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Stable serum urea detection method with strong antijamming capability and reagents

A technology of detection reagents and detection methods, applied in the measurement of color/spectral characteristics, etc., can solve the problems of high false results and low urease, and achieve the effect of low price, improved stability, accuracy and stability

Inactive Publication Date: 2015-07-01
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this method is that it is susceptible to the influence of ammonium ions in the environment, resulting in falsely high results. In addition, the activity of urease is easily inhibited by fluoride, resulting in low results.

Method used

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  • Stable serum urea detection method with strong antijamming capability and reagents
  • Stable serum urea detection method with strong antijamming capability and reagents
  • Stable serum urea detection method with strong antijamming capability and reagents

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The urea detection kit described in this embodiment includes reagent R1 and reagent R2.

[0042] Reagent R1:

[0043] PIPES (piperazine-1,4-diethanesulfonic acid) buffer solution (pH=7.6) 100mmo1 / L

[0044] α-ketoglutaric acid 20mmo1 / L

[0045] Glutamate dehydrogenase 800U / L

[0046] ADP 2mmo1 / L

[0047] BSA 1g / L

[0048] Trehalose 2g / L

[0049] Sucrose 20g / L

[0050] APG (alkyl glycoside) 2g / L

[0051] Sodium azide (NaN3) 0.5g / L

[0052] Reagent R2:

[0053] PIPES (piperazine-1,4-diethanesulfonic acid) buffer solution (pH=9.4) 100mmo1 / L

[0054] Urease 10KU / L

[0055]NADH 1.2mmo1 / L

[0056] BSA 1g / L

[0057] Trehalose 2g / L

[0058] Sucrose 20g / L

[0059] APG (alkyl glycoside) 2g / L

[0060] Sodium azide (NaN3) 0.5g / L.

[0061] The usage method of this embodiment reagent:

[0062] The urea detection reagent described in this embodiment is determined by a fixed-time method using a fully automatic biochemical analyzer, such as Mindray BS-800 fully autom...

Embodiment 2

[0067] Interference test: Take fresh mixed serum, divide it into 2 equal parts, and then divide each equal part into 4 equal parts, add different interfering substances, so that the concentration in the serum reaches the requirements in Table 2. Then, the reagents obtained in Example 1 were used to compare and measure the content of UREA in the serum at the same time as the UREA reagents that are common and recognized in the market. Relative deviation (%) = (measuring mean value of interference samples - measuring mean value of control samples) / measured mean value of control samples × 100%.

[0068] It can be seen from Table 2 that the reagent of Example 1 has no obvious interference on the test results when ascorbic acid≤400mg / dL, bilirubin≤40mg / dL, and hemoglobin≤450 mg / dL. However, the reagents of the control group were significantly interfered in the presence of the above-mentioned concentration of interfering substances, which shows that the anti-interference performance ...

Embodiment 3

[0072] Correlation experiment: using the formula in Example 1 to prepare reagents, and conducting a control test with the urea kit of a company approved by the State Food and Drug Administration, which is common in the market, and testing 20 clinical serum samples at the same time, the test results are shown in Table 3 . And obtained the correlation curve of the two reagents (such as figure 1 Shown), the test results show that the correlation coefficient of the two kits is 0.9974, indicating that there is a great correlation between the two.

[0073] Table 3 Example 1 reagent and market common and approved urea assay kit comparative detection results

[0074] 。

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Abstract

The invention provides a stable serum urea detection method with a strong antijamming capability and reagents. The stable serum urea detection method with the strong antijamming capability adopts an enzyme coupling rate method, can effectively detect the content of serum urea, and has the advantages of strong antijamming capability, good stability and the like. The reagent comprises a reagent R1 and a reagent R2, and a new buffer system and a stabilizer are adopted, so that the stability of the reagents is remarkably improved. Meanwhile, a novel surfactant alkyl polyglucoside is adopted, so that not only is the measuring performance remarkably improved, but also the stability and the antijamming capability of the reagent are enhanced, and the stable serum urea detection method with the strong antijamming capability and the reagent can fully meet the clinical requirement.

Description

technical field [0001] The invention relates to a detection method and a reagent for clinical determination of urea content in serum, and belongs to the technical field of clinical in vitro detection. Background technique [0002] Urea is a nitrogenous end product of protein catabolism. Urea nitrogen accounts for about 75% of the non-protein nitrogen in the blood. It is synthesized by ammonia in the liver and is the product of protein deamination. Filtration of urea from the blood into urine by the glomeruli is the primary method of eliminating excess nitrogen from the body. Blood urea nitrogen testing is used in the diagnosis and treatment of certain kidney diseases and metabolic disorders. [0003] Blood urea levels are a measure of renal function as well as prerenal and postrenal states. Elevations of urea due to prerenal factors include cardiac decompensation, dehydration, or increased protein catabolism. Renal factors with increased levels are acute glomerulonephriti...

Claims

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Application Information

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IPC IPC(8): G01N21/31
Inventor 甘宜梧李志明谭柏清
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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