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A biological control method for disease resistance and growth promotion of Clover

A technology of cloverleaf and biological fertilizer, applied in the fields of botanical equipment and methods, biocides, biological organic part treatment, etc., can solve the problems of poor resistance, low stability, and low stress resistance of spore-free bacteria , to achieve the effect of simple processing, good economic benefits, and easy industrialized large-scale production

Inactive Publication Date: 2016-09-14
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, non-spore bacteria have low stress resistance and poor resistance to dry high temperature, so the stability is not high when made into liquid or adsorbent

Method used

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  • A biological control method for disease resistance and growth promotion of Clover
  • A biological control method for disease resistance and growth promotion of Clover
  • A biological control method for disease resistance and growth promotion of Clover

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1) High-density culture of Bacillus subtilis (CICC 10339) producing beta glucanase, the starting density of Bacillus subtilis (CICC 10339) is 1.06×10 6 each / mL, inoculated into liquid medium (glucose 14g / L, beef extract 25g / L, potassium dihydrogen phosphate 2.45g / L, peanut cake powder 10g / L, pH=7.0, sterilized at 121°C for 20min), inoculated 2%, shake culture at 37°C for 24 hours, and the density of the bacterial solution is 2.25×10 9 cfu / mL to obtain a high-density bacterial suspension.

[0027] 2) Cut a total of 100kg of healthy branches of Clover into small sections for natural composting, spray 2L of the high-density bacterial suspension prepared in 1) when the internal temperature of the compost reaches 32°C, and continue composting until the internal temperature of the compost exceeds 43°C When the composting is over, it is stirred and cooled, and post-fermented in a natural state to become a bio-fertilizer rich in beta-glucanase-producing Bacillus subtilis, whic...

Embodiment 2

[0037] 1) High-density culture of Bacillus subtilis (CICC 10339) producing beta glucanase, the starting density of Bacillus subtilis (CICC 10339) is 1.88×10 6 each / mL, inoculated into liquid medium (glucose 14g / L, beef extract 25g / L, potassium dihydrogen phosphate 2.45g / L, peanut cake powder 10g / L, pH=7.0, sterilized at 121°C for 20min), inoculated Amount of 3%, shaking culture at 37°C for 24 hours, the density of the bacterial solution was 3.56×10 9 cfu / mL to obtain a high-density bacterial suspension.

[0038] 2) Cut a total of 100kg of healthy branches of Clover into small sections for natural composting, spray 3L of the high-density bacterial suspension prepared in 1) when the internal temperature of the compost reaches 32°C, and continue composting until the internal temperature of the compost exceeds 43°C When the composting is over, it is stirred and cooled, and post-fermented in a natural state to become a bio-fertilizer rich in beta-glucanase-producing Bacillus subti...

Embodiment 3

[0044] 1) High-density culture of Bacillus subtilis (CICC 10339) producing beta glucanase, the starting density of Bacillus subtilis (CICC 10339) is 1.76×10 6 each / mL, inoculated into liquid medium (glucose 14g / L, beef extract 25g / L, potassium dihydrogen phosphate 2.45g / L, peanut cake powder 10g / L, pH=7.0, sterilized at 121°C for 20min), inoculated The amount is 3%, cultured with shaking at 37°C for 24 hours, and the density of the bacterial solution is 2.25×10 9 cfu / mL to obtain a high-density bacterial suspension.

[0045] 2) Cut a total of 100kg of healthy branches of Clover into small sections for natural composting, spray 3L of the high-density bacterial suspension prepared in 1) when the internal temperature of the compost reaches 32°C, and continue composting until the internal temperature of the compost exceeds 43°C When the composting is over, it is stirred and cooled, and post-fermented in a natural state to become a bio-fertilizer rich in beta-glucanase-producing B...

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Abstract

The invention discloses a biological control method for achieving disease resistance and growth promotion for radix tetrastigme. The method includes the steps of making high-density bacterium suspension with a density of 109 cfu / mL to 1010 cfu / mL through bacillus subtilis, shearing healthy branches of radix tetrastigme into small segments for composting, spraying the high-density bacterium suspension of bacillus subtilis in the composting process, obtaining biological fertilizer capable of effectively restraining and killing radix tetrastigme pathological fungi after fermentation, and applying the biological fertilizer for achieving disease resistance and growth promotion for radix tetrastigme. In the method, the bacillus subtilis capable of producing beta glucanase is plant probiotics with effects on achieving disease resistance and growth promotion for radix tetrastigme, by adding the bacillus subtilis to the compost, the biological control effect on the radix tetrastigme pathological fungi is remarkably enhanced, and the yield of radix tetrastigme is improved. In the composting process, treatment is easy and convenient, no waste water is discharged, investment is small, the energy consumption is low, operation is easy, the industrial large-scale production is easy, and good economic benefits and wide application prospects are achieved.

Description

technical field [0001] The invention relates to the field of preparation of biological control fertilizers, in particular to a biological control method for anti-disease and growth-promoting of trifolium clover. Background technique [0002] Clover vine (scientific name: Tetrastigma hemsleyanum Diels et Gilg), also known as golden thread hanging gourd, snake monkshood, stone mouse, belongs to Rhamnaceae, herbaceous vine of grape family. The whole plant can be used as medicine, and the medicinal effect of underground tubers and fruits is the best. Clover blooms every year from May to autumn without fruiting. It has a long flowering period. The fruit is the size of mung beans, bright red in color, sweet in taste, cool in nature, and has nourishing effects. It is the best. Distributed in Zhejiang, Jiangxi, Fujian, Hubei, Hunan, Guangdong, Guangxi, Sichuan and other regions. [0003] The main disease that harms clover is leaf spot, and Alternaria alternata is one of the main p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G13/00A01N63/02A01P3/00C05F17/00
CPCA01G13/00A01N63/10C05F11/08C05F17/00Y02W30/40
Inventor 吴渊胡慧雯徐薇薇阮晖梁莹许丽珍吴孔华莫建平史金昕波缪丹俞雨吴盛彬徐腾洋
Owner ZHEJIANG UNIV
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