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Citrullus lanatus.convar megulaspemus (L.) skin extract and medical application and preparation method of composition

A technology of extract and melon rind, which is applied in the direction of medical preparations containing active ingredients, drug combinations, and pharmaceutical formulas, to achieve the effects of improving cardiovascular and cerebrovascular blood supply, delaying aging, and scavenging free radicals

Active Publication Date: 2015-05-20
SHIHEZI UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] It can be seen from the references that the research on the rind of the melon is limited to the analysis of the pectin components of the rind of the melon, and there are few reports on the extraction and evaluation of the biologically active substances of the rind of the melon and the development of the product.

Method used

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  • Citrullus lanatus.convar megulaspemus (L.) skin extract and medical application and preparation method of composition
  • Citrullus lanatus.convar megulaspemus (L.) skin extract and medical application and preparation method of composition
  • Citrullus lanatus.convar megulaspemus (L.) skin extract and medical application and preparation method of composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0229] Example 1: Extraction and preparation of guapi extract

[0230] Crush the rind of the melon, add the rind of the melon at a ratio of 1:8 (g / mL) to a certain volume fraction of 70% ethanol solution, soak for 24 hours, then decoct, and wait for 3 hours after boiling. After the solvent of the rind of the melon rind is cooled at an interval of 15 minutes, filter it with a sieve. Then change to 70% ethanol according to 1:6 (g / ml) and soak again for 24 hours, and decoct for 2 hours after boiling. After the solvent of the rind of the melon rind is cooled at an interval of 15 minutes, filter it with a sieve. Change to 70% ethanol and soak again at 1:4 (g / ml), and decoct for 2 hours after boiling. After the solvent of the rind of the melon rind is cooled at an interval of 15 minutes, filter it with a sieve. The rind extracts soaked three times were combined and suction filtered, the filtrate was concentrated under reduced pressure, and the concentrated solution was evaporated...

Embodiment 2

[0232] Embodiment 2: the purification of the rind extract

[0233] 30g of the n-butanol extract of the pumpkin rind extract prepared in Example 1 was subjected to macroporous resin adsorption operation. The n-butanol extract was completely dissolved and passed through the column. The water washing liquid has no Molish reaction, then add 2% NaHCO 3 Solution 100ml elution, discard NaHCO 3 The eluent is eluted with 70% ethanol by volume fraction. The eluent was concentrated and evaporated to dryness to obtain 8 g. Take 1500g of the pretreated macroporous adsorption resin, fill it in the corresponding chromatographic column, and carry out the macroporous resin adsorption operation on the 86g of the rind of pumpkin. The rind extract was completely dissolved and passed through the column. The water washing liquid has no Molish reaction, then add 2% NaHCO 3 Solution 100ml elution, discard NaHCO 3 The eluent is eluted with 70% ethanol by volume fraction. The eluate was concent...

Embodiment 3

[0234] Embodiment 3: Research on the chemical constituents of the extract of guapi

[0235] 1. Extraction and separation

[0236] The extract of guapi was subjected to silica gel column chromatography, petroleum ether-acetone gradient elution, and similar eluted fractions were combined by TLC to obtain 13 fractions DG1-DG13.

[0237] DG1: No obvious principal point

[0238] DG2: No obvious principal point

[0239] DG3 (68mg): After gel column chromatography, eluted with methanol, a total of 6 components of DG3a-f were obtained, of which component DG3d was subjected to repeated silica gel column chromatography, eluted with chloroform:methanol 10:1, and then gel Column purification yielded compound 1 (11.0 mg).

[0240] DG4 (90mg): After gel column chromatography and methanol elution, a total of 8 components of DG4a-h were obtained. Among them, component DG4g was obtained by repeated silica gel column chromatography and elution of chloroform: methanol: water 10:1:0.3 Compoun...

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Abstract

The invention relates to a citrullus lanatus.convar megulaspemus (L.) skin extract with medicinal application from citrullus lanatus.convar megulaspemus (L.) skin and a pharmaceutical composition containing the citrullus lanatus.convar megulaspemus (L.) skin extract as well as preparation, medical use and an analytical method thereof. The preparation method of the citrullus lanatus.convar megulaspemus (L.) skin extract comprises the following steps: crushing citrullus lanatus.convar megulaspemus (L.) skin; adding the citrullus lanatus.convar megulaspemus (L.) skin into a 50-90% ethanol solution in a ratio of 1:(4-10); soaking for 10-30 hours and decocting for 1-5 hours; cooling; filtering to obtain an extraction liquid; repeating the extraction step for 2-3 times; combining and filtering the extraction liquid; and concentrating and drying the filtrate to obtain the extract.

Description

Technical field: [0001] The present invention relates to a melon rind extract prepared from the husk of a melon (Citrullus lanatus. convar megulaspemus (L.)) with medicinal uses and a pharmaceutical composition containing the rind extract, as well as their preparation, medical use and Analytical method. Background technique: [0002] Melon Citrullus lanatus.convar megulaspemus L., also known as seed melon. It belongs to Cucurbitaceae and is a cultivated variety of common watermelon species. It is mainly distributed in Xinjiang, Inner Mongolia and Gansu, among which the quality produced in Gansu is the best. The annual output of melons in the country is millions of tons, but they are all used for seed extraction. However, the flesh and skin, which account for nearly 93% of the total mass, are discarded, which not only causes waste of resources, but also pollutes the farmland environment. According to historical records, "Punch melon into the heart, spleen and stomach, and t...

Claims

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Application Information

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IPC IPC(8): A61K36/42A23L1/29A61P39/06A61P31/04A61P29/00A61P37/04A61P39/00A61P9/10A61P17/00A61P3/06A61P7/00A23L33/00
Inventor 田丽萍王金辉薛琳张义晖湛圆圆李欠南张彬李守明
Owner SHIHEZI UNIVERSITY
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