Mushroom diet food and preparation method thereof
A technology for diet food and shiitake mushrooms, which is applied in food preparation, food science, application and other directions, can solve the problems of volatile inactivation, unfavorable production of industrialized products, etc. active effect
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Embodiment 1
[0016] CaCl 2 - Preparation of microcrystalline cellulose: take 100g of microcrystalline cellulose and immerse in 1% CaCl 2 Soak in the solution for 30min, then take it out, drain off the surface moisture, put it in a microwave oven, and dry it with microwave puffing until the moisture content is below 5%, to obtain CaCl 2 Adsorbed microcrystalline cellulose. The microcrystalline cellulose treated by this method has good bulkiness, and the adsorption rate to water-soluble mushroom protein is 85%, which is 15% higher than that of common microcrystalline cellulose.
[0017] Take 1kg of shiitake mushroom powder, add 20kg of distilled water, stir and extract in a 24°C water bath for 5.5h, then centrifuge at 3500rpm to collect the supernatant; adjust the pH to 8.5, add 0.03g of alkaline protease per gram of protein, and act in a 50°C water bath for 50min , boiled to kill the enzyme, after cooling, centrifuge to get the supernatant, and the supernatant is successively filtered wit...
Embodiment 2
[0022] CaCl 2 -The preparation of microcrystalline cellulose is the same as in Example 1.
[0023] Take 1kg of shiitake mushroom powder, add 25kg of distilled water, stir and extract in a 30°C water bath for 4 hours, then centrifuge to collect the supernatant, adjust the pH to 9.0, add 0.05g of alkaline protease per gram of protein, modify it in a 55°C water bath for 30 minutes, and boil it off After the enzyme is cooled, the supernatant is obtained by centrifugation, and the supernatant is successively filtered with a 20KDa ultrafiltration membrane and a 500Da nanofiltration membrane to obtain a retentate with an intermediate molecular weight. Concentrate the retentate in vacuum to a protein content of 30%, mix it with 2.5% sodium alginate solution at a volume ratio of 2:1, stir evenly, add 15g of processed CaCl 2 - Embedding with microcrystalline cellulose, then drying and pulverizing to obtain the shiitake mushroom protein embedding with weight loss effect.
[0024] The ...
Embodiment 3
[0026] CaCl 2 -The preparation of microcrystalline cellulose is the same as in Example 1.
[0027] Take 1kg of shiitake mushroom powder, add 15kg of distilled water, extract in a 26°C water bath for 5 hours, centrifuge to collect the supernatant, add 0.06g of alkaline protease per gram of protein, modify at 60°C and pH7.5 for 60min, hydrolyze and boil Inactivate the enzyme for 10 minutes, take the supernatant, filter the supernatant with 20KDa and 500Da ultrafiltration membranes successively to collect the retentate with intermediate molecular weight, and vacuum concentrate the retentate to a protein content of 35%. Mix the concentrated retentate with 2.5% sodium alginate solution at a volume ratio of 2:1, and add 15g of CaCl per 100ml of the mixture of retentate and sodium alginate 2 -Microcrystalline cellulose, mixed evenly, dried and crushed to obtain the mushroom protein-embedded food with weight loss function. The protein modification is fed to animals to show obvious w...
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