ideal, and there are still misunderstandings about the live vaccine of classical swine fever and spleen. Excessive stress and uneven quality
[0006] In recent years, people have done a lot of research on live vaccine freeze-dried protective agents, most of which are heat-resistant protective agents, which solve the heat-resistant problem of vaccines during transportation and use, but there is little improvement in improving the effect of live vaccines

Method used

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  • Freeze-drying protective agent for hog cholera live vaccine (spleen and lymph tissue origin)
  • Freeze-drying protective agent for hog cholera live vaccine (spleen and lymph tissue origin)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: the preparation of Chinese medicine polysaccharide and Chinese medicine flavone

[0030] a. According to 30 parts of weeping pot grass, 20 parts of golden fruit olive, 20 parts of chicken vine, 30 parts of buffalo horn, 30 parts of mole cricket, 10 parts of fennel, 10 parts of Fangji, 10 parts of lobelia and 10 parts of Passepartout Chinese medicine raw materials, chopped, washed, soaked in cold water overnight, then added purified water 15 times the weight of raw materials, water bathed to 90 ° C, and kept at 90 ° C, boiled for 2 hours, stirring every 10 minutes during the boiling process;

[0031] b. Discard the dregs of the traditional Chinese medicine in a, collect the medicinal liquid, and centrifuge at 10,000 rpm for 10 minutes after cooling at room temperature, discard the precipitate, and collect the supernatant;

[0032] c. The supernatant in b is subjected to alcohol precipitation, the precipitation is the crude Chinese medicine polysaccharide, a...

Embodiment 2

[0038] Embodiment 2: the preparation of bursalin

[0039] a. Take healthy bursa tissue, remove the fascia and adipose tissue from the bursa tissue, and wash it with sterilized cold PBS at pH 7.2.

[0040] b. Weigh the bursa of Fabricius, add PH7.2 sterilized cold PBS at a ratio of 1:1 (W:V), and perform high-speed homogenization in a tissue homogenizer.

[0041] c. Add 2.5% trypsin to the homogenate, freeze and thaw three times, centrifuge at 12000rpm for 20min, and discard the precipitate.

[0042] d. The supernatant is ultrafiltered with an ultrafiltration membrane with a molecular weight cut-off of 1000da, and the liquid under the membrane is the crude extract of bursalin.

[0043] e. The crude extract in d is sterilized by filtration through a 0.22um filter membrane, and then freeze-dried in a vacuum to make bursalin freeze-dried powder.

Embodiment 3

[0044] Embodiment 3: the preparation of a kind of lyoprotectant for swine fever splenic gonorrhea live vaccine

[0045] Add 60g of traditional Chinese medicine polysaccharide, 40g of traditional Chinese medicine flavonoid and 12mg of bursalin to every 1000ml of phosphate buffer solution to obtain the freeze-dried protective agent for live vaccine of swine fever spleen and gonorrhea. Among them, the polysaccharide of traditional Chinese medicine and the flavonoid of traditional Chinese medicine are prepared according to the steps in Example 1, and the bursalin is prepared according to the steps in Example 2. Phosphate buffer solution consists of 6-10g sodium chloride, 0.05-0.5g potassium chloride, 1-1.2g disodium hydrogen phosphate, 0.05-0.5g potassium dihydrogen phosphate, 0.05-0.2g calcium chloride, containing 6 crystal water Dissolve 0.05-0.2g of magnesium chloride in 1000ml of double-distilled water and sterilize under high pressure at 116°C for 30 minutes.

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Abstract

The invention relates to a freeze-drying protective agent for a hog cholera live vaccine (spleen and lymph tissue origin), belonging to the technical field of veterinary biological products. Every 1000ml of phosphate buffer solution of the freeze-drying protective agent contains 30-100g of Chinese medicine polysaccharide, 20-60g of Chinese medicine flavone and 2-16mg of bursin, wherein the Chinese medicine polysaccharide and the Chinese medicine flavone are extracted from the following Chinese medicine compositions: sedum sarmentosum, radix tinosporae, Chinese fevervine, buffalo horn, mole cricket, elsholtzia, radix stephaniae tetrandrae, Chinese lobelia and beautiful sweetgum fruit. After an organism is immunized by the vaccine prepared from the freeze-drying protective agent, differentiation and proliferation of a B lymphocyte precursor of the organism can be facilitated, the speed of transcribing DNA (deoxyribonucleic acid) into mRNA (Messenger Ribonucleic Acid) in B lymphocyte is accelerated, the generation of proteins in the B lymphocyte is promoted, and thus the generation and antibody secretion capabilities of the B lymphocyte are enhanced, and the immunity of the organism can be improved.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to a freeze-dried protective agent for live vaccines derived from swine fever spleen and gonorrhea. Background technique [0002] Classical swine fever (CSF) is a Class A legal infectious disease of the International Organization for Animal Health (OIE) and China. It is one of the major animal diseases that are subject to compulsory immunization in my country, and is the "first killer" that endangers my country's pig industry. Swine fever is caused by swine fever virus and cannot be controlled with drugs. Vaccination is the most effective way. [0003] At present, the CSF vaccines on the market at home and abroad mainly include CSF splenic vaccine, CSF cell vaccine and CSF subunit vaccine. As a classical-refined live vaccine of classical swine fever and splenic gonorrhea in vaccines, it still has an irreplaceable role. [0004] The Chinese swine ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/46A61K47/36A61K47/18A61K39/39A61K39/187A61P31/14
Inventor 沈建军张秀文李阳
Owner 浙江美保龙生物技术有限公司
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