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Portable cholesterol molecular imprinting silk-screen printing electrochemical tester

A technology of screen printing electrodes and molecular imprinting, applied in electrochemical variables of materials, scientific instruments, instruments, etc., can solve the problems of poor cholesterol recognition ability, easy inactivation of enzymes, false negatives, etc., to avoid instability and stability. The effect of short time and convenient detection

Active Publication Date: 2015-04-29
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this detector requires sol-gel with high stability as the enzyme-immobilized matrix, and the enzyme on the electrode is easily inactivated, resulting in false negative results. In addition, because this detection method has poor specificity for cholesterol recognition , in the actual sample detection, there are false positive results

Method used

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  • Portable cholesterol molecular imprinting silk-screen printing electrochemical tester
  • Portable cholesterol molecular imprinting silk-screen printing electrochemical tester
  • Portable cholesterol molecular imprinting silk-screen printing electrochemical tester

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 Preparation of Cholesterol Molecularly Imprinted Screen-Printed Electrode

[0051] Schematic diagram of the preparation of cholesterol molecularly imprinted screen-printed electrode sheet as shown in figure 1 shown. The specific preparation method is as follows:

[0052] (1) Decorate carbon nanotubes on the surface of the working screen-printed electrode: mix 500 mg of multi-walled carbon nanotubes with 60 mL of concentrated nitric acid, boil at 85 degrees for 12 hours, then pass through a 0.22 μm polycarbonate membrane after cooling, wash with water, and blow dry with nitrogen Finally, 5 mg of the prepared carboxylated multi-walled carbon nanotubes were mixed with 10 mL of 1.0 wt % chitosan (acetic acid as a solvent), and then 10 μL was drawn and dropped onto the working electrode, and dried at room temperature.

[0053] (2) Electroplating and depositing gold nanoparticles on the surface of the working screen printing electrode, electroplating method: the w...

Embodiment 2

[0061] Example 2 Application of Cholesterol Molecularly Imprinted Screen Printing Electrode

[0062] The sample test steps are as follows:

[0063] First, a standard (known concentration of cholesterol) molecularly imprinted screen-printed electrode is inserted into the portable detector to calibrate the detection results of the portable detector. Then mix the purified cholesterol sample with an equal volume of conductive solution, the configuration of conductive solution: 2.5mmol L -1 [Fe(CN) 6 ] 3 / 4 and 0.1mol L -1 KCl solution, drop 20 μL of the mixture of cholesterol sample and conductive solution on the surface of the screen-printed electrode of the prepared cholesterol molecularly imprinted membrane, let the static adsorption time: 120s, set the voltage range: 0.1V ~ 0.3V, record the scanning The current peak value is calculated according to the standard curve between the cholesterol concentration and the current peak value built in the cholesterol-specific portable ...

Embodiment 3

[0067] Example 3 Application of Cholesterol Molecularly Imprinted Screen Printing Electrode

[0068] In a portable detector of the present invention, which is a dedicated portable detector for cholesterol, the standard curve between cholesterol concentration and peak current has been built into the detector program.

[0069] The drawing method of this standard curve: prepare different known concentrations (concentration range: 1 * 10 -13 ~1×10 -9 mol L -1 ) cholesterol molecule imprinted screen-printed electrode is inserted into the detector to detect the current peak value formed when electrons flow from the counter electrode, through the conductive liquid, and then from the working electrode back to the workstation. Take the cholesterol concentration as the abscissa, and the peak current value as the ordinate Coordinates, the standard curve is obtained, such as image 3 shown. The standard curve equation that obtains is: I p =-5.033LogC+16.885(R 2 =0.995), the detectio...

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Abstract

The invention discloses a portable cholesterol molecular imprinting silk-screen printing electrochemical tester and belongs to the technical field of rapid detection. A silk-screen printing electrode is polymerized with template molecule cholesterol by electroplating and a mixed solution of a purified sample and conductive liquid is dropwise added on the surface of a working electrode of the silk-screen printing electrode; current of cholesterol can be directly read by the portable cholesterol molecular imprinting silk-screen printing electrochemical tester; a standard curve is corrected according to a silk-screen printing electrode piece with the cholesterol with the standard concentration; and the concentration of the cholesterol in the detected sample is calculated by a built-in program. The tester disclosed by the invention has a simple structure, is portable, sensitive, rapid and accurate, has high specificity and is economical. The tester can be used for directly detecting the cholesterol, has the advantages of portability, sensitivity, rapidness, high specificity and the like, has low price and is suitable for detecting the cholesterol on a base layer or in the field.

Description

technical field [0001] The invention relates to a portable cholesterol molecular imprinting screen printing electrochemical detector, which belongs to the technical field of rapid detection. Background technique [0002] Cholesterol is a white waxy substance in animal tissue, which is a derivative of cyclopentane polyhydrophenanthrene. Cholesterol is an important component of cell membranes and an essential substance for the human body, but excess cholesterol can cause heart and brain hemorrhage. In recent years, there are several common types of diseases caused by high cholesterol: high blood pressure, kidney disease, prostate cancer, destruction of human bones, periodontal disease, tinnitus or ear fullness, high cholesterol can also cause ischemic heart disease (angina pectoris, myocardial infarction), cerebral infarction, intermittent claudication and gallstones. [0003] There are various detection methods for cholesterol with different principles. Each method has its ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/327
Inventor 孙秀兰纪剑张银志孙嘉笛孙超
Owner JIANGNAN UNIV
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