A preparation method of fluorescent nanocomposite with drug release function

A technology of fluorescent nano-composites, applied in the field of preparation of fluorescent nano-composites, can solve the problems of cumbersome monitoring methods and low sensitivity, and achieve the effect of expanding practicability

Inactive Publication Date: 2017-02-22
UNIV OF SCI & TECH BEIJING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a functional fluorescent nanocomposite that can realize sensitive, convenient and effective monitoring of the drug release site and release process in view of the shortcomings of existing drug release carriers, such as cumbersome and low sensitivity monitoring means for the drug release process. preparation method

Method used

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  • A preparation method of fluorescent nanocomposite with drug release function

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) The silver ammonia solution that the silver ion concentration is the fresh configuration of 1 millimole per liter is quickly added in the tannic acid solution that the concentration of equal volume is 0.1 millimole per liter, stirs rapidly at room temperature for half an hour to one hour, prepares Spherical silver nanoparticles with an average diameter of 25 nm. After the reaction is completed, the silver nanoparticles are centrifugally washed and dispersed in deionized water of equal volume for use;

[0023] (2) With a volume ratio of 1:5, add a freshly configured cystine solution with a concentration of 1 mmol per liter to a silver nanoparticle solution with a concentration of 10 nanomol per liter, stir at room temperature for 5 hours, and then centrifuge and wash Silver nanoparticles, the solution volume is concentrated 5 times;

[0024] (3) With a volume ratio of 250:1, in a concentration of 0.5 mg per milliliter of polylysine solution, a concentration of 5 mmo...

Embodiment 2

[0028] (1) Get the chloroauric acid solution that the mass fraction is 0.01%, boil, with the volume ratio 133:1, add the sodium citrate solution that mass fraction is 1% wherein, keep boiling for 30 minutes, the obtained average diameter is 25 nanometers of gold nanoparticles;

[0029] (2) Adjust the pH value of the solution to pH=8, and add a freshly configured cysteine ​​solution with a concentration of 1 mmol per liter to the gold nanoparticle solution prepared in step (1) at a volume ratio of 1:20 , stirring at room temperature for 5 hours, then centrifuging and washing the gold nanoparticles to remove unreacted cysteine, collecting the centrifuged gold nanoparticles and concentrating them by 5 times in volume;

[0030] (3) With a volume ratio of 250:1, a concentration of 1 mg per milliliter of polylysine solution was added to a concentration of adriamycin solution of 5 mmol per liter, and after stirring evenly, the pH value of the solution was adjusted to pH = 10, and th...

Embodiment 3

[0034] (1) Get the chloroauric acid solution that the mass fraction is 0.01%, boil, with the volume ratio 133:1, add the sodium citrate solution that mass fraction is 1% wherein, keep boiling for 30 minutes, the obtained average diameter is 25 nanometers of gold nanoparticles;

[0035] (2) Adjust the pH value of the solution to pH=8, and add the freshly configured 2-mercaptoethylamine solution with a concentration of 1 mmol per liter to the gold nanoparticle solution prepared in step (1) at a volume ratio of 1:20 , stirring at room temperature for 5 hours, then centrifuging and washing the gold nanoparticles to remove unreacted cysteine, collecting the centrifuged gold nanoparticles and concentrating them by 5 times in volume;

[0036] (3) With a volume ratio of 250:1, add a doxorubicin solution with a concentration of 5 mmol per liter to a polylysine solution with a concentration of 2 mg per milliliter, stir evenly, and adjust the pH value of the solution to pH =10, then add...

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Abstract

The invention belongs to the field of preparation of a functional nano-composite material and relates to a preparation method of a fluorescent nano-composite having drug-release function. The fluorescent nano-composite can be used for monitoring the release progress of a drug via changes of fluorescence intensity. A main body of the fluorescent nano-composite is a nano-composite which is of a core-shell structure formed by metal nanometer particles and biological macro-molecules; a biocompatible polymer (such as a polylysine molecule) and a medicinal adriamycin amycin molecule are cross-linked and polymerized on the surface of a metal nanometer particle to form a polymer shell layer for covering the adriamycin amycin molecule. When glutathione with a certain concentration exists in the environment, a disulfide bond in a crosslinking agent is cut off, and then adriamycin amycin is released from the polymer shell layer. Meanwhile, due to changes of the thickness of the shell layer, the release progress of the drug can be sensitively and effectively represented by detecting the changes of the fluorescence intensity of fluorescent molecules coating the surface of the nano-composite according to distance dependency of metal reinforced fluorescence effect. The functional nano-composite material disclosed by the invention has quite good application prospect in the field of sentinel release treatment of drugs.

Description

technical field [0001] The invention relates to the field of preparation of functional nanocomposite materials, in particular to a method for preparing a fluorescent nanocomposite with drug release function based on metal-enhanced fluorescence, which can characterize the release process of the drug loaded on the metal surface through the change of fluorescence intensity. Background technique [0002] At present, traditional chemotherapeutic drugs are facing many problems that need to be solved urgently, including the disadvantages of rapid clearance in the blood circulation, poor biodispersity, and high toxicity to non-cancerous cells. Compared with free drug molecules, nanoparticle-based drug carriers can effectively improve the pharmacological properties of drugs. Its main advantages are as follows: First, with the help of the high permeability and retention effect (Enhanced permeability and retention, EPR) of tumor cells, nanoparticles with a diameter of less than 200 nan...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/00A61K47/61A61K47/59A61K31/704A61P35/00
Inventor 李立东唐馥王淳王晓瑜
Owner UNIV OF SCI & TECH BEIJING
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