Preparation method of imprinted polymers of core-shell type molecules, products of imprinted polymers of core-shell type molecules, and application of products
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A technology of molecular imprinting and polymers, applied in the preparation of sugar derivatives, chemical instruments and methods, sugar derivatives, etc., can solve the problems of difficult elution of target molecules, low adsorption capacity, slow mass transfer speed, etc., and the method is simple , the effect of high purity
Inactive Publication Date: 2015-07-22
EAST CHINA UNIV OF SCI & TECH
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Embodiment 1
[0026] Synthesis of Lincomycin A Molecularly Imprinted Polymer
[0027] Step 1: Synthesis of core layer polymer (seed)
[0028] Add NaHCO3 (0.238g); sodium dodecyl sulfate (SDS) (0.228g), distilled water 130mL into a 250mL Erlenmeyer flask, stir slowly, heat the water bath to 90°C, and then add the monomer mixture (MMA 9.27ml; EDMA (0.886ml), N2 to remove O2, initiator ammonium persulfate 0.12g, put into constant temperature water bath shaker, at 250 rpm, 80°C, continue to react for 24h.
[0029] Step 2: Synthesis of core-shell imprinted polymers
[0030] Add 18mL of water and SDS (0.10g) into a 250ml Erlenmeyer flask, then add the monomer mixture (EDMA5.43ml; AM 0.05; template molecule lincomycin 0.365g; seeds 29.04ml; Pore agent cyclohexanol 2ml and dodecanol 1ml), N2 remove O2, add initiator ammonium persulfate 0.10g, in warm water bath shaker, at 250 speed, 60 ℃ after continuous reaction for 24h, take out, ice water cooling , to abort the reaction.
Embodiment 2
[0032] Example of elution of template molecules, wash with acetone and methanol to remove self-polymerized and unreacted monomers; then put the polymer in a mixture of methanol and acetic acid (9:1 by volume) to remove target molecules. Wash with aqueous solution.
Embodiment 3
[0034] Equilibrium binding experiment at room temperature, with lincomycin A as the target, dissolve it in water, prepare 10 concentration points from 0.1-1.0mg / ml, the volume is 10ml, add 50mg polymer, shake at room temperature for 24h , the adsorption capacity of lincomycin A can reach 62.66mg / g.
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Abstract
The invention relates to a preparation method of imprinted polymers of core-shell type molecules, products of the imprinted polymers of the core-shell type molecules, and the application of the products. The preparation method comprises the following steps: synthesizing a seed emulsion by adopting a monomeric compound shown as a formula II and a monomeric compound shown as a formula III; using the seed emulsion as a core, adopting the monomeric compound shown as the formula I and the monomeric compound shown as the formula II to carry out random copolymerization with the seed emulsion so as to obtain the imprinted polymers of the core-shell type molecules. According to the preparation method of the imprinted polymers of the core-shell type molecules, the products of the imprinted polymers of the core-shell type molecules, and the application of the products, which are disclosed by the invention, can be used for extracting lincomycin A with higher purity from lincomycin and analogues of the lincomycin; under the water-phase condition, or in a water solution, the lincomycin A, the lincomycin B and other analogous ingredient components can be separated, so that the lincomycin A with higher purity is obtained; a better separation result can be obtained when the preparation method is combined with the columns chromatography.
Description
technical field [0001] The present invention relates to the field of drug separation, in particular to the separation of lincomycin components, specifically a core-shell molecule applied to lincomycin and its analogs to separate lincomycin A with high purity Preparation method of imprinted polymer, its product and application of the product. Background technique [0002] Lincomycin is an antibiotic obtained by Mason from the culture of Streptomyces in 1962. It has a strong antibacterial effect on Gram-positive bacteria. It mainly acts on the 50S subunit of the ribosome of sensitive bacteria and prevents peptide Chain extension, especially the antibacterial effect on streptococcus, staphylococcus aureus and anaerobic bacteria is particularly obvious, and has a significant effect in the treatment of many infectious diseases. Lincomycin is mainly used clinically for various infections caused by sensitive bacteria, such as pneumonia, meningitis, endocarditis, tonsillitis, erysi...
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