Method for preparing platinum nanowires through helicoverpa armigera nuclear polyhedron extractives

A nuclear polyhedron and protein extract technology, applied in the field of nanomaterial preparation, can solve the problems of high equipment requirements, environmental hazards, harsh preparation conditions, etc., and achieve the effect of uniform shape, stable structure and simple operation

Active Publication Date: 2015-07-01
YANSHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The preparation conditions of these methods are harsh and require high equipment
And these methods often use organic solvents, which are harmful to the environment.

Method used

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  • Method for preparing platinum nanowires through helicoverpa armigera nuclear polyhedron extractives
  • Method for preparing platinum nanowires through helicoverpa armigera nuclear polyhedron extractives
  • Method for preparing platinum nanowires through helicoverpa armigera nuclear polyhedron extractives

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] After every 20 mg of polyhedrosis virus was purified, the precipitate was dissolved in 1 mL of ultrapure water to obtain a polyhedron suspension. Take 10mL polyhedron suspension and add 500μL alkaline hydrolysis solution, which is a mixed solution of 0.3mol / L sodium carbonate, 0.5mol / L sodium chloride and 0.03mol / L disodium ethylenediaminetetraacetic acid solution, let stand 30min. Then add 5 mL of chloroform and shake evenly, take the supernatant after centrifugation, adjust the pH value to 6 with NaOH solution, put the supernatant into an ultracentrifuge tube after standing in a refrigerator at 4 °C for 12 hours, discard the supernatant after ultracentrifugation, The precipitated spots were dissolved with ultrapure water to obtain polyhedrin extract, and the obtained extract was freeze-dried at minus 105 degrees.

[0018] Weigh the freeze-dried powder of polyhedrin protein extract to prepare 10 μg / mL extract solution, take 800 μL protein extract solution, add 200 μL ...

Embodiment 2

[0020] After every 20 mg of polyhedrosis virus was purified, the precipitate was dissolved in 1 mL of ultrapure water to obtain a polyhedron suspension. Take 10mL polyhedron suspension and add 200μL alkaline hydrolysis solution, which is a mixture of 0.3mol / L sodium carbonate, 0.5mol / L sodium chloride and 0.03mol / L disodium ethylenediaminetetraacetic acid solution, let stand 60min. Then add 4 mL of chloroform and shake evenly, take the supernatant after centrifugation, adjust the pH value to 7 with NaOH solution, put the supernatant into an ultracentrifuge tube after standing in a refrigerator at 4 °C for 24 hours, discard the supernatant after ultracentrifugation, The precipitated spots were dissolved with ultrapure water to obtain polyhedrin extract, and the obtained extract was freeze-dried at minus 105 degrees.

[0021] Weigh the freeze-dried polyhedron protein extract to prepare a 30 μg / mL extract solution, take 1 mL of the protein extract solution, add 200 μL of K solut...

Embodiment 3

[0024] After every 20 mg of polyhedrosis virus was purified, the precipitate was dissolved in 1 mL of ultrapure water to obtain a polyhedron suspension. Take 10mL polyhedron suspension and add 400μL alkaline hydrolysis solution, which is a mixed solution of 0.3mol / L sodium carbonate, 0.5mol / L sodium chloride and 0.03mol / L disodium ethylenediaminetetraacetic acid solution, let stand 90min. Then add 2.5mL chloroform and shake evenly, take the supernatant after centrifugation, adjust the pH value to 6.5 with NaOH solution, put the supernatant into an ultracentrifuge tube after standing in a refrigerator at 4°C for 18 hours, discard the supernatant after ultracentrifugation , using ultrapure water to dissolve the precipitated spots to obtain the polyhedrin protein extract, and freeze-dry the obtained extract at minus 105 degrees.

[0025] Weigh the freeze-dried powder of polyhedrin protein extract to prepare 40 μg / mL extract solution, take 1200 μL protein extract solution, add 20...

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Abstract

The invention relates to a method for preparing platinum nanowires through helicoverpa armigera nuclear polyhedron extractives. The method mainly includes the steps that after centrifugal purification is carried out on helicoverpa armigera nuclear polyhedrosis virus raw powder, resuspension with ultrapure water is carried out, alkaline hydrolysis liquid is added for processing, chloroform is added for even shocking, supernate is taken for overspeed centrifugation, ultrapure water is added for dissolving sediment spots, and then the polyhedrin extractives are obtained; a K2PtC16 solution is added into the obtained extractives, sufficient and even mixing is carried out, hatching is carried out in an air bath table at the indoor temperature for 20 h-50 h, a NaOH solution is used for adjusting the pH value to range from 7 to 9, and hatching is continuously carried out for 20 h to 50 h; a NaBH4 solution is added for reduction, and the platinum nanowires with net structures are obtained. The simple and environmentally-friendly platinum nanowire synthetic method which is even in structure and shape distribution, adjustable is size and better in stability is provided, and the prepared net-shaped platinum nanowires are even in shape and stable in structure and have the good potential application value on the aspects of fuel cell, biosensing, organocatalysis and the like.

Description

technical field [0001] The invention relates to a method for preparing nanometer materials, in particular to a method for preparing platinum nanowires. Background technique [0002] Noble metal nanomaterials exhibit unique properties such as volume effect, surface effect, electrocatalytic activity, and catalytic activity for various organic reactions. The effective control and study of the shape and size of noble metal nanocrystalline materials can greatly improve their catalytic activity, which can provide convenience for human production and life. Therefore, through high-energy ball milling method, sol-gel method, hydrothermal method, vapor deposition method, liquid deposition method, microemulsion method, template synthesis method, etc., people can obtain the structure, shape, size controllable, uniform distribution, and good stability. Noble metal nanomaterials. With the advancement and development of science and technology, people began to try to use the organic biomo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B22F9/24B82Y40/00C07K1/14
Inventor 高发明刘鹏庞桂桂罗京京刘辉孙慕雪
Owner YANSHAN UNIV
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