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A kind of extraction process of l-tryptophan

An extraction process and tryptophan technology, applied in the field of L-tryptophan extraction process, can solve problems such as low amino acid efficiency, and achieve the effects of improving fermentation yield, simple decolorization process, and saving industrial costs.

Active Publication Date: 2018-08-03
XINJIANG FUFENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The third aspect: low efficiency of amino acid production by strains

Method used

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  • A kind of extraction process of l-tryptophan
  • A kind of extraction process of l-tryptophan

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A kind of extraction process of L-tryptophan, it comprises the steps:

[0027] Step 1): Escherichia coli (Escherichia coli) ATCC 27325 (see Appl.Environ.Microbiol.October 1994 vol.60 no.10, 3724-3731) and Corynebacterium glutamicum (Corynebacterium glutamicum) ATCC 13032 ( Journal of Biotechnology , V104, September 2003, Pages5-25) were cultured to a concentration of 1×10 8 Each / mL of the bacterial liquid, mixed according to the volume ratio of 2:1 to obtain the mixed bacterial liquid, then inoculated into the fermentation medium (glucose 20g / L, yeast powder 12g / L, magnesium sulfate according to 2% (v / v) inoculum 2.5g / L, Ammonium Sulfate 2g / L, Citric Acid 2.5g / L, Dipotassium Hydrogen Phosphate 9g / L, Ferrous Sulfate 76mg / L, Manganese Sulfate 5mg / L, Sodium Sulfate 20mg / L, Zinc Sulfate 7mg / L , cobalt chloride 6mg / L, copper sulfate 0.9mg / L), temperature 37°C, dissolved oxygen control 20%, tank pressure 0.05MPa, pH 7.0, when the glucose in the fermentation medium is exhaust...

Embodiment 2

[0035] A kind of extraction process of L-tryptophan, it comprises the steps:

[0036] Step 1): Escherichia coli (Escherichia coli) CCTCC M 2011316 (see CN201110400855X) and Corynebacterium glutamicum (Corynebacterium glutamicum) ATCC13032 were cultivated to a concentration of 1×10 8 Each / mL of the bacterium solution is mixed according to the volume ratio of 3:1 to obtain the mixed bacterium solution, and then inoculated into the fermentation medium (glucose 20g / L, yeast powder 12g / L, magnesium sulfate according to 2% (v / v) inoculum 2.5g / L, Ammonium Sulfate 2g / L, Citric Acid 2.5g / L, Dipotassium Hydrogen Phosphate 9g / L, Ferrous Sulfate 76mg / L, Manganese Sulfate 5mg / L, Sodium Sulfate 20mg / L, Zinc Sulfate 7mg / L , cobalt chloride 6mg / L, copper sulfate 0.9mg / L), temperature 37°C, dissolved oxygen control 20%, tank pressure 0.05MPa, pH 7.0, when the glucose in the fermentation medium is exhausted, it will enter the production of sugar supplementation In the L-tryptophan stage, the c...

Embodiment 3

[0044] Feed breeding test prepared by the present invention:

[0045] Choose 100 piglets of 60 days, be divided into two groups, every group of 50, wherein the experimental group is raised with the feed prepared in the present embodiment 1, and the control group is fed with Tongwei Zhongda pig; Same and comparable. See Table 1 for the indicators detected after 10 weeks of feeding.

[0046] Table 1

[0047]

[0048]

[0049] Conclusion: the feeding effect of the feed prepared by the invention is better, the daily weight gain and feed-to-weight ratio are better than those commonly used in the market for pigs, and the cost is lower than that of the commercial feed, realizing the transformation of waste into wealth.

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Abstract

The invention belongs to the field of biotechnology, and discloses a process for extracting L-tryptophan, including: bacterial liquid fermentation, L-tryptophan micromembrane filtration, three-column series separation column separation, ultrafiltration decolorization, reverse osmosis , double-effect concentration, crystallization, drying, packaging and other processes to obtain high-quality L-tryptophan products. The present invention adopts three-column series decoupling column decoupling, ultrafiltration membrane decolorization and one-time crystallization to obtain L-tryptophan product, the process is simple and continuous production is possible, and process waste can be produced protein feed through comprehensive treatment, which is green and environment-friendly.

Description

technical field [0001] The invention belongs to the field of biotechnology, relates to the technical field of L-tryptophan production, in particular to an extraction process of L-tryptophan. Background technique [0002] The molecular formula for L-tryptophan is C 11 h 12 o 2 N 2 , with a molecular weight of 204.21 and a nitrogen content of 13.72%. L-Tryptophan is a neutral aromatic amino acid containing indole groups. It is silky luster, hexagonal flaky self-color crystal, odorless and sweet. The solubility in water is 1.14g / L (25℃) soluble in dilute acid Or dilute alkali, relatively stable in lye, decomposed in strong acid, slightly soluble in ethanol, insoluble in chloroform and ether. [0003] L-Tryptophan is one of the eight essential amino acids in human and animal life activities, and it plays an important role in the growth, development and metabolism of humans and animals. It is called the second essential amino acid, following methionine and lysine Amino acid...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P39/00C12P13/22C12R1/19C12R1/15
Inventor 郭英熙李学朋刘家宁杨瑞丽魏璐
Owner XINJIANG FUFENG BIOTECH
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