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Litchi seed dripping pill quality detection method

A quality detection method, the technology of nuclear dripping pills, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of poor specificity and achieve effective detection, reasonable step design, and good specificity

Inactive Publication Date: 2015-08-05
GUANGZHOU HOSPITAL OF TRADITIONAL CHINESE MEDICINE
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  • Abstract
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  • Claims
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Problems solved by technology

[0004] At present, the existing literature generally adopts the "iron salt catalyzed colorimetric method" for the determination of the content of proanthocyanidins in litchi core. The phenolic hydroxyl group, so this method is less specific

Method used

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Embodiment 1

[0014] The chromatographic column that the method of the present invention adopts is CAPCELL PAK C 18 UG120S3, the specification is: 4.6mm×100mm, 5μm; the mobile phase is acetonitrile-0.1% phosphoric acid (volume ratio: 10:90), the flow rate is 1.0mL / min, the column temperature is 30°C; the detection wavelength is 280nm. The number of theoretical plates is at least 2000 based on proanthocyanidins. Wherein, 0.1% phosphoric acid means that the aqueous solution concentration of phosphoric acid is 0.1% (volume percentage).

[0015] Follow the steps below to carry out quality inspection on litchi seed dripping pills:

[0016] 1. Accurately weigh the proanthocyanidin reference substance, place it in a volumetric flask, add methanol to dissolve, constant volume, shake well, and prepare a reference substance solution containing 1.15 mg proanthocyanidin per milliliter.

[0017] 2. Take lychee seed dropping pills (about 20), grind them, weigh 0.5g accurately, add 40 mL of acetone with...

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Abstract

The present invention provides a litchi seed dripping pill quality detection method, which comprises: taking a procyanidin reference substance, and adding methanol to dissolve so as to prepare a reference substance solution; taking litchi seed dripping pills, grinding, precisely weighing 0.5 g of the litchi seed dripping pills, adding to 40 mL of acetone with a volume concentration of 70% to carry out ultrasonic extraction, filtering, washing the residue with acetone, combining the filtrates, carrying out water bath heating so as to completely evaporate the acetone, dissolving the residue with methanol and transferring into a measuring flask, diluting with methanol to achieve the scale, shaking to achieve a uniform state, filtering with a 0.2 [mu]m micro-pore filtration membrane, and adopting the subsequent filtrate as a test solution; precisely sucking the reference substance solution to load, and measuring the peak area to obtain a standard curve equation; and precisely sucking the test solution to load, measuring the peak area, and substituting into the standard curve equation to calculate the procyanidin content. According to the present invention, the specificity of the method is good, and the procyanidin content in the litchi seed dripping pill can be well, efficiently, stably, quickly and sensitively determined so as to effectively detect the litchi seed dripping pill quality and ensure the medication safety.

Description

technical field [0001] The invention belongs to the field of medicine quality control, in particular to a method for detecting the quality of litchi kernel dripping pills, in particular to a method for measuring the content of proanthocyanidins in litchi kernel dripping pills. Background technique [0002] Proanthocyanidins is a general term for a large class of polyphenolic compounds that widely exist in plants. It is mainly composed of monomers, dimers, trimers and decamers of different amounts of catechin and epicatechin. Strong ability to oxidize and scavenge free radicals and improve human microcirculation. Modern pharmacological studies have shown that proanthocyanidins also have anti-inflammatory, anti-tumor, anti-radiation, anti-aging, improve immunity, improve bone formation, anti-diabetes, lower blood fat, lower blood pressure, promote wound healing and tissue repair activities and anti-cancer effects (Lexus Qun et al., Progress in Pharmacological Research of Proa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 郭洁文钟世顺黄志刚
Owner GUANGZHOU HOSPITAL OF TRADITIONAL CHINESE MEDICINE
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