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A kind of method of inducing sporulation of filamentous fungus

A technology of hyphomycetes and strains, applied in the field of microorganisms, which can solve the problems of less sporulation, insignificant effects, and differences in conidia characteristics, and achieve the effects of mild cultivation conditions, avoiding repeated cultivation, and reducing workload

Active Publication Date: 2018-05-25
WEIFANG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, under the existing culture technology, in the process of isolation and cultivation of hyphosporium fungi, it is easy to produce conidia or difficult to produce conidia during artificial culture, which makes it impossible to carry out the identification research of hyphosporium fungi
At the same time, the observation of sporulation phenotype cannot be carried out at the same time as the production of conidia, and multiple re-cultivation is often required to observe the sporulation phenotype, which consumes a lot of time and experimental costs
[0004] At present, the main methods for promoting hyphosporium sporulation include colony scratching method, ultraviolet light irradiation method, freezing stimulation method, dark / light alternate culture method, etc., but these methods all have the following disadvantages: (1) the ability to promote sporulation The effect is not obvious, the amount of sporulation is small, and the sporulation phenotype is difficult to observe; (2) it is difficult to accurately grasp the treatment time, and improper treatment time will inhibit the sporulation or death of the hyphosporium fungus; (3) the culture cycle is long, often after a long period of time (4) The characteristics of conidia produced are unstable, and there are large differences in the characteristics of conidia produced under different culture conditions

Method used

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  • A kind of method of inducing sporulation of filamentous fungus
  • A kind of method of inducing sporulation of filamentous fungus
  • A kind of method of inducing sporulation of filamentous fungus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Take a circular filter paper, cut out 4 filter paper holes evenly on the filter paper, place it flat on a petri dish, add an appropriate amount of distilled water to soak the filter paper, and keep it sterilized at a temperature of 121°C and a pressure of 120kPa for 20 minutes. , remove the filter paper.

[0034] To prepare agar medium, sterilize the prepared agar medium at a temperature of 121°C and a pressure of 120kPa, then add it to the sterilized petri dish, and the amount of each petri dish is 16ml. After solidification, agar culture plates were made.

[0035] Spread the sterilized filter paper on the surface of the agar culture plate with sterile tweezers, so that there is no gap or bubble between the filter paper and the agar culture plate.

[0036] Then use a sterile needle to pick an appropriate amount of non-sporogenous hyphomycete fungi, and inoculate them on the modified agar culture plate at the center of each filter paper hole on the filter paper.

[00...

Embodiment 2

[0039] Take a circular filter paper with a diameter of 70mm, cut out 5 filter paper holes with a diameter of 15mm evenly on the filter paper, place it flat in a petri dish with a diameter of 90mm, add an appropriate amount of distilled water to soak the filter paper, and heat the filter paper at a temperature of 121 After maintaining the sterilization for 20 minutes under the condition of ℃ and pressure of 120kPa, take out the filter paper.

[0040] Add 15g of agar powder, 8g of glucose, 8g of starch, 4g of sodium carboxymethylcellulose, 8g of peptone, 3g of magnesium sulfate, 3g of potassium dihydrogen phosphate, 4g of ferrous sulfate, and 75mg of vitamin B in 1000ml of water 1 , stir evenly, and prepare agar medium, sterilize the prepared agar medium at a temperature of 121°C and a pressure of 120kPa, then add it to the sterilized petri dish, and the amount of each petri dish is 18ml. After the agar medium is solidified, make an agar culture plate.

[0041] Spread the steri...

Embodiment 3

[0046] Take a circular filter paper with a diameter of 70mm, cut out 6 filter paper holes with a diameter of 12mm evenly on the filter paper, place it flat in a petri dish with a diameter of 90mm, add an appropriate amount of distilled water to soak the filter paper, at a temperature of 121 After maintaining the sterilization for 20 minutes under the condition of ℃ and pressure of 120kPa, take out the filter paper.

[0047] Add 18g of agar powder, 6g of glucose, 6g of starch, 4g of sodium carboxymethylcellulose, 7g of peptone, 4g of magnesium sulfate, 4g of potassium dihydrogen phosphate, 3g of ferrous sulfate and 70mg of vitamin B in 1000ml of water 1 , stir evenly, and prepare agar medium, sterilize the prepared agar medium at a temperature of 121°C and a pressure of 120kPa, then add it to the sterilized petri dish, and the amount of each petri dish is 16ml. After the agar medium is solidified, make an agar culture plate.

[0048] Spread the sterilized filter paper on the s...

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Abstract

The invention discloses a method for inducing sporulation of hyphosporium fungus, which comprises the following steps: (1) take circular filter paper, evenly cut out more than 4 filter paper holes, lay it flat on a petri dish, add appropriate amount of distilled water to soak, Remove the filter paper after autoclaving. (2) Prepare and sterilize the agar medium, and then add it to the sterilized petri dish, the amount of each petri dish is 15-20ml, and make the agar culture plate after the agar medium is solidified. (3) Spread the sterilized filter paper on the surface of the agar culture plate. (4) Pick an appropriate amount of non-sporogenous hyphomycete fungi and inoculate them on the improved agar culture plate at the center of each filter paper hole. (5) Cover and seal the petri dish, and incubate at room temperature under natural light for 10 to 30 days. The method of the invention has simple operation, mild culture conditions, remarkable effect of inducing sporulation of hyphosporium fungi, is effective for most hyphosporium fungi, conidia are evenly distributed on filter paper, and is convenient for observing sporulation phenotypes.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a method for inducing sporulation of hyphosporium fungi. Background technique [0002] For a long time, the classification and identification of hyphomycetes have been mainly based on the morphological characteristics of the fungi, and the characteristics of conidia (shape, size, number of septa, surface decoration and appendages, etc.) are the main species-level classification criteria for hyphomycetes. The continuous appearance of conidia (sporulation phenotype) is also an important basis for the identification of hyphosporium species. [0003] However, under the existing culture technology, in the process of isolation and cultivation of hyphosporium fungi, it is easy to produce conidia or difficult to produce conidia during artificial culture, which makes it impossible to carry out the identification research of hyphosporium fungi smoothly. At the same time, the observ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N3/00
Inventor 潘好芹夏海波赵静李艳青
Owner WEIFANG UNIV OF SCI & TECH
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