Method for determining living plant unidirectional ion absorption speed by liquid scintillation counting method and special sample pipe of method

Abstract

The invention discloses a special sample pipe for a method for determining a living plant unidirectional ion absorption speed by using a liquid scintillation counting method. The special sample pipe comprises a top cover, a pipe body and a base, wherein the top cover, the pipe body and the base are circular; the top cover is covered on the upper end of the pipe body; and the base is fixedly connected with the lower end of the pipe body. According to the method for determining the living plant unidirectional ion absorption speed by using the liquid scintillation counting method, the measurement of the living plant unidirectional ion absorption speed can be solved by the simple and efficient liquid scintillation counting method and the instant speed determination which cannot be finished by a common determination method can be effectively solved. The volume of the special sample pipe is smaller than that of a common sample pipe so that the use amount of a radioactive solution is reduced; a sealing gasket cushion is designed so that the safety of operators and the uniformity of uniformly shaking the sample are guaranteed; a flexible model can be put into a common sample bottle to be determined, and is made of a polypropylene material so that the special sample pipe is not easy to break and radioactive leakage is not caused; and the special sample pipe has a simple structure and is convenient to operate, and a testing result can accurately represent the actual condition of ion transportation.

Description

technical field [0001] The invention relates to the field of measuring radioactive ions, in particular to a liquid scintillation counting method for measuring the unidirectional ion absorption rate of living plants and a special sample tube thereof. Background technique [0002] The determination of the one-way ion absorption rate of living plants is of great significance for the study of the absorption and transport of plant minerals and nutrients. The determination of unidirectional ion uptake rate can be done by patch clamp technique and radioactive ion tracer technique. Patch clamp technology has high requirements for personnel due to its fine cell micromanipulation, and can only measure the ion flow rate at the cell level. In addition, it is difficult to obtain cell experimental materials, so the more common method is radioactive ion tracer. technology. At present, the radioactive ion tracer technology can introduce a small amount of radioactive tracer into the corre...

AI Technical Summary

Problems solved by technology

However, in the existing literature, different researchers have adopted different experimental processing steps, sampling methods, sample processing methods, counter types, etc. for different plant types, ray types, transpiration and other factors, resulting in different experimental results. The lack of comparability between them affects the reference and reference of later researchers

There is currently a lack of a concise and uniform method for measuring unidirectional ion uptake rates in living plants

At the same time, the volume of sample bottles used in liquid scintillation counters is generally about 10ml, and more test solutions need to be prepared; at the same time, sample bottles are special consumables for the machine, especially if the sample tubes of some imported liquid scintillation counters are consumed at one time. then the cost is too high

At present, there is a lack of a low-cost sample bottle replacement tube that can complete the determination with only a small amount of test solution.

Images