Method for Simultaneous Determination of Concentration of Ursolic Acid and Schisandra A, B, C

Abstract

The invention discloses a method for simultaneously measuring the concentrations of monomer components including ursolic acid, schizandrin a, schizandrin b and schizandrin c, belonging to material analysis by using a chromatography. The method disclosed by the invention comprises the following steps: respectively preparing a mixed sample of dogwood and schisandra chinensis and a negative control solution of a sample to be measured, and, after pre-processing, carrying out preparation of a working curve and chromatographic separation and content determination of the four monomer components under the conditions as follows: a mobile phase is the mixture of acetonitrile and 0.1% phosphoric acid aqueous solution with the ratio of 67 to 33; the volume flow rate is 0.8-1.2 mL / min; the ultraviolet detection wavelength is 190-220 nm; and the column temperature is 25-40 DEG C. Simultaneous concentration detection and complete separation of the four monomer components including ursolic acid, schizandrin a, schizandrin b and schizandrin c are realized within a relatively short time under same wavelength chromatographic experimental conditions; the method disclosed by the invention has the advantages of being steady in base line, symmetrical and attractive in peak pattern, simple, convenient, rapid, high in separation degree and sensitivity, good in repeatability, accurate and reliable in data and high in recovery; and therefore, a good quality control detection method is provided.

Description

Technical field [0001] The invention relates to the field of analyzing materials by chromatography, in particular to a method for simultaneously determining the concentration of ursolic acid and schisandrin A, B, and B monomer components. Background technique [0002] Traditional Chinese medicine, especially natural Chinese medicine, has played an important role in the reproduction, survival and development of humans for thousands of years, and Schisandra and Cornus are among the more important medicinal materials. Schisandra is a perennial deciduous vine, belonging to the Magnoliaceae. This medicine has complete functions and all five flavors, so it is called Schisandra. The combination of five flavors in traditional Chinese medicine is unique, and it has a unique effect on yin deficiency and yang deficiency. Schisandra has the effects of restraining the lungs, nourishing the kidneys, producing body fluids, restraining sweat, astringent essence, relieving diarrhea, and calming ...

AI Technical Summary

Problems solved by technology

[0004] The present invention is aimed at overcoming the baseline fluctuations caused by the gradient elution method when simultaneously detecting Schisandra A, B, and C in the prior art, the sensitivity is reduced, and the inability to simultaneously detect ursolic acid and Schisandra A, B, and C four active single substances. Because of the disadvantages of body composition, a method for simultaneously determining the concentration of ursolic acid and Schisandra A, B, and C monomer components by an isocratic elution method is provided

Images