A kind of method of inducing sporulation of filamentous fungus
A technology of hyphomycetes and strains, applied in the field of microorganisms, which can solve the problems of less sporulation, insignificant effects, and differences in conidia characteristics, and achieve the effects of mild cultivation conditions, avoiding repeated cultivation, and reducing workload
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Embodiment 1
[0033] Take a circular filter paper, cut out 4 filter paper holes evenly on the filter paper, place it flat on a petri dish, add an appropriate amount of distilled water to soak the filter paper, and keep it sterilized at a temperature of 121°C and a pressure of 120kPa for 20 minutes. , remove the filter paper.
[0034] To prepare agar medium, sterilize the prepared agar medium at a temperature of 121°C and a pressure of 120kPa, then add it to the sterilized petri dish, and the amount of each petri dish is 16ml. After solidification, agar culture plates were made.
[0035] Spread the sterilized filter paper on the surface of the agar culture plate with sterile tweezers, so that there is no gap or bubble between the filter paper and the agar culture plate.
[0036] Then use a sterile needle to pick an appropriate amount of non-sporogenous hyphomycete fungi, and inoculate them on the modified agar culture plate at the center of each filter paper hole on the filter paper.
[00...
Embodiment 2
[0039] Take a circular filter paper with a diameter of 70mm, cut out 5 filter paper holes with a diameter of 15mm evenly on the filter paper, place it flat in a petri dish with a diameter of 90mm, add an appropriate amount of distilled water to soak the filter paper, and heat the filter paper at a temperature of 121 After maintaining the sterilization for 20 minutes under the condition of ℃ and pressure of 120kPa, take out the filter paper.
[0040] Add 15g of agar powder, 8g of glucose, 8g of starch, 4g of sodium carboxymethylcellulose, 8g of peptone, 3g of magnesium sulfate, 3g of potassium dihydrogen phosphate, 4g of ferrous sulfate, and 75mg of vitamin B in 1000ml of water 1 , stir evenly, and prepare agar medium, sterilize the prepared agar medium at a temperature of 121°C and a pressure of 120kPa, then add it to the sterilized petri dish, and the amount of each petri dish is 18ml. After the agar medium is solidified, make an agar culture plate.
[0041] Spread the steri...
Embodiment 3
[0046] Take a circular filter paper with a diameter of 70mm, cut out 6 filter paper holes with a diameter of 12mm evenly on the filter paper, place it flat in a petri dish with a diameter of 90mm, add an appropriate amount of distilled water to soak the filter paper, at a temperature of 121 After maintaining the sterilization for 20 minutes under the condition of ℃ and pressure of 120kPa, take out the filter paper.
[0047] Add 18g of agar powder, 6g of glucose, 6g of starch, 4g of sodium carboxymethylcellulose, 7g of peptone, 4g of magnesium sulfate, 4g of potassium dihydrogen phosphate, 3g of ferrous sulfate and 70mg of vitamin B in 1000ml of water 1 , stir evenly, and prepare agar medium, sterilize the prepared agar medium at a temperature of 121°C and a pressure of 120kPa, then add it to the sterilized petri dish, and the amount of each petri dish is 16ml. After the agar medium is solidified, make an agar culture plate.
[0048] Spread the sterilized filter paper on the s...
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