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Food and tableware coliform bacteria rapid detection scrip preparation and application

A technology of coliform bacteria and paper chips, which is applied in the direction of biochemical equipment and methods, and microbial measurement/inspection, etc., can solve problems such as the inability to use tableware for coliform bacteria detection, difficult colony counting, and unclear colony formation, etc., to achieve Low cost, accurate results, effect of reducing interference effects

Inactive Publication Date: 2015-05-13
HUBEI UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many researches on rapid detection of coliforms at home and abroad, such as rapid detection of coliforms in food with Chinese invention patent (200710013010.9), rapid detection of wet coliforms (200420059068.9), large intestine in liquid food Bacteria rapid test paper (200610069663.4), the above patents can be used for the detection of coliform bacteria, but these patent test papers do not have cold water gel components, so the formation of colonies is not clear, and colony counting is difficult
A Chinese invention patent for a test piece for rapid detection of microorganisms and its preparation method and application (200910007917.3). This patent adds cold water gel components, but this patent can only be used for the detection of coliform bacteria in food, not for catering Detection of coliform bacteria

Method used

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  • Food and tableware coliform bacteria rapid detection scrip preparation and application
  • Food and tableware coliform bacteria rapid detection scrip preparation and application
  • Food and tableware coliform bacteria rapid detection scrip preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: the preparation method of rapid detection test strip

[0029] (1) Preparation of carrier paper sheets: cut the chromatographic filter paper into 5cm×5cm size, irradiate or autoclave (after high pressure, dry at constant temperature and vacuum at 50°C) for later use.

[0030] (2) Preparation of the inner packaging bag: Press the high-temperature-resistant polypropylene plastic film bag into a joint bag with a size of 5.1cm×5.1cm according to the test requirements.

[0031] (3) Basic medium configuration (1L): tryptone 10.0-15.0g; beef extract 3.0-5.0g; sodium chloride 5.0-6.0g dipotassium hydrogen phosphate (K 2 HPO 4 2H 2 O) 4.0-5.0 g; lactose 15.0-20.0 g; bromocresol purple content 60.0-70.0 mg; deoxycholic acid content 1.0-1.5 g; 20.0g / L red tetrazolium (TTC) solution 5-7 mL.

[0032] (4) Cold water gel configuration (mass percentage): 0.2-0.4% sodium polyacrylate, 0.1-0.3% xanthan gum, 0.1-0.2% carrageenan and 0.1-0.2% konjac gum.

[0033] (5) ...

Embodiment 2

[0036] Embodiment 2: the specificity test of rapid detection test strip

[0037] Cultivate Bacillus subtilis, Staphylococcus aureus, Salmonella, Vibrio parahaemolyticus, and Listeria monocytogenes overnight in shake flasks, centrifuge 1 mL of the bacterial suspension at 8000 r / min for 5 min, resuspend the bacterial cells in 1 mL of sterile water, According to the ten-fold dilution, dilute to a certain multiple (10 3 -10 4 ), drop 1mL of the bacterial suspension onto the test paper, incubate at 36°C±1°C for 16-24h, observe the growth of the colony on the paper, and study the specificity of the test paper. 5 parallel groups were set up, and the experiment was repeated 3 times.

[0038]The test results show that only when Escherichia coli exists, the test strip will turn yellow and show red spot colonies or flaky blush on the yellow background; while Bacillus subtilis, Staphylococcus aureus, Salmonella, Vibrio parahaemolyticus, When Listeria monocytogenes is present, the ...

Embodiment 3

[0039] Embodiment 3: the interfering test of rapid detection test strip

[0040] At the same time, Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Salmonella, Vibrio parahaemolyticus, and Listeria monocytogenes were cultured overnight in shake flasks, and 0.5mL of the bacterial suspension was centrifuged at 8000r / min for 5min. to a certain multiple (10 3 -10 4 ), followed by mixing 0.5ml of Bacillus subtilis, Staphylococcus aureus, Salmonella, Vibrio parahaemolyticus, and Listeria monocytogenes with 0.5ml of Escherichia coli suspension, resuspended in 1mL of sterile water, Add 1mL of bacterial suspension dropwise to the detection paper, incubate at 36°C±1°C for 16-24 hours, observe the growth of colonies on the paper, and study whether other bacteria interfere with the growth of E. coli. 5 parallel groups were set up, and the experiment was repeated 3 times.

[0041] The test results show that as long as E. coli exists in the mixed bacterial solution, the t...

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Abstract

The invention provides a coliform bacteria rapid detection cold water gel scrip, and the detection cold water gel scrip is a hygiene inspection product special for rapid detection of coliform bacteria number in food and tableware. Through reasonable ratio of culture medium components and cold water gel components, scientific production process and the appropriate packaging, the product cost is low, the operation method is simple and easy to operate, detection procedure is simple and fast, so that cumbersome tube fermentation culture and physical and chemical test are simplified and can be completed in one step, sensitivity is good, specificity is strong, results are accurate, the national standard method coincidence rate is high; meanwhile antibacterial substances are added into the culture medium to reduce the interference of other microorganisms. The rapid detection scrip can achieve quick and accurate detection and qualitative and quantitative analysis of coliform bacteria, and can be applied to the rapid detection of the coliform bacteria in food and tableware.

Description

technical field [0001] The invention relates to a paper sheet for rapid detection of coliforms in food and tableware, which is a qualitative and quantitative detection test paper specially used for rapid detection of coliforms in food and tableware. Background technique [0002] Coliform bacteria are widely distributed and widely exist in feces of warm-blooded animals and in nature. Investigations and studies have shown that coliform bacteria mostly exist in the feces of warm-blooded animals, places where humans frequently move, and places with fecal contamination. The pollution of human and animal feces to the external environment is the main reason for the existence of coliform bacteria in nature. Most of the feces are dominated by typical Escherichia coli, while other types of coliforms are more common in the external environment. Coliform bacteria is one of the important indicators to evaluate food hygiene quality, and it has been widely used in food hygiene work at ho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/10C12Q1/06
Inventor 张毅代俊周梦舟沈黎贾振华张超李冬生汪超徐宁胡勇
Owner HUBEI UNIV OF TECH
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