A kind of Pseudomonas putida p.pao‑1 with phosphorus removal function and its domestication and cultivation method
A technology for domestication and cultivation of Pseudomonas putida, which is applied in the methods of using microorganisms, methods based on microorganisms, biochemical equipment and methods, etc., and can solve problems such as limiting the application of phosphorus accumulating bacteria and the effect of phosphorus removal strains on phosphorus removal ability.
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Embodiment 1
[0031] Pseudomonas putida strain P.PAO-1 forms a large amount of PHB primary accumulation in vivo and its ability to remove phosphorus under aerobic conditions:
[0032] Pseudomonas putida strain P.PAO-1 (preserved on August 26, 2013 in the General Microbiology Center of China Committee for Microorganism Culture Collection (CGMCC for short), the preservation number is CGMCC No.8073, the same below) was inoculated in 5ml sterilized enrichment medium (each L contains 3g beef extract, 10g peptone, 5g NaCl, 0.02g KH 2 PO 4 , Trace elements: each L contains 5g EDTA, 5gFeSO 4 ·7H 2 O, 1.6gCuSO 4 ·5H 2 O, 5g MnCl 2 4H 2 O, 50mg (NH 4 ) 6 Mo 7 o 24 4H 2 O, 50mgH 3 BO 3 , 10mgKI and 50mg CoCl 2 ·6H 2 (2, the same below) pre-cultured, and then inoculated into ten 100mL sterilized enrichment medium (250ml sterilized Erlenmeyer flask), respectively, at 30°C, 120r min -1 Under the conditions of shaking culture for 24 hours, washed with normal saline for 3 times, centrifuged...
Embodiment 2
[0038] Effect of adding Pseudomonas putida strain P.PAO-1 on the phosphorus removal ability of activated sludge after domestication culture
[0039] Under the same culture conditions, equal amounts of domesticated Pseudomonas putida strain P.PAO-1, activated sludge, and activated sludge + domesticated Pseudomonas putida strain P. PAO-1 (the addition ratio is 1:1) is used for anaerobic / aerobic alternate culture, in which anaerobic 5h, aerobic 3h, respectively take 5ml of bacterial liquid at the end of anaerobic culture and during aerobic culture, centrifuge, and measure the bacteria Phosphorus release, phosphorus uptake and COD in the centrifugate.
[0040] The activated sludge collected from the return sludge well of the sewage plant was used for the addition experiment of Pseudomonas putida strain P.PAO-1. Since the sewage plant adopts the anoxic / anaerobic / aerobic (A / A / O) activated sludge process, the sludge contains a large number of active phosphorus-accumulating bacteri...
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