Streptomyces lavendulae and antibiological inoculant as well as application of antibiological inoculant

A technology of Streptomyces lavender gray and biocontrol fungicides, applied in the fields of application, biocide, fungicide, etc., can solve the problems of strawberry fruit pesticide residues, affecting food environmental safety and economic benefits, and pathogenic bacteria producing drug resistance, etc. Achieve the effect of no fruit pesticide residue

Inactive Publication Date: 2015-04-29
EASTERN LIAONING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disease is especially susceptible to the strawberry variety "Hongyan", causing a large number of dead seedlings and seriously affecting strawberry production
[0003] At present, the disease is mostly controlled by field management and chemical agents, but the effect is not satisfactory
Long-term use of chemical pesticides leads to drug resistance of pathogenic bacteria, resulting in pesticide residues in strawberry fruits, seriously affecting food, environmental safety and economic benefits

Method used

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  • Streptomyces lavendulae and antibiological inoculant as well as application of antibiological inoculant
  • Streptomyces lavendulae and antibiological inoculant as well as application of antibiological inoculant
  • Streptomyces lavendulae and antibiological inoculant as well as application of antibiological inoculant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 The separation and screening of strawberry anthracnose antibody antagonism strains

[0022] In June 2013, the five -point sample method was adopted from the strawberry cultivation base of Donggang City, Liaoning Province to collect soil samples, and it was dry naturally.Take 10g of soil samples, place it in a triangular bottle equipped with 90ml of sterile water, and shake on the bed for 20min.After setting 30s, dilute 10 times in order and prepare 10 -4 Diluent.Trace pipette to absorb 10 -4 The diluted solution is 0.1ml to Gao's No. 1 medium solid flat plate, and the sterile coating is evenly coated.25 ° C in the constant temperature training box inverted for 3-5 days.The strains with different colonies on the tablet are transferred to the Gao's No. 1 medium to obtain pure culture, sterilized paraffin oil seal, and retained at 4 ° C.Gao's No. 1 medium: 20.0g of soluble starch, 1.0g potassium nitrate, 0.5g sodium chloride, K 2 HPO 4 · 3H 2 O 0.5g, MGSO 4 · 7H 2 O ...

Embodiment 2

[0025] Example 2 Appraisal of an antagonistic strain

[0026] 1. Moderate identification and biochemical characteristics

[0027] Porching form: The strain DTJ-24 is based on Gao's No. 1 medium, the amelioline color of the auriculum, the base in the base, and the soluble pigment. See figure 1 It

[0028] Micro form: There is no diaphragm in the base in the microscope and is constantly cracking; the vitamin mycelium has many branches, and the branches are short, the spore wire is long, the spiral, the spore is oval to the column shape, the surface is smooth, see figure 2 It

[0029] Biochemical characteristics: The strain DTJ-24 can make gelatin liquefaction and starch disintegrate.Sulfid sulfide, can decompose cellulose, milk stems, and non -solidification.You can use glucose and fructose, which is not good for sucrose ,osol, mannitol, cotton sugar, D-hypoga.

[0030] 2. 16srdna sequence analysis

[0031] The strain DTJ-24 was inoculated in the Gao's liquid medium, 3D was cultivat...

Embodiment 3

[0034] Example 3 Preparation of Bacteriogenic Bacterium

[0035] DTJ-24, which was stored in the low-purple-gray mildew strain stored in the low temperature of the inclined plane to the DTJ-24 of the Gao's No. 1 tablet, trains 48 h for activation.25 ° C, 120r / min, oscillation of bed oscillating 3D for seed expansion and cultivation to obtain seed liquid.

[0036] The seed liquid is inoculated in the fermentation medium at a vaccination amount of 4%(V / V). The fermentation tank medium is 70%installed, the fermentation conditions are 25 ° C, the amount of ventilation is 18%, the stirring speed is 120r / min, the fermentation cycle, the fermentation cycleIt is 3D, the number of bacteria in the fermentation solution is 6 × 10 9 CFU / ml, the fermentation solution and the calcium powder of the matrix microfladeed calcium carbonate are evenly mixed and dried, and the biochemical agent is obtained. The number of live bacteria in the bacteria is 1 × 10 9 CFU / ml.

[0037] Seed medium formula is...

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Abstract

The invention discloses a streptomyces lavendulae DTJ-24 of which the collection number is CGMCC No.9972, an antibiological inoculants containing the streptomyces lavendulae, and application of the antibiological inoculant to control of a strawberry anthracnose. After being activated, the streptomyces lavendulae DTJ-24 is inoculated in a seed medium at 20% of inoculation quantity, and is cultivated for 3 days at the speed of 120 r / min at 25 DEG C to obtain a seed solution; the seed solution is inoculated in a fermentation medium at 4% of inoculation quantity, the quantity of the mediums in a fermentation tank is 70%, the fermentation condition is that the temperature is 25 DEG C, the throughput is 18%, the stirring speed is 120 r / min, the fermentation time is 3 days, and the fermentation broth and a base material are equivalently and uniformly mixed and dried to prepare the antibiological inoculant. The foliage spray or transplantation is carried out on the antibiological inoculant, through the root-irrigation treatment, the strawberry anthracnose can be effectively controlled, and the control effect reaches 78.3%. The antibiological inoculant is simple in preparation process, low in cost, and convenient to use, can reduce the use of chemical pesticides and pesticide residues on fruit during the strawberry cultivation process, and has the advantages of safety and environmental protection.

Description

Technical field [0001] The present invention involves a DTJ-24, which has antagonistic effects on strawberry anthracnose, and biochemozen can prevent strawberry anthracnose, which belongs to the field of biotechnology. Background technique [0002] Strawberry anthracnose is an important disease in the strawberry area in my country.The disease is the most harmful in the strawberry seedling stage and transplantation period. It often invades stolons, petioles, leaves, and roots, producing local anthracnose spots, resulting in rotting roots and death.When the humidity is high, the orange -red sticky spore pile can be seen.The pathogenic bacteria are mainly three types of strawberry and ample fungus, gluejohumin and spine anthrax.The disease is particularly susceptible to the "red face" of strawberries, causing a large number of dead seedlings, which seriously affects strawberry production. [0003] At present, the disease is mostly used in field management and chemicals, but the effe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/465
CPCA01N63/00C12N1/20C12N1/205C12R2001/465
Inventor 李娟王旭刘丹梅孙书伟季长波刘晓红韦月平李莉姜路路
Owner EASTERN LIAONING UNIV
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