Aldosterone detection kit as well as preparation method and application thereof

An aldosterone and kit technology, applied in the field of detection kits, can solve the problems of radiation damage, narrow detection range, long time consumption, etc.

Inactive Publication Date: 2015-05-20
SHENZHEN NEW INDS BIOMEDICAL ENG
View PDF4 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, the above-mentioned radioimmunoassay has the defects of radioactive pollution, short half-life of markers, radiation damage to the operator, cumbersome operation, long time-consuming, low sensitivity, narrow detection range, and cannot be fully automated.
The traditional radioimmunoassay or enzyme-linked immunoassay method takes a long time to detect, and at the same time mainly relies on a series of cumbersome operations such as pure manual sample addition, which is inefficient and easily leads to large errors in experimental results; due to the incomplete enzymatic reaction and is susceptible to external interference factors Influence, such as temperature, time and material concentration, so the specificity of detection is low, the sensitivity is poor, and the detection range is narrow

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Aldosterone detection kit as well as preparation method and application thereof
  • Aldosterone detection kit as well as preparation method and application thereof
  • Aldosterone detection kit as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] (1) Labeling of ALD antigen, the specific steps are as follows:

[0060] Preparation of dialysate (F solution): Add Na to a 5000ml container 2 CO 3 14.31g and NaHCO 3 26.46g, add purified water to dilute to 4500ml, and place the prepared F solution on a magnetic stirrer for later use.

[0061] Choose a dialysis bag with a suitable cutoff (commonly used molecular weight of 14000), measure the size enough to accommodate solution F, tie one end tightly after wetting, and test for leaks with purified water 3 times (no leakage is required).

[0062] Take 100 μg of ALD antigen and adjust to 1 ml with 0.1 mol / L pH9.5 carbonic acid buffer (F solution). Put it into the dialysate, stir and dialyze at room temperature for 2 hours, add 300 μg of ABEI activated ester to the dialyzed solution, and react at 37°C for 2 hours.

[0063] The ligation product of ALD antigen and ABEI was purified by G-25 gel column.

[0064] D. 2 Solution preparation: Add 200ml of 0.5M phosphate buffe...

Embodiment 2

[0081] (1) Labeling of ALD antigen, the specific steps are as follows:

[0082] Preparation of dialysate (F solution): Add Na to a 5000ml container 2 CO 3 14.31g, NaHCO 3 26.46g, add purified water to dilute to 4500ml. The prepared F solution was placed on a magnetic stirrer for later use.

[0083] Choose a dialysis bag with a suitable cutoff (commonly used molecular weight of 14000), measure the size enough to accommodate solution F, tie one end tightly after wetting, and test for leaks with purified water 3 times (no leakage is required).

[0084] Take 100 μg of ALD antigen and adjust to 1 ml with 0.1 mol / L pH9.5 carbonic acid buffer (F solution). Put it into the dialysate, stir and dialyze at room temperature for 2 hours, add 300 μg of ABEI activated ester to the dialyzed solution, and react at 37°C for 2 hours.

[0085] The ligation product of ALD antigen and ABEI was purified by G-25 gel column.

[0086] D. 2 Solution preparation: add 200ml 0.5M phosphate buffer sa...

Embodiment 3

[0108] (1) labeling of anti-ALD antibody, the specific steps are as follows:

[0109] Preparation of dialysate (F solution): Add Na to a 5000ml beaker 2 CO 3 14.31g, NaHCO 3 26.46g, add purified water to dilute to 4500ml, and place the prepared F solution on a magnetic stirrer for later use.

[0110] Select a dialysis bag with a suitable cutoff (commonly used molecular weight of 14000), measure the size enough to accommodate solution F, tie one end tightly after wetting, and test for leaks with purified water 3 times (no leakage is required).

[0111] Take 1 mg of anti-ALD antibody and adjust it to 1 ml with 0.1 mol / L carbonic acid buffer (F solution) at pH 9.5. Put it into the dialysate, stir and dialyze at room temperature for 2 hours, add 300 μg of ABEI activated ester to the dialyzed solution, and react at 37°C for 2 hours.

[0112] The ligation product of anti-ALD antibody and ABEI was purified by G-25 gel column.

[0113] Prepare D by the method in embodiment 1 2 s...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
diameteraaaaaaaaaa
Login to view more

Abstract

The invention relates to a chemiluminescence immune detection kit for detecting aldosterone and a preparation method of the chemiluminescence immune detection kit. The kit comprises an aldosterone antigen and an anti-aldosterone antibody, wherein one of the aldosterone antigen and the anti-aldosterone antibody is marked with a tracing marker, and the other one is coated with magnetic spheres. The invention further relates to a method for detecting aldosterone concentration by using the kit. According to the chemiluminescence immune detection kit and the method disclosed by the invention, high detection sensitivity and accuracy can be achieved by measuring the concentration of aldosterone by using the kit disclosed by the invention.

Description

technical field [0001] The invention relates to a detection kit, in particular to a chemiluminescent immunoassay kit for detecting aldosterone. The invention also relates to a preparation method of the kit and a method of using the kit to detect aldosterone concentration. Background technique [0002] Aldosterone (ALD) is a mineralocorticoid synthesized and secreted by the zona glomerular cells of the adrenal cortex, and is normally regulated by the renin-angiotensin system. The renin-angiotensin-aldosterone system (renin-angiotensin-aldosterone system, RAAS) is a hormone system. When massive blood loss or blood pressure drops, the kidneys secrete renin, which catalyzes the hydrolysis of angiotensinogen to produce angiotensin Ⅰ (AI), AI basically has no biological activity, but is cleaved by angiotensin converting enzyme (Angiotensin Converting Emzyme, ACE) to form AII by cutting two amino acid residues at the C-terminus. AII has a high-efficiency vasoconstriction effect, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/74G01N33/533G01N21/64
CPCG01N33/74G01N33/533G01N33/54326G01N33/54333
Inventor 饶微李钦徐红彭露李武李婷华袁锦云
Owner SHENZHEN NEW INDS BIOMEDICAL ENG
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap