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Method for detecting nucleoside components in Morchella esculenta

A detection method, technology of hickory chick, applied in the detection of nucleoside components in hickory chick, detection of guanosine, uridine and adenosine in the field of three nucleoside components in hickory chick, which can solve the unreachable, nucleoside Low content, asymmetric peak shape and other problems, to achieve the effect of solving stability problems, stable chromatographic baseline, and good stability

Active Publication Date: 2017-03-15
东莞东阳光保健品研发有限公司
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  • Abstract
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AI Technical Summary

Problems solved by technology

There are also many literatures that have studied the nucleoside components in medicinal edible fungi, but the methods for detecting nucleoside components reported in the literature cannot be directly applied to the detection of nucleosides in Morchella. The high performance liquid chromatography method for determination of adenosine and uridine in capsules was directly applied to the detection of three nucleoside components of Morchella fungus, and there were problems such as peak shape asymmetry and baseline drift; The nucleoside components of 0.5% phosphoric acid solution were used to extract the nucleoside components and the content of uridine, inosine, guanosine and adenosine in 4 kinds of Cordyceps medicinal materials were determined, but the extraction method was used to extract morel The nucleoside composition of the results found that the nucleoside content was very low
The 10% methanol extraction method used in many other literatures is also used in the extraction of Morchella nucleoside, and there is also a problem of low content
At the same time, the inventor found in a large number of experimental researches that in the process of methodological investigation of the above-mentioned analysis and detection method, there is a problem of poor stability, and its RSD cannot meet the detection requirement of less than 5%, which may be caused by the nucleosides in the sample. The extraction and processing methods of the class components have a great influence on the determination of their content

Method used

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  • Method for detecting nucleoside components in Morchella esculenta
  • Method for detecting nucleoside components in Morchella esculenta
  • Method for detecting nucleoside components in Morchella esculenta

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1 Morchella nucleoside component extraction method and the optimization of providing solvent

[0037] 1.1 Instruments and reagents:

[0038]Instruments: high-performance liquid chromatograph model is Agilent 1260, electronic balance (1 / 10,000 and 1 / 100,000) model is Mettler Toledo, ultrasonic cleaner model is KQ-500DB, and ultrapure water instrument model is MILLI-Q.

[0039] Reagents: Potassium dihydrogen phosphate was analytical grade, purchased from Chengdu Kelong; acetonitrile was HPLC grade, purchased from Spectrum; uridine, guanosine and adenosine (including analytical grade) were purchased from China Institute for the Control of Pharmaceutical and Biological Products.

[0040] The morel cultured freeze-dried products come from the cultivation base of Ruyuan Nanling Haoshanhaoshui Food Co., Ltd.

[0041] 1.2 Analysis method:

[0042] During the experiment, it was found that different extraction methods and different extraction solvents had an impact o...

Embodiment 2

[0055] Embodiment 2 Discussion on the Stability Variation Trend of Morchella Nucleosides Components Extract

[0056] In the process of methodological verification, it was found that the precision of the obtained test solution was placed for different times by HPLC to detect its precision, and it was found that some data were unqualified, that is, the stability of the extract was not good (less than 4h), so The stability of the extract was investigated, and the change trend of the stability of the extract by water extraction and 20% alcohol extraction were investigated respectively.

[0057] Extract samples according to ① and ③ in the method for extracting nucleoside components from Morchella spp. in Example 1, investigate respectively the changes in the content of nucleoside components contained in the samples of the test product placed at 6°C for 36 hours at intervals of 1 hour At the same time, the changes in the content of the nucleoside components contained in the nucleosi...

Embodiment 3

[0061] Embodiment 3HPLC detects the method establishment of the nucleoside component in hickory chick

[0062] According to Examples 1 and 2, it is determined that the best method for extracting hickory chick samples is: using pure water as a solvent, the ratio of solid to liquid is set to 1:50, ultrasonically extracting for 30 minutes, and placing the water extract at 6°C for 16 to 10 minutes. The stability of nucleoside components in 28h established the HPLC detection method of its Morchella samples.

[0063] Preparation of the test solution: take 0.2 g of Morchella powder passed through a No. 3 sieve, accurately weigh it in a 50 mL Erlenmeyer flask, add 10 mL of ultrapure water, ultrasonically extract at 30°C for 30 min, shake well, take the supernatant through After filtering with a 0.45 μm water filter head, let it stand at 6°C for 16 hours to obtain the solution of the test product to be tested.

[0064] Preparation of nucleoside mixed reference substance solution: Accu...

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Abstract

The invention belongs to the field of medical domestic fungus analysis and detection and relates to a method for simultaneous detection of guanosine, uridine and adenosine in Morchella esculenta by HPLC. The method comprises adding water into Morchella esculenta powder, carrying out constant temperature ultrasonic extraction, carrying out full shaking, taking the supernatant, filtering the supernatant through a water filter head, carrying out standing to obtain a sample solution to be detected, preparing a nucleoside reference solution and detecting nucleoside components in Morchella esculenta through HPLC. The method for detecting nucleoside components in Morchella esculenta has high stability, good repeatability and a good precision, produces a reliable and convenient result and can provide scientific basis for quality control of Morchella esculenta.

Description

technical field [0001] The invention relates to the field of analysis and detection of edible and medicinal fungi, in particular to a method for detecting nucleoside components in morels, in particular to detecting three nucleoside components in morels, guanosine, uridine and adenosine, by HPLC . Background technique [0002] Morel (Morchella esculenta) is a rare edible and medicinal fungus. Traditional Chinese medicine believes that Morchella is mild in nature, sweet and cold in taste, and non-toxic. It has the functions of helping digestion and food, benefiting the stomach, reducing phlegm and regulating qi, nourishing the kidney, strengthening yang, and refreshing the brain. It has high medicinal and edible value and broad development prospects. . Modern medical research shows that Morchella fruiting bodies are rich in protein, nucleosides, fatty acids, carbonates, biotin, crude fiber and other nutrients. However, according to the survey, the current in-depth research ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 周建桥李春红艾中李文佳李文庆钱正明
Owner 东莞东阳光保健品研发有限公司
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