Method of inducing hymphomycetes to spore

Abstract

The invention discloses a method of inducing hymphomycetes to spore. The method comprises the following steps: (1) circular filter paper is taken, more than four filter paper holes are formed by uniformly shearing the filter paper, the filter paper is flatly placed in culture dishes and is soaked by adding a proper amount of distilled water, and the filter paper is taken out after high temperature and high pressure sterilization; (2) an agar medium is prepared and is sterilized, then the agar medium is added into the sterilized culture dishes, the addition amount of the agar medium in each culture dish is 15-20ml, and agar culture plates are manufactured after the agar culture medium is solidified; (3) the sterilized filter paper is flatly paved on the surface of each agar culture plate; (4) a proper number of hymphomycetes strains which do not spore are selected and are inoculated on the improved agar culture plate at the center position of each filter paper hole; (5) culture dish covers cover and are sealed, and the culture dishes are cultured for 10-30 days at room temperature under natural light rays. The method is simple to operate, the culture conditions are mild, the effect of inducing the hymphomycetes to spore is obvious, the method is effective for most hymphomycetes, conidiums are uniformly distributed on the filter paper, and sporing phenotypes are convenient to observe.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a method for inducing sporulation of hyphosporium fungi. Background technique [0002] For a long time, the classification and identification of hyphomycetes have been mainly based on the morphological characteristics of the fungi, and the characteristics of conidia (shape, size, number of septa, surface decoration and appendages, etc.) are the main species-level classification criteria for hyphomycetes. The continuous appearance of conidia (sporulation phenotype) is also an important basis for the identification of hyphosporium species. [0003] However, under the existing culture technology, in the process of isolation and cultivation of hyphosporium fungi, it is easy to produce conidia or difficult to produce conidia during artificial culture, which makes it impossible to carry out the identification research of hyphosporium fungi smoothly. At the same time, the observ...

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Problems solved by technology

[0003] However, under the existing culture technology, in the process of isolation and cultivation of hyphosporium fungi, it is easy to produce conidia or difficult to produce conidia during artificial culture, which makes it impossible to carry out the identification research of hyphosporium fungi

At the same time, the observation of sporulation phenotype cannot be carried out at the same time as the production of conidia, and multiple re-cultivation is often required to observe the sporulation phenotype, which consumes a lot of time and experimental costs

[0004] At present, the main methods for promoting hyphosporium sporulation include colony scratching method, ultraviolet light irradiation method, freezing stimulation method, dark / light alternate culture method, etc., but these methods all have the following disadvantages: (1) the ability to promote sporulation The effect is not obvious, the amount of sporulation is small, and the sporulation phenotype is difficult to observe; (2) it is difficult to accurately grasp the treatment time, and improper treatment time will inhibit the sporulation or death of the hyphosporium fungus; (3) the culture cycle is long, often after a long period of time (4) The characteristics of conidia produced are unstable, and there are large differences in the characteristics of conidia produced under different culture conditions

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